Development of a Sol-gel-assisted Reverse-phase Microarray Platform for Simple and Rapid Detection of Prostate-specific Antigen from Serum
(2018) In Biochip Journal 12(1). p.69-74- Abstract
A sol-gel-based reverse-phase microarray was developed with improved sensitivity for prostatespecific antigen (PSA) from serum. The pore-sizecontrolled 3D sol-gel matrix was created with a large surface area to capture target molecules densely. Using the optically active sol-gel nanocomposites, human female serum was spiked with PSA and assessed using the reverse-phase protein microarray. The reverse-phase assay exhibited a limit of detection of 1 pg/mL for PSA and a dynamic range of 103 orders of magnitude. Notably, the platform matched the demand of the immunoassay in a simple and feasible manner. Moreover, the platform shortened the total assay time with an increased accuracy of diagnosis.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/a884ddc0-2b18-414a-8797-3cdb79e134f6
- author
- Lee, Sang Wook LU ; Laurell, Thomas LU ; Jeong, Ok Chan and Kim, Soyoun
- organization
- publishing date
- 2018-03-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Macro/nano-structure silicon, Prostate cancer, Prostate-specific antigen, Reverse-phase assay, Sol-gel microarray
- in
- Biochip Journal
- volume
- 12
- issue
- 1
- pages
- 6 pages
- publisher
- Korean BioChip Society (KBCS)
- external identifiers
-
- scopus:85044193601
- ISSN
- 1976-0280
- DOI
- 10.1007/s13206-017-2109-8
- language
- English
- LU publication?
- yes
- id
- a884ddc0-2b18-414a-8797-3cdb79e134f6
- date added to LUP
- 2018-04-05 14:50:29
- date last changed
- 2022-05-10 22:57:06
@article{a884ddc0-2b18-414a-8797-3cdb79e134f6, abstract = {{<p>A sol-gel-based reverse-phase microarray was developed with improved sensitivity for prostatespecific antigen (PSA) from serum. The pore-sizecontrolled 3D sol-gel matrix was created with a large surface area to capture target molecules densely. Using the optically active sol-gel nanocomposites, human female serum was spiked with PSA and assessed using the reverse-phase protein microarray. The reverse-phase assay exhibited a limit of detection of 1 pg/mL for PSA and a dynamic range of 10<sup>3</sup> orders of magnitude. Notably, the platform matched the demand of the immunoassay in a simple and feasible manner. Moreover, the platform shortened the total assay time with an increased accuracy of diagnosis.</p>}}, author = {{Lee, Sang Wook and Laurell, Thomas and Jeong, Ok Chan and Kim, Soyoun}}, issn = {{1976-0280}}, keywords = {{Macro/nano-structure silicon; Prostate cancer; Prostate-specific antigen; Reverse-phase assay; Sol-gel microarray}}, language = {{eng}}, month = {{03}}, number = {{1}}, pages = {{69--74}}, publisher = {{Korean BioChip Society (KBCS)}}, series = {{Biochip Journal}}, title = {{Development of a Sol-gel-assisted Reverse-phase Microarray Platform for Simple and Rapid Detection of Prostate-specific Antigen from Serum}}, url = {{http://dx.doi.org/10.1007/s13206-017-2109-8}}, doi = {{10.1007/s13206-017-2109-8}}, volume = {{12}}, year = {{2018}}, }