Polymorphism in killer cell immunoglobulin-like receptors and human leukocyte antigen-c and predisposition to preeclampsia in Ethiopian pregnant women population
(2020) In Journal of Reproductive Immunology 141.- Abstract
Introduction: Preeclampsia (PE) is a human specific pregnancy-related syndrome of unknown etiology that affects 2–8 % of pregnancies. Polymorphism in maternal Killer Cell Immunoglobulin-like Receptors (KIRs) and the ligand fetal Human Leukocyte Antigen-C (HLA-C) may predispose pregnant mothers for PE due to defective trophoblast invasion into the maternal decidua. Our study aimed to investigate the association between maternal KIR and fetal HLA-C polymorphism and PE in Ethiopian pregnant women. Methods: We included a total of 288 (157 controls and 131 PE cases) in a case-controls study at Adama Regional Referral Hospital, Ethiopia. The KIR and HLA-C genotyping was done using traditional polymerase chain reaction on genomic DNA extracted... (More)
Introduction: Preeclampsia (PE) is a human specific pregnancy-related syndrome of unknown etiology that affects 2–8 % of pregnancies. Polymorphism in maternal Killer Cell Immunoglobulin-like Receptors (KIRs) and the ligand fetal Human Leukocyte Antigen-C (HLA-C) may predispose pregnant mothers for PE due to defective trophoblast invasion into the maternal decidua. Our study aimed to investigate the association between maternal KIR and fetal HLA-C polymorphism and PE in Ethiopian pregnant women. Methods: We included a total of 288 (157 controls and 131 PE cases) in a case-controls study at Adama Regional Referral Hospital, Ethiopia. The KIR and HLA-C genotyping was done using traditional polymerase chain reaction on genomic DNA extracted form maternal venous and cord blood followed by 2% agarose gel electrophoresis. Results: The statistical associations between variables were evaluated using Pearson's Chi-square test. P < 0.05, with 95 % confidence interval was considered statistically significant. A significant association was observed between the KIR2DS1 and PE, with a higher frequency (60.5 %) of the gene in the control group. Similarly, a significant association was observed between KIR AA genotype and PE, with a higher frequency (38.2 %) of this genotype in the PE group. Ethiopians share the same risk genotype for PE as seen in previous African and European studies, namely homozygosity of a maternal KIR AA genotype. However, Ethiopians differ from other East African populations by sharing the same protective KIR2DS1 gene as Europeans.
(Less)
- author
- Kelemu, Tsehayneh LU ; Erlandsson, Lena LU ; Seifu, Daniel ; Hansson, Eva LU ; Abebe, Markos ; Teklu, Sisay ; Girma, Selfu ; Traherne, James A. ; Moffett, Ashley and Hansson, Stefan R. LU
- organization
- publishing date
- 2020
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- HLA, KIR, Natural killer cell, Preeclampsia, Trophoblast cell
- in
- Journal of Reproductive Immunology
- volume
- 141
- article number
- 103169
- publisher
- Elsevier
- external identifiers
-
- pmid:32603992
- scopus:85086881824
- ISSN
- 0165-0378
- DOI
- 10.1016/j.jri.2020.103169
- language
- English
- LU publication?
- yes
- id
- d2246132-7032-4d13-b9c2-945417051ca4
- date added to LUP
- 2020-07-07 10:01:34
- date last changed
- 2024-09-19 02:00:33
@article{d2246132-7032-4d13-b9c2-945417051ca4, abstract = {{<p>Introduction: Preeclampsia (PE) is a human specific pregnancy-related syndrome of unknown etiology that affects 2–8 % of pregnancies. Polymorphism in maternal Killer Cell Immunoglobulin-like Receptors (KIRs) and the ligand fetal Human Leukocyte Antigen-C (HLA-C) may predispose pregnant mothers for PE due to defective trophoblast invasion into the maternal decidua. Our study aimed to investigate the association between maternal KIR and fetal HLA-C polymorphism and PE in Ethiopian pregnant women. Methods: We included a total of 288 (157 controls and 131 PE cases) in a case-controls study at Adama Regional Referral Hospital, Ethiopia. The KIR and HLA-C genotyping was done using traditional polymerase chain reaction on genomic DNA extracted form maternal venous and cord blood followed by 2% agarose gel electrophoresis. Results: The statistical associations between variables were evaluated using Pearson's Chi-square test. P < 0.05, with 95 % confidence interval was considered statistically significant. A significant association was observed between the KIR2DS1 and PE, with a higher frequency (60.5 %) of the gene in the control group. Similarly, a significant association was observed between KIR AA genotype and PE, with a higher frequency (38.2 %) of this genotype in the PE group. Ethiopians share the same risk genotype for PE as seen in previous African and European studies, namely homozygosity of a maternal KIR AA genotype. However, Ethiopians differ from other East African populations by sharing the same protective KIR2DS1 gene as Europeans.</p>}}, author = {{Kelemu, Tsehayneh and Erlandsson, Lena and Seifu, Daniel and Hansson, Eva and Abebe, Markos and Teklu, Sisay and Girma, Selfu and Traherne, James A. and Moffett, Ashley and Hansson, Stefan R.}}, issn = {{0165-0378}}, keywords = {{HLA; KIR; Natural killer cell; Preeclampsia; Trophoblast cell}}, language = {{eng}}, publisher = {{Elsevier}}, series = {{Journal of Reproductive Immunology}}, title = {{Polymorphism in killer cell immunoglobulin-like receptors and human leukocyte antigen-c and predisposition to preeclampsia in Ethiopian pregnant women population}}, url = {{http://dx.doi.org/10.1016/j.jri.2020.103169}}, doi = {{10.1016/j.jri.2020.103169}}, volume = {{141}}, year = {{2020}}, }