6;7 chromosomal translocation in spontaneously arising rat immunocytomas : evidence for c-myc breakpoint clustering and correlation between isotypic expression and the c-myc target
(1988) In Molecular and Cellular Biology 8(1). p.51-441- Abstract
Our previous studies have shown that spontaneously arising immunocytomas in the LOU/Ws1 strain of rats contain a t(6;7) chromosomal translocation in all seven tumors studied (F. M. Babonits, J. Spira, G. Klein, and H. Bazin, Int. J. Cancer 29:431-437, 1982). We have also shown that the c-myc is located on chromosome 7 (J. Sümegi, J. Spira, H. Bazin, J. Szpirer, G. Levan, and G. Klein, Nature (London) 306:497-499, 1983) and the immunoglobulin H cluster on chromosome 6 (W.S. Pear, G. Wahlström, J. Szpirer, G. Levan, G. Klein, and J. Sümegi, Immunogenetics 23:393-395, 1986). We now report a detailed cytogenetic and molecular analysis of nine additional rat immunocytomas. The t(6;7) chromosomal translocation is found in all tumors. Mapping... (More)
Our previous studies have shown that spontaneously arising immunocytomas in the LOU/Ws1 strain of rats contain a t(6;7) chromosomal translocation in all seven tumors studied (F. M. Babonits, J. Spira, G. Klein, and H. Bazin, Int. J. Cancer 29:431-437, 1982). We have also shown that the c-myc is located on chromosome 7 (J. Sümegi, J. Spira, H. Bazin, J. Szpirer, G. Levan, and G. Klein, Nature (London) 306:497-499, 1983) and the immunoglobulin H cluster on chromosome 6 (W.S. Pear, G. Wahlström, J. Szpirer, G. Levan, G. Klein, and J. Sümegi, Immunogenetics 23:393-395, 1986). We now report a detailed cytogenetic and molecular analysis of nine additional rat immunocytomas. The t(6;7) chromosomal translocation is found in all tumors. Mapping of the c-myc breakpoints showed that in 10 of 14 tumors, the c-myc breakpoints are clustered in a 1.5-kilobase region upstream of exon 1. In contrast with sporadic Burkitt's lymphoma and mouse plasmacytoma, only 1 of 14 tumors contains the c-myc breakpoints in either exon 1 or intron 1. Analysis of the sequences juxtaposed to the c-myc show that immunoglobulin H switch regions are the targets in at least five tumors and that there is a strong correlation between the secreted immunoglobulin and the c-myc target. Unlike sporadic Burkitt's lymphoma and mouse plasmacytoma, at least two rat immunocytomas show recombination of the c-myc with sequences distinct from immunoglobulin switch regions.
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- author
- Pear, W S ; Wahlström, G ; Nelson, S F ; Axelson, H LU ; Szeles, A ; Wiener, F ; Bazin, H ; Klein, G and Sümegi, J
- publishing date
- 1988-01
- type
- Contribution to journal
- publication status
- published
- keywords
- Animals, Base Sequence, Chromosome Mapping, DNA, Neoplasm, Gene Expression Regulation, Genes, Immunoglobulin, Genes, Switch, Immunoglobulin Constant Regions, Immunoglobulin Heavy Chains, Immunoglobulin Isotypes, Karyotyping, Molecular Sequence Data, Nucleic Acid Hybridization, Plasmacytoma, Proto-Oncogene Proteins, Rats, Translocation, Genetic
- in
- Molecular and Cellular Biology
- volume
- 8
- issue
- 1
- pages
- 11 pages
- publisher
- American Society for Microbiology
- external identifiers
-
- pmid:3122026
- scopus:3543039008
- ISSN
- 0270-7306
- language
- English
- LU publication?
- no
- id
- 99f4ef20-aa85-4330-bbc2-ef3da8ed784c
- date added to LUP
- 2016-08-09 09:21:50
- date last changed
- 2024-01-04 10:35:45
@article{99f4ef20-aa85-4330-bbc2-ef3da8ed784c, abstract = {{<p>Our previous studies have shown that spontaneously arising immunocytomas in the LOU/Ws1 strain of rats contain a t(6;7) chromosomal translocation in all seven tumors studied (F. M. Babonits, J. Spira, G. Klein, and H. Bazin, Int. J. Cancer 29:431-437, 1982). We have also shown that the c-myc is located on chromosome 7 (J. Sümegi, J. Spira, H. Bazin, J. Szpirer, G. Levan, and G. Klein, Nature (London) 306:497-499, 1983) and the immunoglobulin H cluster on chromosome 6 (W.S. Pear, G. Wahlström, J. Szpirer, G. Levan, G. Klein, and J. Sümegi, Immunogenetics 23:393-395, 1986). We now report a detailed cytogenetic and molecular analysis of nine additional rat immunocytomas. The t(6;7) chromosomal translocation is found in all tumors. Mapping of the c-myc breakpoints showed that in 10 of 14 tumors, the c-myc breakpoints are clustered in a 1.5-kilobase region upstream of exon 1. In contrast with sporadic Burkitt's lymphoma and mouse plasmacytoma, only 1 of 14 tumors contains the c-myc breakpoints in either exon 1 or intron 1. Analysis of the sequences juxtaposed to the c-myc show that immunoglobulin H switch regions are the targets in at least five tumors and that there is a strong correlation between the secreted immunoglobulin and the c-myc target. Unlike sporadic Burkitt's lymphoma and mouse plasmacytoma, at least two rat immunocytomas show recombination of the c-myc with sequences distinct from immunoglobulin switch regions.</p>}}, author = {{Pear, W S and Wahlström, G and Nelson, S F and Axelson, H and Szeles, A and Wiener, F and Bazin, H and Klein, G and Sümegi, J}}, issn = {{0270-7306}}, keywords = {{Animals; Base Sequence; Chromosome Mapping; DNA, Neoplasm; Gene Expression Regulation; Genes, Immunoglobulin; Genes, Switch; Immunoglobulin Constant Regions; Immunoglobulin Heavy Chains; Immunoglobulin Isotypes; Karyotyping; Molecular Sequence Data; Nucleic Acid Hybridization; Plasmacytoma; Proto-Oncogene Proteins; Rats; Translocation, Genetic}}, language = {{eng}}, number = {{1}}, pages = {{51--441}}, publisher = {{American Society for Microbiology}}, series = {{Molecular and Cellular Biology}}, title = {{6;7 chromosomal translocation in spontaneously arising rat immunocytomas : evidence for c-myc breakpoint clustering and correlation between isotypic expression and the c-myc target}}, volume = {{8}}, year = {{1988}}, }