The secreted serine protease xHtrA1 stimulates long-range FGF signaling in the early Xenopus embryo
Hou, Shirui LU ; Maccarana, Marco LU ; Tan Grahn, Hooi Min LU
; Strate, Ina
LU
and Pera, Edgar
LU
(2007)
In Developmental Cell
13(2).
p.226-241
- Abstract
- We found that the secreted serine protease xHtrA1, expressed in the early embryo and transcriptionally activated by FGF signals, promotes posterior development in mRNA-injected Xenopus embryos. xHtrA1 mRNA led to the induction of secondary tail-like structures, expansion of mesoderm, and formation of ectopic neurons in an FGF-dependent manner. An antisense morpholino oligonucleotide or a neutralizing antibody against xHtrA1 had the opposite effects. xHtrA1 activates FGF/ ERK signaling and the transcription of FGF genes. We show that Xenopus Biglycan, Syndecan-4, and Glypican-4 are proteolytic targets of xHtrA1 and that heparan sulfate and dermatan sulfate trigger posteriorization, mesoderm induction, and neuronal differentiation via the... (More)
- We found that the secreted serine protease xHtrA1, expressed in the early embryo and transcriptionally activated by FGF signals, promotes posterior development in mRNA-injected Xenopus embryos. xHtrA1 mRNA led to the induction of secondary tail-like structures, expansion of mesoderm, and formation of ectopic neurons in an FGF-dependent manner. An antisense morpholino oligonucleotide or a neutralizing antibody against xHtrA1 had the opposite effects. xHtrA1 activates FGF/ ERK signaling and the transcription of FGF genes. We show that Xenopus Biglycan, Syndecan-4, and Glypican-4 are proteolytic targets of xHtrA1 and that heparan sulfate and dermatan sulfate trigger posteriorization, mesoderm induction, and neuronal differentiation via the FGIF signaling pathway. The results are consistent with a mechanism by which xHtrA1, through cleaving proteoglycans, releases cellsurface-bound FGF ligands and stimulates long-range FGF signaling. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/692758
- author
- Hou, Shirui
LU
; Maccarana, Marco
LU
; Tan Grahn, Hooi Min
LU
; Strate, Ina
LU
and Pera, Edgar
LU
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Developmental Cell
- volume
- 13
- issue
- 2
- pages
- 226 - 241
- publisher
- Cell Press
- external identifiers
-
- wos:000248664300010
- scopus:34547475712
- ISSN
- 1534-5807
- DOI
- 10.1016/j.devcel.2007.07.001
- language
- English
- LU publication?
- yes
- id
- 7bcce079-e86e-4cf5-8034-8204fcee06a4 (old id 692758)
- date added to LUP
- 2016-04-01 11:44:15
- date last changed
- 2022-08-20 21:10:57
@article{7bcce079-e86e-4cf5-8034-8204fcee06a4,
abstract = {{We found that the secreted serine protease xHtrA1, expressed in the early embryo and transcriptionally activated by FGF signals, promotes posterior development in mRNA-injected Xenopus embryos. xHtrA1 mRNA led to the induction of secondary tail-like structures, expansion of mesoderm, and formation of ectopic neurons in an FGF-dependent manner. An antisense morpholino oligonucleotide or a neutralizing antibody against xHtrA1 had the opposite effects. xHtrA1 activates FGF/ ERK signaling and the transcription of FGF genes. We show that Xenopus Biglycan, Syndecan-4, and Glypican-4 are proteolytic targets of xHtrA1 and that heparan sulfate and dermatan sulfate trigger posteriorization, mesoderm induction, and neuronal differentiation via the FGIF signaling pathway. The results are consistent with a mechanism by which xHtrA1, through cleaving proteoglycans, releases cellsurface-bound FGF ligands and stimulates long-range FGF signaling.}},
author = {{Hou, Shirui and Maccarana, Marco and Tan Grahn, Hooi Min and Strate, Ina and Pera, Edgar}},
issn = {{1534-5807}},
language = {{eng}},
number = {{2}},
pages = {{226--241}},
publisher = {{Cell Press}},
series = {{Developmental Cell}},
title = {{The secreted serine protease xHtrA1 stimulates long-range FGF signaling in the early Xenopus embryo}},
url = {{http://dx.doi.org/10.1016/j.devcel.2007.07.001}},
doi = {{10.1016/j.devcel.2007.07.001}},
volume = {{13}},
year = {{2007}},
}