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Allergen-specific human IgE isolated through an allergen-agnostic pipeline—understanding immune response and allergen recognition

Thörnqvist, Linnea LU ; Franciskovic, Eric LU ; Godzwon, Magdalena LU orcid ; Kristensen, Bjarne ; Sultan, Kristin ; Nordström, Franziska LU orcid ; Palmason, Robert LU ; Todorovic, Nikolina ; Keller, Walter and Lindstedt, Malin LU orcid , et al. (2026) In Communications Biology 9(1).
Abstract

Allergy, characterised by antibody responses of the IgE isotype, is a major health concern. The set of monoclonal human IgE used for studying the molecular mechanisms of allergies is limited. Single-cell sequencing offers opportunities to establish novel antibodies for researching, diagnosis, and treatment of allergies. We describe and exploit a pipeline for generating recombinant IgE directly from the immune repertoires of allergic subjects. It uses single-cell sequencing of IgM– B cells of bone marrow and peripheral blood in an allergen-agnostic manner, combined with high-throughput transcriptome sequencing to identify clonotypes populating the IgE repertoire. Immunochemical and immunoprecipitation analyses are used to... (More)

Allergy, characterised by antibody responses of the IgE isotype, is a major health concern. The set of monoclonal human IgE used for studying the molecular mechanisms of allergies is limited. Single-cell sequencing offers opportunities to establish novel antibodies for researching, diagnosis, and treatment of allergies. We describe and exploit a pipeline for generating recombinant IgE directly from the immune repertoires of allergic subjects. It uses single-cell sequencing of IgM– B cells of bone marrow and peripheral blood in an allergen-agnostic manner, combined with high-throughput transcriptome sequencing to identify clonotypes populating the IgE repertoire. Immunochemical and immunoprecipitation analyses are used to deconvolute the specificity of identified antibodies. High-affinity antibodies were raised against four grass pollen allergens, antibodies that illustrated aspects of the development of allergen-specific humoral immunity. The pipeline provides a streamlined approach for the development and characterisation of native allergen-specific antibodies as they occur in allergy and during allergy desensitisation.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Communications Biology
volume
9
issue
1
article number
332
publisher
Nature Publishing Group
external identifiers
  • pmid:41606257
  • scopus:105031602346
ISSN
2399-3642
DOI
10.1038/s42003-026-09600-3
language
English
LU publication?
yes
id
9d9a9107-05b0-4986-95e3-694999648d80
date added to LUP
2026-03-26 12:37:30
date last changed
2026-03-27 03:00:02
@article{9d9a9107-05b0-4986-95e3-694999648d80,
  abstract     = {{<p>Allergy, characterised by antibody responses of the IgE isotype, is a major health concern. The set of monoclonal human IgE used for studying the molecular mechanisms of allergies is limited. Single-cell sequencing offers opportunities to establish novel antibodies for researching, diagnosis, and treatment of allergies. We describe and exploit a pipeline for generating recombinant IgE directly from the immune repertoires of allergic subjects. It uses single-cell sequencing of IgM<sup>–</sup> B cells of bone marrow and peripheral blood in an allergen-agnostic manner, combined with high-throughput transcriptome sequencing to identify clonotypes populating the IgE repertoire. Immunochemical and immunoprecipitation analyses are used to deconvolute the specificity of identified antibodies. High-affinity antibodies were raised against four grass pollen allergens, antibodies that illustrated aspects of the development of allergen-specific humoral immunity. The pipeline provides a streamlined approach for the development and characterisation of native allergen-specific antibodies as they occur in allergy and during allergy desensitisation.</p>}},
  author       = {{Thörnqvist, Linnea and Franciskovic, Eric and Godzwon, Magdalena and Kristensen, Bjarne and Sultan, Kristin and Nordström, Franziska and Palmason, Robert and Todorovic, Nikolina and Keller, Walter and Lindstedt, Malin and Greiff, Lennart and Levander, Fredrik and Ohlin, Mats}},
  issn         = {{2399-3642}},
  language     = {{eng}},
  number       = {{1}},
  publisher    = {{Nature Publishing Group}},
  series       = {{Communications Biology}},
  title        = {{Allergen-specific human IgE isolated through an allergen-agnostic pipeline—understanding immune response and allergen recognition}},
  url          = {{http://dx.doi.org/10.1038/s42003-026-09600-3}},
  doi          = {{10.1038/s42003-026-09600-3}},
  volume       = {{9}},
  year         = {{2026}},
}