Production and HPLC-Based Disaccharide Analysis of Xyloside-Primed Glycosaminoglycans
(2022) In Methods in Molecular Biology 2303. p.173-182- Abstract
Although glycosaminoglycans (GAGs) are known to be involved in a variety of physiological and pathological processes, knowledge about their expression by cells or tissues, the GAGome, is limited. Xylosides can be used to induce the formation of GAGs without the presence of a proteoglycan core protein. The administration of xylosides to living cells tends to result in a considerable amplification in GAG production, and the xylosides can, therefore, be used as analytical tools to study the GAG produced by a certain cell type. One of the most common ways to analyze the GAGs structurally is by disaccharide analysis, which involves depolymerization of the GAGs into disaccharides, fluorescent labeling of the disaccharides with... (More)
Although glycosaminoglycans (GAGs) are known to be involved in a variety of physiological and pathological processes, knowledge about their expression by cells or tissues, the GAGome, is limited. Xylosides can be used to induce the formation of GAGs without the presence of a proteoglycan core protein. The administration of xylosides to living cells tends to result in a considerable amplification in GAG production, and the xylosides can, therefore, be used as analytical tools to study the GAG produced by a certain cell type. One of the most common ways to analyze the GAGs structurally is by disaccharide analysis, which involves depolymerization of the GAGs into disaccharides, fluorescent labeling of the disaccharides with 2-aminoacridone, and quantification using high-pressure liquid chromatography (HPLC). Here, we describe the procedure of producing xyloside-primed GAGs and how to study them structurally by disaccharide analysis.
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- author
- Persson, Andrea ; Tykesson, Emil LU ; Ellervik, Ulf LU and Mani, Katrin LU
- organization
- publishing date
- 2022
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- keywords
- Chondroitin sulfate, Chondroitinase, Dermatan sulfate, Glycosaminoglycan, Heparan sulfate, Heparinase, High-pressure liquid chromatography (HPLC), Proteoglycan, Structural analysis, Xyloside
- host publication
- Methods in Molecular Biology
- series title
- Methods in Molecular Biology
- volume
- 2303
- pages
- 10 pages
- publisher
- Humana Press
- external identifiers
-
- scopus:85117104221
- pmid:34626379
- ISSN
- 1064-3745
- 1940-6029
- DOI
- 10.1007/978-1-0716-1398-6_15
- language
- English
- LU publication?
- yes
- additional info
- Publisher Copyright: © 2022, Springer Science+Business Media, LLC, part of Springer Nature.
- id
- b73947bb-ba9c-4169-84b3-633297ff37d2
- date added to LUP
- 2021-11-01 13:49:53
- date last changed
- 2024-08-11 00:30:51
@inbook{b73947bb-ba9c-4169-84b3-633297ff37d2, abstract = {{<p>Although glycosaminoglycans (GAGs) are known to be involved in a variety of physiological and pathological processes, knowledge about their expression by cells or tissues, the GAGome, is limited. Xylosides can be used to induce the formation of GAGs without the presence of a proteoglycan core protein. The administration of xylosides to living cells tends to result in a considerable amplification in GAG production, and the xylosides can, therefore, be used as analytical tools to study the GAG produced by a certain cell type. One of the most common ways to analyze the GAGs structurally is by disaccharide analysis, which involves depolymerization of the GAGs into disaccharides, fluorescent labeling of the disaccharides with 2-aminoacridone, and quantification using high-pressure liquid chromatography (HPLC). Here, we describe the procedure of producing xyloside-primed GAGs and how to study them structurally by disaccharide analysis.</p>}}, author = {{Persson, Andrea and Tykesson, Emil and Ellervik, Ulf and Mani, Katrin}}, booktitle = {{Methods in Molecular Biology}}, issn = {{1064-3745}}, keywords = {{Chondroitin sulfate; Chondroitinase; Dermatan sulfate; Glycosaminoglycan; Heparan sulfate; Heparinase; High-pressure liquid chromatography (HPLC); Proteoglycan; Structural analysis; Xyloside}}, language = {{eng}}, pages = {{173--182}}, publisher = {{Humana Press}}, series = {{Methods in Molecular Biology}}, title = {{Production and HPLC-Based Disaccharide Analysis of Xyloside-Primed Glycosaminoglycans}}, url = {{http://dx.doi.org/10.1007/978-1-0716-1398-6_15}}, doi = {{10.1007/978-1-0716-1398-6_15}}, volume = {{2303}}, year = {{2022}}, }