A potentiometric enzyme electrode for monitoring in organic solvents
(1989) In Analytica Chimica Acta 219(C). p.27-36- Abstract
A potentiometric enzyme electrode is described for monitoring reactions in organic solvents. By use of an enzyme deposited on magnetic particles which are attracted to the tip of the electrode by means of a magnetic field, it is possible to produce an electrode in which the enzyme can easily be exchanged. As an example, studies of the chymotrypsin-catalyzed ester synthesis in diisopropyl ether and in toluene at varying water contents are reported. The results are consistent with those obtained from batch experiments. Operational behaviour and signal stability of the system makes this kind of potentiometric enzyme electrode attractive for monitoring bioorganic processes.
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https://lup.lub.lu.se/record/d78dcc02-4aa9-4fea-9da6-ebc733dfcec9
- author
- Miyabayashi, Akiyoshi ; Reslow, Mats ; Adlercreutz, Patrick LU and Mattiasson, Bo LU
- organization
- publishing date
- 1989-01-01
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Analytica Chimica Acta
- volume
- 219
- issue
- C
- pages
- 10 pages
- publisher
- Elsevier
- external identifiers
-
- scopus:0007001638
- ISSN
- 0003-2670
- DOI
- 10.1016/S0003-2670(00)80330-X
- language
- English
- LU publication?
- yes
- id
- d78dcc02-4aa9-4fea-9da6-ebc733dfcec9
- date added to LUP
- 2019-06-22 18:56:22
- date last changed
- 2021-01-03 09:57:05
@article{d78dcc02-4aa9-4fea-9da6-ebc733dfcec9, abstract = {{<p>A potentiometric enzyme electrode is described for monitoring reactions in organic solvents. By use of an enzyme deposited on magnetic particles which are attracted to the tip of the electrode by means of a magnetic field, it is possible to produce an electrode in which the enzyme can easily be exchanged. As an example, studies of the chymotrypsin-catalyzed ester synthesis in diisopropyl ether and in toluene at varying water contents are reported. The results are consistent with those obtained from batch experiments. Operational behaviour and signal stability of the system makes this kind of potentiometric enzyme electrode attractive for monitoring bioorganic processes.</p>}}, author = {{Miyabayashi, Akiyoshi and Reslow, Mats and Adlercreutz, Patrick and Mattiasson, Bo}}, issn = {{0003-2670}}, language = {{eng}}, month = {{01}}, number = {{C}}, pages = {{27--36}}, publisher = {{Elsevier}}, series = {{Analytica Chimica Acta}}, title = {{A potentiometric enzyme electrode for monitoring in organic solvents}}, url = {{http://dx.doi.org/10.1016/S0003-2670(00)80330-X}}, doi = {{10.1016/S0003-2670(00)80330-X}}, volume = {{219}}, year = {{1989}}, }