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Heterotopic mineral deposits in intact rat Achilles tendons are characterized by a unique fiber-like structure

Pierantoni, Maria LU orcid ; Hammerman, Malin LU ; Silva Barreto, Isabella LU orcid ; Andersson, Linnea ; Novak, Vladimir ; Isaksson, Hanna LU orcid and Eliasson, Pernilla (2023) In Journal of Structural Biology: X 7.
Abstract

Heterotopic mineralization entails pathological mineral formation inside soft tissues. In human tendons mineralization is often associated with tendinopathies, tendon weakness and pain. In Achilles tendons, mineralization is considered to occur through heterotopic ossification (HO) primarily in response to tendon pathologies. However, refined details regarding HO deposition and microstructure are unknown. In this study, we characterize HO in intact rat Achilles tendons through high-resolution phase contrast enhanced synchrotron X-ray tomography. Furthermore, we test the potential of studying local tissue injury by needling intact Achilles tendons and the relation between tissue microdamage and HO. The results show that HO occurs in all... (More)

Heterotopic mineralization entails pathological mineral formation inside soft tissues. In human tendons mineralization is often associated with tendinopathies, tendon weakness and pain. In Achilles tendons, mineralization is considered to occur through heterotopic ossification (HO) primarily in response to tendon pathologies. However, refined details regarding HO deposition and microstructure are unknown. In this study, we characterize HO in intact rat Achilles tendons through high-resolution phase contrast enhanced synchrotron X-ray tomography. Furthermore, we test the potential of studying local tissue injury by needling intact Achilles tendons and the relation between tissue microdamage and HO. The results show that HO occurs in all intact Achilles tendons at 16 weeks of age. HO deposits are characterized by an elongated ellipsoidal shape and by a fiber-like internal structure which suggests that some collagen fibers have mineralized. The data indicates that deposition along fibers initiates in the pericellular area, and propagates into the intercellular area. Within HO deposits cells are larger and more rounded compared to tenocytes between unmineralized fibers, which are fewer and elongated. The results also indicate that multiple HO deposits may merge into bigger structures with time by accession along unmineralized fibers. Furthermore, the presence of unmineralized regions within the deposits may indicate that HOs are not only growing, but mineral resorption may also occur. Additionally, phase contrast synchrotron X-ray tomography allowed to distinguish microdamage at the fiber level in response to needling. The needle injury protocol could in the future enable to elucidate the relation between local inflammation, microdamage, and HO deposition.

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author
; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Calcification, Collagen, Fibers, Pathological mineralization, Phase contrast enhanced synchrotron X-ray tomography
in
Journal of Structural Biology: X
volume
7
article number
100087
publisher
Elsevier
external identifiers
  • scopus:85149679939
  • pmid:36938139
ISSN
2590-1524
DOI
10.1016/j.yjsbx.2023.100087
language
English
LU publication?
yes
id
9c3bca8c-dc25-4388-82ba-d4a27948a430
date added to LUP
2023-04-03 11:30:55
date last changed
2024-06-27 15:22:47
@article{9c3bca8c-dc25-4388-82ba-d4a27948a430,
  abstract     = {{<p>Heterotopic mineralization entails pathological mineral formation inside soft tissues. In human tendons mineralization is often associated with tendinopathies, tendon weakness and pain. In Achilles tendons, mineralization is considered to occur through heterotopic ossification (HO) primarily in response to tendon pathologies. However, refined details regarding HO deposition and microstructure are unknown. In this study, we characterize HO in intact rat Achilles tendons through high-resolution phase contrast enhanced synchrotron X-ray tomography. Furthermore, we test the potential of studying local tissue injury by needling intact Achilles tendons and the relation between tissue microdamage and HO. The results show that HO occurs in all intact Achilles tendons at 16 weeks of age. HO deposits are characterized by an elongated ellipsoidal shape and by a fiber-like internal structure which suggests that some collagen fibers have mineralized. The data indicates that deposition along fibers initiates in the pericellular area, and propagates into the intercellular area. Within HO deposits cells are larger and more rounded compared to tenocytes between unmineralized fibers, which are fewer and elongated. The results also indicate that multiple HO deposits may merge into bigger structures with time by accession along unmineralized fibers. Furthermore, the presence of unmineralized regions within the deposits may indicate that HOs are not only growing, but mineral resorption may also occur. Additionally, phase contrast synchrotron X-ray tomography allowed to distinguish microdamage at the fiber level in response to needling. The needle injury protocol could in the future enable to elucidate the relation between local inflammation, microdamage, and HO deposition.</p>}},
  author       = {{Pierantoni, Maria and Hammerman, Malin and Silva Barreto, Isabella and Andersson, Linnea and Novak, Vladimir and Isaksson, Hanna and Eliasson, Pernilla}},
  issn         = {{2590-1524}},
  keywords     = {{Calcification; Collagen; Fibers; Pathological mineralization; Phase contrast enhanced synchrotron X-ray tomography}},
  language     = {{eng}},
  publisher    = {{Elsevier}},
  series       = {{Journal of Structural Biology: X}},
  title        = {{Heterotopic mineral deposits in intact rat Achilles tendons are characterized by a unique fiber-like structure}},
  url          = {{https://lup.lub.lu.se/search/files/167263348/Pierantoni_JSB_2023.pdf}},
  doi          = {{10.1016/j.yjsbx.2023.100087}},
  volume       = {{7}},
  year         = {{2023}},
}