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Retinal regions shape human and murine Müller cell proteome profile and functionality

Kaplan, Lew ; Drexler, Corinne ; Pfaller, Anna M. ; Brenna, Santra ; Wunderlich, Kirsten A. LU ; Dimitracopoulos, Andrea ; Merl-Pham, Juliane ; Perez, Maria Theresa LU ; Schlötzer-Schrehardt, Ursula and Enzmann, Volker , et al. (2023) In GLIA 71(2). p.391-414
Abstract

The human macula is a highly specialized retinal region with pit-like morphology and rich in cones. How Müller cells, the principal glial cell type in the retina, are adapted to this environment is still poorly understood. We compared proteomic data from cone- and rod-rich retinae from human and mice and identified different expression profiles of cone- and rod-associated Müller cells that converged on pathways representing extracellular matrix and cell adhesion. In particular, epiplakin (EPPK1), which is thought to play a role in intermediate filament organization, was highly expressed in macular Müller cells. Furthermore, EPPK1 knockout in a human Müller cell-derived cell line led to a decrease in traction forces as well as to changes... (More)

The human macula is a highly specialized retinal region with pit-like morphology and rich in cones. How Müller cells, the principal glial cell type in the retina, are adapted to this environment is still poorly understood. We compared proteomic data from cone- and rod-rich retinae from human and mice and identified different expression profiles of cone- and rod-associated Müller cells that converged on pathways representing extracellular matrix and cell adhesion. In particular, epiplakin (EPPK1), which is thought to play a role in intermediate filament organization, was highly expressed in macular Müller cells. Furthermore, EPPK1 knockout in a human Müller cell-derived cell line led to a decrease in traction forces as well as to changes in cell size, shape, and filopodia characteristics. We here identified EPPK1 as a central molecular player in the region-specific architecture of the human retina, which likely enables specific functions under the immense mechanical loads in vivo.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
EPPK1, glial heterogeneity, macula, Müller cells, retina
in
GLIA
volume
71
issue
2
pages
391 - 414
publisher
John Wiley & Sons Inc.
external identifiers
  • scopus:85141475483
  • pmid:36334068
ISSN
0894-1491
DOI
10.1002/glia.24283
language
English
LU publication?
yes
id
ace12392-ecfb-408d-b7ac-e1018fc50715
date added to LUP
2022-12-20 16:27:07
date last changed
2024-06-13 23:59:49
@article{ace12392-ecfb-408d-b7ac-e1018fc50715,
  abstract     = {{<p>The human macula is a highly specialized retinal region with pit-like morphology and rich in cones. How Müller cells, the principal glial cell type in the retina, are adapted to this environment is still poorly understood. We compared proteomic data from cone- and rod-rich retinae from human and mice and identified different expression profiles of cone- and rod-associated Müller cells that converged on pathways representing extracellular matrix and cell adhesion. In particular, epiplakin (EPPK1), which is thought to play a role in intermediate filament organization, was highly expressed in macular Müller cells. Furthermore, EPPK1 knockout in a human Müller cell-derived cell line led to a decrease in traction forces as well as to changes in cell size, shape, and filopodia characteristics. We here identified EPPK1 as a central molecular player in the region-specific architecture of the human retina, which likely enables specific functions under the immense mechanical loads in vivo.</p>}},
  author       = {{Kaplan, Lew and Drexler, Corinne and Pfaller, Anna M. and Brenna, Santra and Wunderlich, Kirsten A. and Dimitracopoulos, Andrea and Merl-Pham, Juliane and Perez, Maria Theresa and Schlötzer-Schrehardt, Ursula and Enzmann, Volker and Samardzija, Marijana and Puig, Berta and Fuchs, Peter and Franze, Kristian and Hauck, Stefanie M. and Grosche, Antje}},
  issn         = {{0894-1491}},
  keywords     = {{EPPK1; glial heterogeneity; macula; Müller cells; retina}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{391--414}},
  publisher    = {{John Wiley & Sons Inc.}},
  series       = {{GLIA}},
  title        = {{Retinal regions shape human and murine Müller cell proteome profile and functionality}},
  url          = {{http://dx.doi.org/10.1002/glia.24283}},
  doi          = {{10.1002/glia.24283}},
  volume       = {{71}},
  year         = {{2023}},
}