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Dynamics of a Staphylococcus aureus infective endocarditis simulation model

Schwartz, Franziska A. ; Nielsen, Luna ; Andersen, Jessica Struve ; Bock, Magnus ; Christophersen, Lars ; Sunnerhagen, Torgny LU orcid ; Lerche, Christian Johann ; Bay, Lene ; Bundgaard, Henning and Høiby, Niels , et al. (2022) In APMIS 130(8). p.515-523
Abstract
Infective endocarditis (IE) is a serious infection of the inner surface of heart, resulting from minor lesions in the endocardium. The damage induces a healing reaction, which leads to recruitment of fibrin and immune cells. This sterile healing vegetation can be colonized during temporary bacteremia, inducing IE. We have previously established a novel in vitro IE model using a simulated IE vegetation (IEV) model produced from whole venous blood, on which we achieved stable bacterial colonization after 24h. The bacteria were organized in biofilm aggregates and displayed increased tolerance towards antibiotics. In this current study, we aimed at further characterizing the time course of biofilm formation and the impact on antibiotic... (More)
Infective endocarditis (IE) is a serious infection of the inner surface of heart, resulting from minor lesions in the endocardium. The damage induces a healing reaction, which leads to recruitment of fibrin and immune cells. This sterile healing vegetation can be colonized during temporary bacteremia, inducing IE. We have previously established a novel in vitro IE model using a simulated IE vegetation (IEV) model produced from whole venous blood, on which we achieved stable bacterial colonization after 24h. The bacteria were organized in biofilm aggregates and displayed increased tolerance towards antibiotics. In this current study, we aimed at further characterizing the time course of biofilm formation and the impact on antibiotic tolerance development. We found that a S. aureus reference strain, as well as three clinical IE isolates formed biofilms on the IEV after 6h. When treatment was initiated immediately after infection, the antibiotic effect was significantly higher than when treatment was started after the biofilm was allowed to mature. We could follow the biofilm development microscopically by visualizing growing bacterial aggregates on the IEV. The findings indicate that mature, antibiotic-tolerant biofilms can be formed in our model already after 6h, accelerating the screening for optimal treatment strategies for IE. (Less)
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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
APMIS
volume
130
issue
8
pages
515 - 523
publisher
John Wiley & Sons Inc.
external identifiers
  • scopus:85130719962
  • pmid:35460117
ISSN
0903-4641
DOI
10.1111/apm.13231
project
Matters of the heart- Biofilm on cardiovascular prostheses, their microbiological properties, and antibiotic tolerance
language
English
LU publication?
yes
id
8ef21a74-1d83-4d8b-a5c9-8b172bac8eb3
date added to LUP
2022-04-23 15:07:25
date last changed
2022-10-31 14:58:11
@article{8ef21a74-1d83-4d8b-a5c9-8b172bac8eb3,
  abstract     = {{Infective endocarditis (IE) is a serious infection of the inner surface of heart, resulting from minor lesions in the endocardium. The damage induces a healing reaction, which leads to recruitment of fibrin and immune cells. This sterile healing vegetation can be colonized during temporary bacteremia, inducing IE. We have previously established a novel in vitro IE model using a simulated IE vegetation (IEV) model produced from whole venous blood, on which we achieved stable bacterial colonization after 24h. The bacteria were organized in biofilm aggregates and displayed increased tolerance towards antibiotics. In this current study, we aimed at further characterizing the time course of biofilm formation and the impact on antibiotic tolerance development. We found that a S. aureus reference strain, as well as three clinical IE isolates formed biofilms on the IEV after 6h. When treatment was initiated immediately after infection, the antibiotic effect was significantly higher than when treatment was started after the biofilm was allowed to mature. We could follow the biofilm development microscopically by visualizing growing bacterial aggregates on the IEV. The findings indicate that mature, antibiotic-tolerant biofilms can be formed in our model already after 6h, accelerating the screening for optimal treatment strategies for IE.}},
  author       = {{Schwartz, Franziska A. and Nielsen, Luna and Andersen, Jessica Struve and Bock, Magnus and Christophersen, Lars and Sunnerhagen, Torgny and Lerche, Christian Johann and Bay, Lene and Bundgaard, Henning and Høiby, Niels and Moser, Claus}},
  issn         = {{0903-4641}},
  language     = {{eng}},
  number       = {{8}},
  pages        = {{515--523}},
  publisher    = {{John Wiley & Sons Inc.}},
  series       = {{APMIS}},
  title        = {{Dynamics of a Staphylococcus aureus infective endocarditis simulation model}},
  url          = {{https://lup.lub.lu.se/search/files/119180076/APMIS_2022_Schwartz_Dynamics_of_a_Staphylococcus_aureus_infective_endocarditis_simulation_model.pdf}},
  doi          = {{10.1111/apm.13231}},
  volume       = {{130}},
  year         = {{2022}},
}