Straightforward purification method for the determination of the activity of glucose oxidase and catalase in honey by extracting polyphenols with a film-shaped polymer
(2023) In Food Chemistry 405.- Abstract
Glucose oxidase (GOX) and catalase (CAT) regulate the amount of H2O2 in honey, by generating or consuming it, so they are related to the antibacterial and antioxidant activity of honey. However, their activities are hardly analysed, since the process requires a previous dialysis that is non-selective, very time-consuming (>24 h), eco-unfriendly (>6L of buffer) and expensive. This research shows the design and performance of a material that selectively removes the actual interferents. The film-shaped-polymer is immersed for 90́ within a honey solution (12.5 mL of buffer), where it interacts exclusively with 1,2-dihydroxybenzenes, which we proved to be the real interferents (the material contains motifs derived from phenylboronic... (More)
Glucose oxidase (GOX) and catalase (CAT) regulate the amount of H2O2 in honey, by generating or consuming it, so they are related to the antibacterial and antioxidant activity of honey. However, their activities are hardly analysed, since the process requires a previous dialysis that is non-selective, very time-consuming (>24 h), eco-unfriendly (>6L of buffer) and expensive. This research shows the design and performance of a material that selectively removes the actual interferents. The film-shaped-polymer is immersed for 90́ within a honey solution (12.5 mL of buffer), where it interacts exclusively with 1,2-dihydroxybenzenes, which we proved to be the real interferents (the material contains motifs derived from phenylboronic acid to interact with 1,2-diols). Polymeric chains favour condensation to occur exclusively with 1,2-dihydroxybenzenes, excluding monosaccharides. The interferents’ removal using our designed polymer is selective, low cost (1.42€ per test), rapid and eco-friendly (saves 6L of buffer and 20.5 h of experimental workout per sample).
(Less)
- author
- González-Ceballos, Lara
; Carlos Guirado-Moreno, Jose
; Utzeri, Gianluca
LU
; Miguel García, José ; Fernández-Muiño, Miguel A. ; Osés, Sandra M. ; Teresa Sancho, M. ; Arnaiz, Ana ; Valente, Artur J.M. and Vallejos, Saúl
- publishing date
- 2023-03-30
- type
- Contribution to journal
- publication status
- published
- keywords
- 1,2-Dihydroxybenzene, Catalase, Catechol, Glucose oxidase, Honey, Phenylboronic acid
- in
- Food Chemistry
- volume
- 405
- article number
- 134789
- publisher
- Elsevier
- external identifiers
-
- pmid:36347201
- scopus:85141224639
- ISSN
- 0308-8146
- DOI
- 10.1016/j.foodchem.2022.134789
- language
- English
- LU publication?
- no
- additional info
- Publisher Copyright: © 2022 The Author(s)
- id
- c0f60517-99c4-4392-9930-34cff4f1a9b9
- date added to LUP
- 2024-07-22 10:49:27
- date last changed
- 2024-08-15 10:00:43
@article{c0f60517-99c4-4392-9930-34cff4f1a9b9, abstract = {{<p>Glucose oxidase (GOX) and catalase (CAT) regulate the amount of H2O2 in honey, by generating or consuming it, so they are related to the antibacterial and antioxidant activity of honey. However, their activities are hardly analysed, since the process requires a previous dialysis that is non-selective, very time-consuming (>24 h), eco-unfriendly (>6L of buffer) and expensive. This research shows the design and performance of a material that selectively removes the actual interferents. The film-shaped-polymer is immersed for 90́ within a honey solution (12.5 mL of buffer), where it interacts exclusively with 1,2-dihydroxybenzenes, which we proved to be the real interferents (the material contains motifs derived from phenylboronic acid to interact with 1,2-diols). Polymeric chains favour condensation to occur exclusively with 1,2-dihydroxybenzenes, excluding monosaccharides. The interferents’ removal using our designed polymer is selective, low cost (1.42€ per test), rapid and eco-friendly (saves 6L of buffer and 20.5 h of experimental workout per sample).</p>}}, author = {{González-Ceballos, Lara and Carlos Guirado-Moreno, Jose and Utzeri, Gianluca and Miguel García, José and Fernández-Muiño, Miguel A. and Osés, Sandra M. and Teresa Sancho, M. and Arnaiz, Ana and Valente, Artur J.M. and Vallejos, Saúl}}, issn = {{0308-8146}}, keywords = {{1,2-Dihydroxybenzene; Catalase; Catechol; Glucose oxidase; Honey; Phenylboronic acid}}, language = {{eng}}, month = {{03}}, publisher = {{Elsevier}}, series = {{Food Chemistry}}, title = {{Straightforward purification method for the determination of the activity of glucose oxidase and catalase in honey by extracting polyphenols with a film-shaped polymer}}, url = {{http://dx.doi.org/10.1016/j.foodchem.2022.134789}}, doi = {{10.1016/j.foodchem.2022.134789}}, volume = {{405}}, year = {{2023}}, }