Lignocellulose degradation for the bioeconomy: The potential of enzyme synergies between xylanases, ferulic acid esterase and laccase for the production of arabinoxylo-oligosaccharides
Schmitz, Eva LU ; Leontakianakou, Savvina LU ; Norlander, Siri LU ; Nordberg Karlsson, Eva LU
and Adlercreutz, Patrick
LU
(2022)
In Bioresource Technology
343.
- Abstract
- The success of establishing bioeconomies replacing current economies based on fossil resources largely depends on our ability to degrade recalcitrant lignocellulosic biomass. This study explores the potential of employing various enzymes acting synergistically on previously pretreated agricultural side streams (corn bran, oat hull, soluble and insoluble oat bran). Degrees of synergy (oligosaccharide yield obtained with the enzyme combination divided by the sum of yields obtained with individual enzymes) of up to 88 were obtained. Combinations of a ferulic acid esterase and xylanases resulted in synergy on all substrates, while a laccase and xylanases only acted synergistically on the more recalcitrant substrates. Synergy between different... (More)
- The success of establishing bioeconomies replacing current economies based on fossil resources largely depends on our ability to degrade recalcitrant lignocellulosic biomass. This study explores the potential of employing various enzymes acting synergistically on previously pretreated agricultural side streams (corn bran, oat hull, soluble and insoluble oat bran). Degrees of synergy (oligosaccharide yield obtained with the enzyme combination divided by the sum of yields obtained with individual enzymes) of up to 88 were obtained. Combinations of a ferulic acid esterase and xylanases resulted in synergy on all substrates, while a laccase and xylanases only acted synergistically on the more recalcitrant substrates. Synergy between different xylanases (glycoside hydrolase (GH) families 5 and 11) was observed particularly on oat hulls, producing a yield of 57%. The synergistic ability of the enzymes was found to be partly due to the increased enzyme stability when in combination with the substrates. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/d359945e-6e54-452f-ad19-048c1715c5fb
- author
- Schmitz, Eva
LU
; Leontakianakou, Savvina
LU
; Norlander, Siri
LU
; Nordberg Karlsson, Eva
LU
and Adlercreutz, Patrick
LU
- organization
- publishing date
- 2022
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Enzymatic synergy, Lignocellulose degradation, Recalcitrant biomass, Carbohydrate active enzymes, (arabino)xylooligosaccharides
- in
- Bioresource Technology
- volume
- 343
- article number
- 126114
- pages
- 10 pages
- publisher
- Elsevier
- external identifiers
-
- pmid:34648963
- scopus:85117127910
- ISSN
- 0960-8524
- DOI
- 10.1016/j.biortech.2021.126114
- language
- English
- LU publication?
- yes
- id
- d359945e-6e54-452f-ad19-048c1715c5fb
- date added to LUP
- 2021-10-18 10:20:59
- date last changed
- 2024-02-20 14:14:11
@article{d359945e-6e54-452f-ad19-048c1715c5fb,
abstract = {{The success of establishing bioeconomies replacing current economies based on fossil resources largely depends on our ability to degrade recalcitrant lignocellulosic biomass. This study explores the potential of employing various enzymes acting synergistically on previously pretreated agricultural side streams (corn bran, oat hull, soluble and insoluble oat bran). Degrees of synergy (oligosaccharide yield obtained with the enzyme combination divided by the sum of yields obtained with individual enzymes) of up to 88 were obtained. Combinations of a ferulic acid esterase and xylanases resulted in synergy on all substrates, while a laccase and xylanases only acted synergistically on the more recalcitrant substrates. Synergy between different xylanases (glycoside hydrolase (GH) families 5 and 11) was observed particularly on oat hulls, producing a yield of 57%. The synergistic ability of the enzymes was found to be partly due to the increased enzyme stability when in combination with the substrates.}},
author = {{Schmitz, Eva and Leontakianakou, Savvina and Norlander, Siri and Nordberg Karlsson, Eva and Adlercreutz, Patrick}},
issn = {{0960-8524}},
keywords = {{Enzymatic synergy; Lignocellulose degradation; Recalcitrant biomass; Carbohydrate active enzymes; (arabino)xylooligosaccharides}},
language = {{eng}},
publisher = {{Elsevier}},
series = {{Bioresource Technology}},
title = {{Lignocellulose degradation for the bioeconomy: The potential of enzyme synergies between xylanases, ferulic acid esterase and laccase for the production of arabinoxylo-oligosaccharides}},
url = {{http://dx.doi.org/10.1016/j.biortech.2021.126114}},
doi = {{10.1016/j.biortech.2021.126114}},
volume = {{343}},
year = {{2022}},
}