Purification of F.VIII:C by antigen-antibody chromatography
(1978) In Thrombosis Research 12(4). p.667-675- Abstract
Purification of F.VIII:C devoid of F.VIII:Ag was achieved by antigen-antibody chromatography. The antibody used neutralized VIIIR:Ag but not VIII:C in liquid phase but extracted both VIII:Ag and VIII:C from plasma when bound to Sepharose. VIII:C was eluted with calcium-containing buffer. When plasma was used as starting material VIII:C was obtained free from VIIIR:Ag but contaminated with some other proteins. When a well-defined pure F.VIII preparation was used as starting material the VIII:C active fractions contained no immunoradiometrically detectable VIIIR:Ag, no VIIIR:RCF and no detectable protein. When stabilized with albumin VIII:C could be frozen and thawed with retained activity and could be activated with thrombin.
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https://lup.lub.lu.se/record/98f17493-c0fd-48e8-83e1-0824ada050e2
- author
- Ljung, R. LU and Holmberg, Lars LU
- organization
- publishing date
- 1978
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Thrombosis Research
- volume
- 12
- issue
- 4
- pages
- 9 pages
- publisher
- Elsevier
- external identifiers
-
- pmid:663902
- scopus:0018150726
- ISSN
- 0049-3848
- DOI
- 10.1016/0049-3848(78)90256-6
- language
- English
- LU publication?
- yes
- id
- 98f17493-c0fd-48e8-83e1-0824ada050e2
- date added to LUP
- 2016-10-26 13:52:29
- date last changed
- 2024-01-04 14:59:24
@article{98f17493-c0fd-48e8-83e1-0824ada050e2, abstract = {{<p>Purification of F.VIII:C devoid of F.VIII:Ag was achieved by antigen-antibody chromatography. The antibody used neutralized VIIIR:Ag but not VIII:C in liquid phase but extracted both VIII:Ag and VIII:C from plasma when bound to Sepharose. VIII:C was eluted with calcium-containing buffer. When plasma was used as starting material VIII:C was obtained free from VIIIR:Ag but contaminated with some other proteins. When a well-defined pure F.VIII preparation was used as starting material the VIII:C active fractions contained no immunoradiometrically detectable VIIIR:Ag, no VIIIR:RCF and no detectable protein. When stabilized with albumin VIII:C could be frozen and thawed with retained activity and could be activated with thrombin.</p>}}, author = {{Ljung, R. and Holmberg, Lars}}, issn = {{0049-3848}}, language = {{eng}}, number = {{4}}, pages = {{667--675}}, publisher = {{Elsevier}}, series = {{Thrombosis Research}}, title = {{Purification of F.VIII:C by antigen-antibody chromatography}}, url = {{http://dx.doi.org/10.1016/0049-3848(78)90256-6}}, doi = {{10.1016/0049-3848(78)90256-6}}, volume = {{12}}, year = {{1978}}, }