The lipolytic degradation of highly structured cubic micellar nanoparticles of soy phosphatidylcholine and glycerol dioleate by phospholipase A2 and triacylglycerol lipase
(2018) In Chemistry and Physics of Lipids 211. p.86-92- Abstract
The effects of different lipolytic enzymes on the structure of lipid liquid crystalline nano-particles (LCNP) have been investigated by cryogenic transmission electron microscopy (cryo-TEM) and synchrotron small angle X-ray diffraction (SAXD). Here we used highly structured cubic micellar (Fd3m) nanoparticles of 50/50 (wt%/wt%) soy phosphatidyl choline (SPC)/glycerol dioleate (GDO) as substrate. Two types of lipolytic enzymes were used, phospholipase A2 (PLA2) that catalyses degradation of the phospholipid component, SPC, and porcine pancreatic triacylglycerol lipase (TGL) that facilitate the hydrolysis of the diglyceride, GDO. Evolution of the structure was found to be very different and linked to specificity of... (More)
The effects of different lipolytic enzymes on the structure of lipid liquid crystalline nano-particles (LCNP) have been investigated by cryogenic transmission electron microscopy (cryo-TEM) and synchrotron small angle X-ray diffraction (SAXD). Here we used highly structured cubic micellar (Fd3m) nanoparticles of 50/50 (wt%/wt%) soy phosphatidyl choline (SPC)/glycerol dioleate (GDO) as substrate. Two types of lipolytic enzymes were used, phospholipase A2 (PLA2) that catalyses degradation of the phospholipid component, SPC, and porcine pancreatic triacylglycerol lipase (TGL) that facilitate the hydrolysis of the diglyceride, GDO. Evolution of the structure was found to be very different and linked to specificity of the two types of enzymes. PLA2, which hydrolyses the lamellar forming component, SPC, induces a reversed micellar lipid phase, while TGL which hydrolysis the reverse phase forming compound, GDO, induces a lamellar phase.
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- author
- Wadsäter, Maria LU ; Barauskas, Justas LU ; Tiberg, Fredrik LU and Nylander, Tommy LU
- organization
- publishing date
- 2018
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Cryogenic transmission electron microscopy, Lipid liquid crystalline nano-particles, Lipolytic enzymes, Phospholipase A, Small angle X-ray diffraction, Triacylglycerol lipase
- in
- Chemistry and Physics of Lipids
- volume
- 211
- pages
- 86 - 92
- publisher
- Elsevier
- external identifiers
-
- pmid:29132829
- scopus:85034242000
- ISSN
- 0009-3084
- DOI
- 10.1016/j.chemphyslip.2017.11.011
- language
- English
- LU publication?
- yes
- id
- 742df5e9-4f51-4a02-a6d8-5a18cb349d1a
- date added to LUP
- 2017-12-11 07:50:05
- date last changed
- 2024-10-14 19:05:51
@article{742df5e9-4f51-4a02-a6d8-5a18cb349d1a, abstract = {{<p>The effects of different lipolytic enzymes on the structure of lipid liquid crystalline nano-particles (LCNP) have been investigated by cryogenic transmission electron microscopy (cryo-TEM) and synchrotron small angle X-ray diffraction (SAXD). Here we used highly structured cubic micellar (Fd3m) nanoparticles of 50/50 (wt%/wt%) soy phosphatidyl choline (SPC)/glycerol dioleate (GDO) as substrate. Two types of lipolytic enzymes were used, phospholipase A<sub>2</sub> (PLA<sub>2</sub>) that catalyses degradation of the phospholipid component, SPC, and porcine pancreatic triacylglycerol lipase (TGL) that facilitate the hydrolysis of the diglyceride, GDO. Evolution of the structure was found to be very different and linked to specificity of the two types of enzymes. PLA<sub>2</sub>, which hydrolyses the lamellar forming component, SPC, induces a reversed micellar lipid phase, while TGL which hydrolysis the reverse phase forming compound, GDO, induces a lamellar phase.</p>}}, author = {{Wadsäter, Maria and Barauskas, Justas and Tiberg, Fredrik and Nylander, Tommy}}, issn = {{0009-3084}}, keywords = {{Cryogenic transmission electron microscopy; Lipid liquid crystalline nano-particles; Lipolytic enzymes; Phospholipase A; Small angle X-ray diffraction; Triacylglycerol lipase}}, language = {{eng}}, pages = {{86--92}}, publisher = {{Elsevier}}, series = {{Chemistry and Physics of Lipids}}, title = {{The lipolytic degradation of highly structured cubic micellar nanoparticles of soy phosphatidylcholine and glycerol dioleate by phospholipase A<sub>2</sub> and triacylglycerol lipase}}, url = {{http://dx.doi.org/10.1016/j.chemphyslip.2017.11.011}}, doi = {{10.1016/j.chemphyslip.2017.11.011}}, volume = {{211}}, year = {{2018}}, }