Generation of an induced pluripotent stem cell line (CSC-32) from a patient with Parkinson's disease carrying a heterozygous variation p.A53T in the SNCA gene
(2020) In Stem Cell Research 43.- Abstract
Here, we describe the generation of an induced pluripotent stem cell (iPSC) line, from a male patient diagnosed with Parkinson's disease (PD). The patient carries a heterozygous variation p.A53T in the SNCA gene. Skin fibroblasts were reprogrammed using the non-integrating Sendai virus technology to deliver OCT3/4, SOX2, c-MYC and KLF4 factors. The generated iPSC line (CSC-32) preserved the mutation, displayed expression of common pluripotency markers, differentiated into derivatives of the three germ layers, and exhibited a normal karyotype. The clone CSC-32B is presented thereafter; it can be used to study the mechanisms underlying PD pathogenesis.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/70ca534e-e8db-4b5f-a5db-4c49676738ed
- author
- Azevedo, Carla LU ; Chumarina, Margarita LU ; Serafimova, Evgenija LU ; Goldwurm, Stefano ; Collin, Anna LU ; Roybon, Laurent LU ; Savchenko, Ekaterina LU and Pomeshchik, Yuriy LU
- organization
- publishing date
- 2020-03
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Stem Cell Research
- volume
- 43
- article number
- 101694
- publisher
- Elsevier
- external identifiers
-
- scopus:85077915033
- pmid:31954327
- ISSN
- 1873-5061
- DOI
- 10.1016/j.scr.2019.101694
- language
- English
- LU publication?
- yes
- id
- 70ca534e-e8db-4b5f-a5db-4c49676738ed
- date added to LUP
- 2020-01-30 17:37:43
- date last changed
- 2024-09-18 18:02:41
@article{70ca534e-e8db-4b5f-a5db-4c49676738ed, abstract = {{<p>Here, we describe the generation of an induced pluripotent stem cell (iPSC) line, from a male patient diagnosed with Parkinson's disease (PD). The patient carries a heterozygous variation p.A53T in the SNCA gene. Skin fibroblasts were reprogrammed using the non-integrating Sendai virus technology to deliver OCT3/4, SOX2, c-MYC and KLF4 factors. The generated iPSC line (CSC-32) preserved the mutation, displayed expression of common pluripotency markers, differentiated into derivatives of the three germ layers, and exhibited a normal karyotype. The clone CSC-32B is presented thereafter; it can be used to study the mechanisms underlying PD pathogenesis.</p>}}, author = {{Azevedo, Carla and Chumarina, Margarita and Serafimova, Evgenija and Goldwurm, Stefano and Collin, Anna and Roybon, Laurent and Savchenko, Ekaterina and Pomeshchik, Yuriy}}, issn = {{1873-5061}}, language = {{eng}}, publisher = {{Elsevier}}, series = {{Stem Cell Research}}, title = {{Generation of an induced pluripotent stem cell line (CSC-32) from a patient with Parkinson's disease carrying a heterozygous variation p.A53T in the SNCA gene}}, url = {{http://dx.doi.org/10.1016/j.scr.2019.101694}}, doi = {{10.1016/j.scr.2019.101694}}, volume = {{43}}, year = {{2020}}, }