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Determining platelet activation and aggregation in response to bacteria

Shannon, Oonagh LU (2017) In Methods in Molecular Biology 1535. p.267-273
Abstract

Many pathogenic bacteria have been reported to interact with human platelets to mediate platelet activation and aggregation. The importance of these interactions to the immune response or pathogenesis of bacterial infection has not been clarified. It may therefore be valuable to assess platelet responses mediated by diverse strains of bacteria. Here, I describe a method to study platelet integrin activation and granule release using flow cytometry, and a complementary method to study platelet aggregation using a dedicated platelet aggregometer. The combination of these methods represents a rapid and cost-effective strategy to provide mechanistic insight on the type of platelet response mediated by the bacteria.

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author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
Bacteria, Coagulation, Flow cytometry, Platelets, Streptococci
host publication
Methods in Molecular Biology
series title
Methods in Molecular Biology
volume
1535
pages
7 pages
publisher
Humana Press
external identifiers
  • pmid:27914085
  • scopus:85006001811
ISSN
10643745
DOI
10.1007/978-1-4939-6673-8_17
language
English
LU publication?
yes
id
d2d8fceb-9b60-4916-ad95-3b1f6f9113f7
date added to LUP
2017-03-16 13:50:58
date last changed
2025-01-07 09:54:13
@inbook{d2d8fceb-9b60-4916-ad95-3b1f6f9113f7,
  abstract     = {{<p>Many pathogenic bacteria have been reported to interact with human platelets to mediate platelet activation and aggregation. The importance of these interactions to the immune response or pathogenesis of bacterial infection has not been clarified. It may therefore be valuable to assess platelet responses mediated by diverse strains of bacteria. Here, I describe a method to study platelet integrin activation and granule release using flow cytometry, and a complementary method to study platelet aggregation using a dedicated platelet aggregometer. The combination of these methods represents a rapid and cost-effective strategy to provide mechanistic insight on the type of platelet response mediated by the bacteria.</p>}},
  author       = {{Shannon, Oonagh}},
  booktitle    = {{Methods in Molecular Biology}},
  issn         = {{10643745}},
  keywords     = {{Bacteria; Coagulation; Flow cytometry; Platelets; Streptococci}},
  language     = {{eng}},
  pages        = {{267--273}},
  publisher    = {{Humana Press}},
  series       = {{Methods in Molecular Biology}},
  title        = {{Determining platelet activation and aggregation in response to bacteria}},
  url          = {{http://dx.doi.org/10.1007/978-1-4939-6673-8_17}},
  doi          = {{10.1007/978-1-4939-6673-8_17}},
  volume       = {{1535}},
  year         = {{2017}},
}