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A high-throughput deterministic lateral displacement device for rapid and sensitive field-diagnosis of sleeping sickness

Holm, Stefan H. LU ; Beech, Jason P. LU ; Barrett, Michael P. and Tegenfeldt, Jonas O. LU orcid (2012) 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 p.530-532
Abstract

We present a simple and rapid microfluidic device capable of extracting and concentrating the parasite causing the fatal disease sleeping sickness (SS) from blood. The device is based on deterministic lateral displacement (DLD) and constructed with a single inlet with flow induced by an ordinary syringe. The simplicity is crucial as the device is intended for use in the resource depraved areas where the disease is endemic. With only one inlet an intricate design with multiple depths has been utilized to create a cell free stream from the blood plasma into which the parasites are forced and subsequently collected in a detection region. In order to maximize the sample volume up to 10 device layers were stacked on top of each other which... (More)

We present a simple and rapid microfluidic device capable of extracting and concentrating the parasite causing the fatal disease sleeping sickness (SS) from blood. The device is based on deterministic lateral displacement (DLD) and constructed with a single inlet with flow induced by an ordinary syringe. The simplicity is crucial as the device is intended for use in the resource depraved areas where the disease is endemic. With only one inlet an intricate design with multiple depths has been utilized to create a cell free stream from the blood plasma into which the parasites are forced and subsequently collected in a detection region. In order to maximize the sample volume up to 10 device layers were stacked on top of each other which resulted in a throughput of ∼10 μL/min. This allowed for an approximate time per test of below 15 min.

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Please use this url to cite or link to this publication:
author
; ; and
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
Deterministic lateral displacement, High-throughput, Multilayered, Point-of-care, Separation science, Shape sorting
host publication
Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
pages
3 pages
publisher
Chemical and Biological Microsystems Society
conference name
16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
conference location
Okinawa, Japan
conference dates
2012-10-28 - 2012-11-01
external identifiers
  • scopus:84901809445
ISBN
9780979806452
language
English
LU publication?
yes
id
89bc4122-a505-4263-b8d9-477ff1c03cd8
date added to LUP
2018-10-20 08:52:31
date last changed
2023-09-22 10:01:48
@inproceedings{89bc4122-a505-4263-b8d9-477ff1c03cd8,
  abstract     = {{<p>We present a simple and rapid microfluidic device capable of extracting and concentrating the parasite causing the fatal disease sleeping sickness (SS) from blood. The device is based on deterministic lateral displacement (DLD) and constructed with a single inlet with flow induced by an ordinary syringe. The simplicity is crucial as the device is intended for use in the resource depraved areas where the disease is endemic. With only one inlet an intricate design with multiple depths has been utilized to create a cell free stream from the blood plasma into which the parasites are forced and subsequently collected in a detection region. In order to maximize the sample volume up to 10 device layers were stacked on top of each other which resulted in a throughput of ∼10 μL/min. This allowed for an approximate time per test of below 15 min.</p>}},
  author       = {{Holm, Stefan H. and Beech, Jason P. and Barrett, Michael P. and Tegenfeldt, Jonas O.}},
  booktitle    = {{Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012}},
  isbn         = {{9780979806452}},
  keywords     = {{Deterministic lateral displacement; High-throughput; Multilayered; Point-of-care; Separation science; Shape sorting}},
  language     = {{eng}},
  month        = {{01}},
  pages        = {{530--532}},
  publisher    = {{Chemical and Biological Microsystems Society}},
  title        = {{A high-throughput deterministic lateral displacement device for rapid and sensitive field-diagnosis of sleeping sickness}},
  year         = {{2012}},
}