Decellularization of the Murine Cardiopulmonary Complex
(2021) In Journal of visualized experiments : JoVE 171.- Abstract
- We present here a decellularization protocol for mouse heart and lungs. It produces structural ECM scaffolds that can be used to analyze ECM topology and composition. It is based on a microsurgical procedure designed to catheterize the trachea and aorta of a euthanized mouse to perfuse the heart and lungs with decellularizing agents. The decellularized cardiopulmonary complex can subsequently be immunostained to reveal the location of structural ECM proteins. The whole procedure can be completed in 4 days.
The ECM scaffolds resulting from this protocol are free of dimensional distortions. The absence of cells enables structural examination of ECM structures down to submicron resolution in 3D. This protocol can be applied to... (More) - We present here a decellularization protocol for mouse heart and lungs. It produces structural ECM scaffolds that can be used to analyze ECM topology and composition. It is based on a microsurgical procedure designed to catheterize the trachea and aorta of a euthanized mouse to perfuse the heart and lungs with decellularizing agents. The decellularized cardiopulmonary complex can subsequently be immunostained to reveal the location of structural ECM proteins. The whole procedure can be completed in 4 days.
The ECM scaffolds resulting from this protocol are free of dimensional distortions. The absence of cells enables structural examination of ECM structures down to submicron resolution in 3D. This protocol can be applied to healthy and diseased tissue from mice as young as 4-weeks old, including mouse models of fibrosis and cancer, opening the way to determine ECM remodeling associated with cardiopulmonary disease. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/68b460c4-2ce4-4adf-8117-d51c2674cf2d
- author
- Mayorca-guiliani, Alejandro E ; Rafaeva, Maria ; Willacy, Oliver ; Madsen, Chris LU ; Reuten, Raphael and Erler, Janine T.
- organization
- publishing date
- 2021-05-30
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of visualized experiments : JoVE
- volume
- 171
- publisher
- JoVE
- external identifiers
-
- scopus:85108189346
- pmid:34125099
- ISSN
- 1940-087X
- DOI
- 10.3791/61854
- language
- English
- LU publication?
- yes
- id
- 68b460c4-2ce4-4adf-8117-d51c2674cf2d
- date added to LUP
- 2021-05-31 08:07:34
- date last changed
- 2022-08-02 08:08:16
@article{68b460c4-2ce4-4adf-8117-d51c2674cf2d, abstract = {{We present here a decellularization protocol for mouse heart and lungs. It produces structural ECM scaffolds that can be used to analyze ECM topology and composition. It is based on a microsurgical procedure designed to catheterize the trachea and aorta of a euthanized mouse to perfuse the heart and lungs with decellularizing agents. The decellularized cardiopulmonary complex can subsequently be immunostained to reveal the location of structural ECM proteins. The whole procedure can be completed in 4 days.<br/><br/>The ECM scaffolds resulting from this protocol are free of dimensional distortions. The absence of cells enables structural examination of ECM structures down to submicron resolution in 3D. This protocol can be applied to healthy and diseased tissue from mice as young as 4-weeks old, including mouse models of fibrosis and cancer, opening the way to determine ECM remodeling associated with cardiopulmonary disease.}}, author = {{Mayorca-guiliani, Alejandro E and Rafaeva, Maria and Willacy, Oliver and Madsen, Chris and Reuten, Raphael and Erler, Janine T.}}, issn = {{1940-087X}}, language = {{eng}}, month = {{05}}, publisher = {{JoVE}}, series = {{Journal of visualized experiments : JoVE}}, title = {{Decellularization of the Murine Cardiopulmonary Complex}}, url = {{http://dx.doi.org/10.3791/61854}}, doi = {{10.3791/61854}}, volume = {{171}}, year = {{2021}}, }