Use of celite-immobilised chloroperoxidase in predominantly organic media
(1999) In Biocatalysis and Biotransformation 17(4). p.293-303- Abstract
It is possible to use the chloroperoxidase from Caldariomyces fumago to catalyse the oxidation of benzyl alcohol to benzaldehyde in predominantly organic media, even at water activities below 1.0, if the oxidant is tert-butyl hydroperoxide. The enzyme activity increased with increasing water activity and no enzyme activity was observed at water activities below 0.6. Hydrophobic solvents such as cyclohexane showed the highest initial activities. It was beneficial in terms of obtainable yield to use high concentrations of the peroxide due to the kinetics of the system. The apparent K(m) for benzyl alcohol in cyclohexane was estimated to be 13 mM while the initial reaction rate increased almost linearly up to 250 mM tert-butyl... (More)
It is possible to use the chloroperoxidase from Caldariomyces fumago to catalyse the oxidation of benzyl alcohol to benzaldehyde in predominantly organic media, even at water activities below 1.0, if the oxidant is tert-butyl hydroperoxide. The enzyme activity increased with increasing water activity and no enzyme activity was observed at water activities below 0.6. Hydrophobic solvents such as cyclohexane showed the highest initial activities. It was beneficial in terms of obtainable yield to use high concentrations of the peroxide due to the kinetics of the system. The apparent K(m) for benzyl alcohol in cyclohexane was estimated to be 13 mM while the initial reaction rate increased almost linearly up to 250 mM tert-butyl hydroperoxide.
(Less)
- author
- Andersson, Mats LU ; Samra, Babinder K. ; Holmberg, Hans and Adlercreutz, Patrick LU
- organization
- publishing date
- 1999-01-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Alcohol oxidation, CPO, Organic solvents
- in
- Biocatalysis and Biotransformation
- volume
- 17
- issue
- 4
- pages
- 11 pages
- publisher
- Taylor & Francis
- external identifiers
-
- scopus:0032586515
- ISSN
- 1024-2422
- DOI
- 10.3109/10242429909015232
- language
- English
- LU publication?
- yes
- id
- d1c0e369-adb3-43b0-8625-e4a993b1d48d
- date added to LUP
- 2019-06-20 15:36:00
- date last changed
- 2022-01-31 22:13:21
@article{d1c0e369-adb3-43b0-8625-e4a993b1d48d, abstract = {{<p>It is possible to use the chloroperoxidase from Caldariomyces fumago to catalyse the oxidation of benzyl alcohol to benzaldehyde in predominantly organic media, even at water activities below 1.0, if the oxidant is tert-butyl hydroperoxide. The enzyme activity increased with increasing water activity and no enzyme activity was observed at water activities below 0.6. Hydrophobic solvents such as cyclohexane showed the highest initial activities. It was beneficial in terms of obtainable yield to use high concentrations of the peroxide due to the kinetics of the system. The apparent K(m) for benzyl alcohol in cyclohexane was estimated to be 13 mM while the initial reaction rate increased almost linearly up to 250 mM tert-butyl hydroperoxide.</p>}}, author = {{Andersson, Mats and Samra, Babinder K. and Holmberg, Hans and Adlercreutz, Patrick}}, issn = {{1024-2422}}, keywords = {{Alcohol oxidation; CPO; Organic solvents}}, language = {{eng}}, month = {{01}}, number = {{4}}, pages = {{293--303}}, publisher = {{Taylor & Francis}}, series = {{Biocatalysis and Biotransformation}}, title = {{Use of celite-immobilised chloroperoxidase in predominantly organic media}}, url = {{http://dx.doi.org/10.3109/10242429909015232}}, doi = {{10.3109/10242429909015232}}, volume = {{17}}, year = {{1999}}, }