A novel variant of Thermotoga neapolitana beta-glucosidase B is an efficient catalyst for the synthesis of alkyl glucosides by transglycosylation
Turner, Pernilla LU ; Svensson, David LU ; Adlercreutz, Patrick LU
and Nordberg Karlsson, Eva
LU
(2007)
In Journal of Biotechnology
130(1).
p.67-74
- Abstract
- Alkyl glycosides are surfactants with good biodegradability and low toxicity, attractive to produce by an enzymatic method to get a well-defined product. In this paper, we report a novel thermostable variant of a family 3 β-glucosidase to be an efficient catalyst in alkyl-glucoside forming reactions using transglycosylation with hexanol or octanol as the acceptor molecule. The enzyme has an apparent optimum for hydrolysis at 90 °C, which coincides with its unfolding temperature. The total activity is lower at lower temperature (60 °C), but the ratio of alcoholysis/hydrolysis is slightly more favourable. This ratio is however more heavily influenced by the water content and the pH. Optimal reaction conditions for hexyl glucoside synthesis... (More)
- Alkyl glycosides are surfactants with good biodegradability and low toxicity, attractive to produce by an enzymatic method to get a well-defined product. In this paper, we report a novel thermostable variant of a family 3 β-glucosidase to be an efficient catalyst in alkyl-glucoside forming reactions using transglycosylation with hexanol or octanol as the acceptor molecule. The enzyme has an apparent optimum for hydrolysis at 90 °C, which coincides with its unfolding temperature. The total activity is lower at lower temperature (60 °C), but the ratio of alcoholysis/hydrolysis is slightly more favourable. This ratio is however more heavily influenced by the water content and the pH. Optimal reaction conditions for hexyl glucoside synthesis from p-nitrophenyl-β-glucopyranoside were a water/hexanol two-phase system containing 16% (v/v) water, pH 5.8, and a temperature of 60 °C. Under these conditions, the total initial reaction rate was 153 μmol min−1 mg−1 and the alcoholysis/hydrolysis ratio was 5.1. Comparing with alcoholysis/hydrolysis ratios of other β-glycosidases, TnBgl3B can be considered to be a very promising catalyst for alkyl glucoside production. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/745487
- author
- Turner, Pernilla
LU
; Svensson, David
LU
; Adlercreutz, Patrick
LU
and Nordberg Karlsson, Eva
LU
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- β-Glucosidase, Alkyl glycosides, Glycoside hydrolase family 3, Thermostable enzyme, Biosynthesis
- in
- Journal of Biotechnology
- volume
- 130
- issue
- 1
- pages
- 67 - 74
- publisher
- Elsevier
- external identifiers
-
- scopus:34247375264
- wos:000246937700008
- pmid:17448557
- ISSN
- 1873-4863
- DOI
- 10.1016/j.jbiotec.2007.02.016
- language
- English
- LU publication?
- yes
- id
- dccf3070-9d91-4dfa-8a5b-8caba7b7c9dd (old id 745487)
- date added to LUP
- 2016-04-01 11:45:55
- date last changed
- 2022-04-28 19:39:06
@article{dccf3070-9d91-4dfa-8a5b-8caba7b7c9dd,
abstract = {{Alkyl glycosides are surfactants with good biodegradability and low toxicity, attractive to produce by an enzymatic method to get a well-defined product. In this paper, we report a novel thermostable variant of a family 3 β-glucosidase to be an efficient catalyst in alkyl-glucoside forming reactions using transglycosylation with hexanol or octanol as the acceptor molecule. The enzyme has an apparent optimum for hydrolysis at 90 °C, which coincides with its unfolding temperature. The total activity is lower at lower temperature (60 °C), but the ratio of alcoholysis/hydrolysis is slightly more favourable. This ratio is however more heavily influenced by the water content and the pH. Optimal reaction conditions for hexyl glucoside synthesis from p-nitrophenyl-β-glucopyranoside were a water/hexanol two-phase system containing 16% (v/v) water, pH 5.8, and a temperature of 60 °C. Under these conditions, the total initial reaction rate was 153 μmol min−1 mg−1 and the alcoholysis/hydrolysis ratio was 5.1. Comparing with alcoholysis/hydrolysis ratios of other β-glycosidases, TnBgl3B can be considered to be a very promising catalyst for alkyl glucoside production.}},
author = {{Turner, Pernilla and Svensson, David and Adlercreutz, Patrick and Nordberg Karlsson, Eva}},
issn = {{1873-4863}},
keywords = {{β-Glucosidase; Alkyl glycosides; Glycoside hydrolase family 3; Thermostable enzyme; Biosynthesis}},
language = {{eng}},
number = {{1}},
pages = {{67--74}},
publisher = {{Elsevier}},
series = {{Journal of Biotechnology}},
title = {{A novel variant of Thermotoga neapolitana beta-glucosidase B is an efficient catalyst for the synthesis of alkyl glucosides by transglycosylation}},
url = {{http://dx.doi.org/10.1016/j.jbiotec.2007.02.016}},
doi = {{10.1016/j.jbiotec.2007.02.016}},
volume = {{130}},
year = {{2007}},
}