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Degradation of [Mercaptopropionic acid1, D‐arginine8] ‐ vasopressin (dDAVP) in Pancreatic Juice and Intestinal Mucosa Homogenate

Lundin, S. ; Bengtsson, H. ‐I ; Folkesson, H. G. LU and Weström, B. R. LU (1989) In Pharmacology & Toxicology 65(2). p.92-95
Abstract

Abstract: The degradation of the vasopressin analogue dDAVP was studied by reversed phase high‐performance liquid chromatography (HPLC) after incubations in pancreatic juice and intestinal mucosa homogenates. dDAVP remained stable in pancreatic juice for a period of 60 min. while the parent hormone arginine vasopressin (AVP) was completely degraded within 5 min. In intestinal mucosa homogenates dDAVP was degraded with half‐lives of 9 min. (fast phase) and 161 min. (slow phase), about four times slower than AVP. By amino‐acid analysis it was confirmed that the metabolite [Mpa1, Des‐D‐Arg8‐Gly9 NH2]‐vasopressin was gradually produced. No other breakdown products were observed. These findings... (More)

Abstract: The degradation of the vasopressin analogue dDAVP was studied by reversed phase high‐performance liquid chromatography (HPLC) after incubations in pancreatic juice and intestinal mucosa homogenates. dDAVP remained stable in pancreatic juice for a period of 60 min. while the parent hormone arginine vasopressin (AVP) was completely degraded within 5 min. In intestinal mucosa homogenates dDAVP was degraded with half‐lives of 9 min. (fast phase) and 161 min. (slow phase), about four times slower than AVP. By amino‐acid analysis it was confirmed that the metabolite [Mpa1, Des‐D‐Arg8‐Gly9 NH2]‐vasopressin was gradually produced. No other breakdown products were observed. These findings may be of value for the further development of more stable peptide analogues which may be effective upon oral administration. 1989 Nordic Pharmacological Society

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author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Pharmacology & Toxicology
volume
65
issue
2
pages
4 pages
publisher
Wiley-Blackwell
external identifiers
  • scopus:0024473378
  • pmid:2813288
ISSN
0901-9928
DOI
10.1111/j.1600-0773.1989.tb01134.x
language
English
LU publication?
yes
id
9ad0543b-f89a-4497-9262-5fbf19c33037
date added to LUP
2024-12-05 15:35:19
date last changed
2025-04-04 14:15:01
@article{9ad0543b-f89a-4497-9262-5fbf19c33037,
  abstract     = {{<p>Abstract:  The degradation of the vasopressin analogue dDAVP was studied by reversed phase high‐performance liquid chromatography (HPLC) after incubations in pancreatic juice and intestinal mucosa homogenates. dDAVP remained stable in pancreatic juice for a period of 60 min. while the parent hormone arginine vasopressin (AVP) was completely degraded within 5 min. In intestinal mucosa homogenates dDAVP was degraded with half‐lives of 9 min. (fast phase) and 161 min. (slow phase), about four times slower than AVP. By amino‐acid analysis it was confirmed that the metabolite [Mpa<sup>1</sup>, Des‐D‐Arg<sup>8</sup>‐Gly<sup>9</sup> NH<sub>2</sub>]‐vasopressin was gradually produced. No other breakdown products were observed. These findings may be of value for the further development of more stable peptide analogues which may be effective upon oral administration. 1989 Nordic Pharmacological Society</p>}},
  author       = {{Lundin, S. and Bengtsson, H. ‐I and Folkesson, H. G. and Weström, B. R.}},
  issn         = {{0901-9928}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{92--95}},
  publisher    = {{Wiley-Blackwell}},
  series       = {{Pharmacology & Toxicology}},
  title        = {{Degradation of [Mercaptopropionic acid<sup>1</sup>, D‐arginine<sup>8</sup>] ‐ vasopressin (dDAVP) in Pancreatic Juice and Intestinal Mucosa Homogenate}},
  url          = {{http://dx.doi.org/10.1111/j.1600-0773.1989.tb01134.x}},
  doi          = {{10.1111/j.1600-0773.1989.tb01134.x}},
  volume       = {{65}},
  year         = {{1989}},
}