Sample preparation for single-cell whole chromosome analysis
Beech, Jason P. LU ; Adolfsson, Karl LU ; Holm, Stefan LU ; Yadegari, Farnaz ; Freitag, Camilla ; Fritzsche, Joachim ; Mir, Kalim U. and Tegenfeldt, Jonas O. LU
(2012)
16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
p.998-999
- Abstract
In this work we present an integrated system for whole chromosome analysis of single bacterium. Using whole genome barcoding techniques, which offer direct and rapid microscopic visualization of the entire genome in one field-of-view, we aim to rapidly identify individual bacterium. We are developing our device to achieve the crucial, and difficult process of isolating a bacterium, removing the DNA in one piece and transferring it to a nano-channel for visualisation. In order to achieve control over the bacteria we encapsulate them in agarose, using flow focusing. The encapsulated bacteria can then be transported in microchannels to proximity with the nanochannels and then chemically lysis can be performed. Following lysis the intact... (More)
In this work we present an integrated system for whole chromosome analysis of single bacterium. Using whole genome barcoding techniques, which offer direct and rapid microscopic visualization of the entire genome in one field-of-view, we aim to rapidly identify individual bacterium. We are developing our device to achieve the crucial, and difficult process of isolating a bacterium, removing the DNA in one piece and transferring it to a nano-channel for visualisation. In order to achieve control over the bacteria we encapsulate them in agarose, using flow focusing. The encapsulated bacteria can then be transported in microchannels to proximity with the nanochannels and then chemically lysis can be performed. Following lysis the intact genome can be extracted and transferred to the meandering nanochannel for analysis. We believe this device holds the potential to significantly decrease analysis times for single cell, whole genome analysis with the potential of opening up for automated, high-throughput genome analysis in microfluidic systems.
(Less)
- author
- Beech, Jason P.
LU
; Adolfsson, Karl
LU
; Holm, Stefan
LU
; Yadegari, Farnaz
; Freitag, Camilla
; Fritzsche, Joachim
; Mir, Kalim U.
and Tegenfeldt, Jonas O.
LU
- organization
- publishing date
- 2012-01-01
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- keywords
- Bacteria encapsulation, Chromosome analysis, Melting mapping, Nanochannels
- host publication
- Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
- pages
- 2 pages
- publisher
- Chemical and Biological Microsystems Society
- conference name
- 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
- conference location
- Okinawa, Japan
- conference dates
- 2012-10-28 - 2012-11-01
- external identifiers
-
- scopus:84901777389
- ISBN
- 9780979806452
- language
- English
- LU publication?
- yes
- id
- d4716564-e0a1-45f3-b8f9-13c927ba566c
- date added to LUP
- 2018-10-20 08:54:10
- date last changed
- 2023-08-07 14:00:30
@inproceedings{d4716564-e0a1-45f3-b8f9-13c927ba566c,
abstract = {{<p>In this work we present an integrated system for whole chromosome analysis of single bacterium. Using whole genome barcoding techniques, which offer direct and rapid microscopic visualization of the entire genome in one field-of-view, we aim to rapidly identify individual bacterium. We are developing our device to achieve the crucial, and difficult process of isolating a bacterium, removing the DNA in one piece and transferring it to a nano-channel for visualisation. In order to achieve control over the bacteria we encapsulate them in agarose, using flow focusing. The encapsulated bacteria can then be transported in microchannels to proximity with the nanochannels and then chemically lysis can be performed. Following lysis the intact genome can be extracted and transferred to the meandering nanochannel for analysis. We believe this device holds the potential to significantly decrease analysis times for single cell, whole genome analysis with the potential of opening up for automated, high-throughput genome analysis in microfluidic systems.</p>}},
author = {{Beech, Jason P. and Adolfsson, Karl and Holm, Stefan and Yadegari, Farnaz and Freitag, Camilla and Fritzsche, Joachim and Mir, Kalim U. and Tegenfeldt, Jonas O.}},
booktitle = {{Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012}},
isbn = {{9780979806452}},
keywords = {{Bacteria encapsulation; Chromosome analysis; Melting mapping; Nanochannels}},
language = {{eng}},
month = {{01}},
pages = {{998--999}},
publisher = {{Chemical and Biological Microsystems Society}},
title = {{Sample preparation for single-cell whole chromosome analysis}},
year = {{2012}},
}