Abnormal cell cycle regulation in malignancy
(1999) In American Journal of Clinical Pathology 112(1 Suppl 1). p.40-52- Abstract
The cell cycle consists of an initial growth phase (G1), DNA replication (S), a gap phase (G2), and mitosis (M), after which the cell may differentiate or enter the resting state (G0). The cycle is driven by a number of positive and negative regulatory phosphorylation and dephosphorylation events, involving protein kinases, protein phosphatases, cyclins, cyclin-dependent kinases, and cyclin-dependent kinase inhibitors, that ultimately impinge on the activity of transcription factors. Unreplicated or damaged DNA blocks the progression of the cell cycle at checkpoints, including a late G1 checkpoint regulated by the dephosphorylated retinoblastoma protein and a late G2 checkpoint regulated by the phosphorylation of cyclin-dependent kinase... (More)
The cell cycle consists of an initial growth phase (G1), DNA replication (S), a gap phase (G2), and mitosis (M), after which the cell may differentiate or enter the resting state (G0). The cycle is driven by a number of positive and negative regulatory phosphorylation and dephosphorylation events, involving protein kinases, protein phosphatases, cyclins, cyclin-dependent kinases, and cyclin-dependent kinase inhibitors, that ultimately impinge on the activity of transcription factors. Unreplicated or damaged DNA blocks the progression of the cell cycle at checkpoints, including a late G1 checkpoint regulated by the dephosphorylated retinoblastoma protein and a late G2 checkpoint regulated by the phosphorylation of cyclin-dependent kinase 1 complexed with cyclin B. Many cell cycle regulator genes may be considered proto-oncogenes or tumor suppressor genes, and point mutations, amplifications, deletions, or rearrangements involving their loci, particularly those in the "RB pathway," are associated with various tumors. A number of molecular techniques may be used to detect genomic alterations or posttranscriptional modifications, but immunohistochemistry remains the most common method to determine expression levels of a regulatory protein. Multivariate analysis of the usefulness in prognosis has been applied most often for the general proliferation antigen Ki-67.
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- author
- Dictor, Michael LU ; Ehinger, Mats LU ; Mertens, Fredrik LU ; Åkervall, Jan LU and Wennerberg, Johan LU
- organization
- publishing date
- 1999-07
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Biomarkers, Tumor/genetics, Cell Cycle, Cell Cycle Proteins/genetics, Cost-Benefit Analysis, DNA, Neoplasm/analysis, Genetic Techniques/economics, Humans, Neoplasms/metabolism
- in
- American Journal of Clinical Pathology
- volume
- 112
- issue
- 1 Suppl 1
- pages
- 13 pages
- publisher
- Oxford University Press
- external identifiers
-
- scopus:0033506770
- pmid:10396300
- ISSN
- 0002-9173
- language
- English
- LU publication?
- yes
- id
- ab8d7b61-f00b-4711-a84a-ba5d2c13f09f
- date added to LUP
- 2022-01-23 15:32:03
- date last changed
- 2024-04-20 20:09:41
@article{ab8d7b61-f00b-4711-a84a-ba5d2c13f09f, abstract = {{<p>The cell cycle consists of an initial growth phase (G1), DNA replication (S), a gap phase (G2), and mitosis (M), after which the cell may differentiate or enter the resting state (G0). The cycle is driven by a number of positive and negative regulatory phosphorylation and dephosphorylation events, involving protein kinases, protein phosphatases, cyclins, cyclin-dependent kinases, and cyclin-dependent kinase inhibitors, that ultimately impinge on the activity of transcription factors. Unreplicated or damaged DNA blocks the progression of the cell cycle at checkpoints, including a late G1 checkpoint regulated by the dephosphorylated retinoblastoma protein and a late G2 checkpoint regulated by the phosphorylation of cyclin-dependent kinase 1 complexed with cyclin B. Many cell cycle regulator genes may be considered proto-oncogenes or tumor suppressor genes, and point mutations, amplifications, deletions, or rearrangements involving their loci, particularly those in the "RB pathway," are associated with various tumors. A number of molecular techniques may be used to detect genomic alterations or posttranscriptional modifications, but immunohistochemistry remains the most common method to determine expression levels of a regulatory protein. Multivariate analysis of the usefulness in prognosis has been applied most often for the general proliferation antigen Ki-67.</p>}}, author = {{Dictor, Michael and Ehinger, Mats and Mertens, Fredrik and Åkervall, Jan and Wennerberg, Johan}}, issn = {{0002-9173}}, keywords = {{Biomarkers, Tumor/genetics; Cell Cycle; Cell Cycle Proteins/genetics; Cost-Benefit Analysis; DNA, Neoplasm/analysis; Genetic Techniques/economics; Humans; Neoplasms/metabolism}}, language = {{eng}}, number = {{1 Suppl 1}}, pages = {{40--52}}, publisher = {{Oxford University Press}}, series = {{American Journal of Clinical Pathology}}, title = {{Abnormal cell cycle regulation in malignancy}}, volume = {{112}}, year = {{1999}}, }