Cloning and sequence of a thermostable multidomain xylanase from the bacterium Rhodothermus marinus
(1997) In Biochimica et Biophysica Acta - Gene Structure and Expression 1353(2). p.118-124- Abstract
The gene (xyn1) encoding a Rhodothermus marinus xylanase has been cloned and expressed in Escherichia coli. The gene comprises 5 different domains in an unusual combination. The cellulose binding domains (CBDs) encoded by xyn1 are repeated in tandem at the N-terminus and show similarity with the CBD family IV. The xyn1-gene is the first example encoding a CBD family IV in combination with a xylan hydrolyzing catalytic domain of the glycosyl hydrolase family 10.
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https://lup.lub.lu.se/record/8336abeb-7dfc-4348-b3f1-7314c8f2eaa9
- author
- Karlsson, Eva Nordberg LU ; Bartonek-Roxå, Eva LU and Holst, Olle LU
- organization
- publishing date
- 1997-08-07
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- (Rhodothermus marinus), Cellulose binding domain family IV, Glycosyl hydrolase family 10, Xylanase
- in
- Biochimica et Biophysica Acta - Gene Structure and Expression
- volume
- 1353
- issue
- 2
- pages
- 7 pages
- publisher
- Elsevier
- external identifiers
-
- pmid:9294005
- scopus:0030800010
- ISSN
- 0167-4781
- DOI
- 10.1016/S0167-4781(97)00093-6
- language
- English
- LU publication?
- yes
- id
- 8336abeb-7dfc-4348-b3f1-7314c8f2eaa9
- date added to LUP
- 2018-11-14 21:14:29
- date last changed
- 2024-01-15 05:01:33
@article{8336abeb-7dfc-4348-b3f1-7314c8f2eaa9, abstract = {{<p>The gene (xyn1) encoding a Rhodothermus marinus xylanase has been cloned and expressed in Escherichia coli. The gene comprises 5 different domains in an unusual combination. The cellulose binding domains (CBDs) encoded by xyn1 are repeated in tandem at the N-terminus and show similarity with the CBD family IV. The xyn1-gene is the first example encoding a CBD family IV in combination with a xylan hydrolyzing catalytic domain of the glycosyl hydrolase family 10.</p>}}, author = {{Karlsson, Eva Nordberg and Bartonek-Roxå, Eva and Holst, Olle}}, issn = {{0167-4781}}, keywords = {{(Rhodothermus marinus); Cellulose binding domain family IV; Glycosyl hydrolase family 10; Xylanase}}, language = {{eng}}, month = {{08}}, number = {{2}}, pages = {{118--124}}, publisher = {{Elsevier}}, series = {{Biochimica et Biophysica Acta - Gene Structure and Expression}}, title = {{Cloning and sequence of a thermostable multidomain xylanase from the bacterium Rhodothermus marinus}}, url = {{http://dx.doi.org/10.1016/S0167-4781(97)00093-6}}, doi = {{10.1016/S0167-4781(97)00093-6}}, volume = {{1353}}, year = {{1997}}, }