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Antibodies to a Mr-64000 islet cell protein in Swedish children with newly diagnosed Type 1 (insulin-dependent) diabetes

Christie, M. ; Landin-Olsson, M. LU ; Sundkvist, G. LU ; Dahlquist, G. ; Lernmark, Å LU orcid and Bækkeskov, S. (1988) In Diabetologia 31(8). p.597-602
Abstract

Sera from 40 Swedish children diagnosed as having Type 1 (insulin-dependent) diabetes mellitus during a one year period along with 40 age and geographically matched control subjects were tested for antibodies to a Mr-64000 islet protein by immunoprecipitation of 35S-methionine-labelled rat islet amphiphilic proteins. Of the 40 diabetic patients, 29 (73%) were found to be positive whereas all 40 control subjects were negative. Samples were also tested for titres of islet cell cytoplasmic antibodies by indirect immunofluorescence on frozen sections of human pancreas. In the diabetic group, 30 of the 40 patients (75%) were positive for islet cell cytoplasmic antibodies compared with 2 of the 40 control subjects (5%).... (More)

Sera from 40 Swedish children diagnosed as having Type 1 (insulin-dependent) diabetes mellitus during a one year period along with 40 age and geographically matched control subjects were tested for antibodies to a Mr-64000 islet protein by immunoprecipitation of 35S-methionine-labelled rat islet amphiphilic proteins. Of the 40 diabetic patients, 29 (73%) were found to be positive whereas all 40 control subjects were negative. Samples were also tested for titres of islet cell cytoplasmic antibodies by indirect immunofluorescence on frozen sections of human pancreas. In the diabetic group, 30 of the 40 patients (75%) were positive for islet cell cytoplasmic antibodies compared with 2 of the 40 control subjects (5%). A comparison of levels of antibodies to the Mr-64000 protein with islet cell cytoplasmic antibodies revealed a weak (rs=0.46), but significant (p<0.01) correlation between the two tests. There was no effect of age or sex on levels of antibodies to the Mr-64000 protein. These results in population-based diabetic children and control subjects demonstrate a high frequency of antibodies to the Mr-64000 protein at the time of clinical onset.

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author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
autoantibodies, autoantigens, autoimmune disease, islets of Langerhans, Type 1 (insulin-dependent) diabetes mellitus
in
Diabetologia
volume
31
issue
8
pages
6 pages
publisher
Springer
external identifiers
  • pmid:3065114
  • scopus:0023821719
ISSN
0012-186X
DOI
10.1007/BF00264766
language
English
LU publication?
yes
id
ec1b8323-fc48-4c39-b7d7-ed039854a58c
date added to LUP
2019-09-11 10:04:33
date last changed
2024-03-13 08:08:24
@article{ec1b8323-fc48-4c39-b7d7-ed039854a58c,
  abstract     = {{<p>Sera from 40 Swedish children diagnosed as having Type 1 (insulin-dependent) diabetes mellitus during a one year period along with 40 age and geographically matched control subjects were tested for antibodies to a M<sub>r</sub>-64000 islet protein by immunoprecipitation of <sup>35</sup>S-methionine-labelled rat islet amphiphilic proteins. Of the 40 diabetic patients, 29 (73%) were found to be positive whereas all 40 control subjects were negative. Samples were also tested for titres of islet cell cytoplasmic antibodies by indirect immunofluorescence on frozen sections of human pancreas. In the diabetic group, 30 of the 40 patients (75%) were positive for islet cell cytoplasmic antibodies compared with 2 of the 40 control subjects (5%). A comparison of levels of antibodies to the M<sub>r</sub>-64000 protein with islet cell cytoplasmic antibodies revealed a weak (r<sub>s</sub>=0.46), but significant (p&lt;0.01) correlation between the two tests. There was no effect of age or sex on levels of antibodies to the M<sub>r</sub>-64000 protein. These results in population-based diabetic children and control subjects demonstrate a high frequency of antibodies to the M<sub>r</sub>-64000 protein at the time of clinical onset.</p>}},
  author       = {{Christie, M. and Landin-Olsson, M. and Sundkvist, G. and Dahlquist, G. and Lernmark, Å and Bækkeskov, S.}},
  issn         = {{0012-186X}},
  keywords     = {{autoantibodies; autoantigens; autoimmune disease; islets of Langerhans; Type 1 (insulin-dependent) diabetes mellitus}},
  language     = {{eng}},
  month        = {{08}},
  number       = {{8}},
  pages        = {{597--602}},
  publisher    = {{Springer}},
  series       = {{Diabetologia}},
  title        = {{Antibodies to a M<sub>r</sub>-64000 islet cell protein in Swedish children with newly diagnosed Type 1 (insulin-dependent) diabetes}},
  url          = {{http://dx.doi.org/10.1007/BF00264766}},
  doi          = {{10.1007/BF00264766}},
  volume       = {{31}},
  year         = {{1988}},
}