Occupational second-hand smoke exposure : A comparative shotgun proteomics study on nasal epithelia from healthy restaurant workers
(2024) In Environmental Toxicology and Pharmacology 108.- Abstract
Non-smokers exposed to second-hand smoke (SHS) present risk of developing tobacco smoke-associated pathologies. To investigate the airway molecular response to SHS exposure that could be used in health risk assessment, comparative shotgun proteomics was performed on nasal epithelium from a group of healthy restaurant workers, non-smokers (never and former) exposed and not exposed to SHS in the workplace. HIF1α-glycolytic targets (GAPDH, TPI) and proteins related to xenobiotic metabolism, cell proliferation and differentiation leading to cancer (ADH1C, TUBB4B, EEF2) showed significant modulation in non-smokers exposed. In never smokers exposed, enrichment of glutathione metabolism pathway and EEF2-regulating protein synthesis in... (More)
Non-smokers exposed to second-hand smoke (SHS) present risk of developing tobacco smoke-associated pathologies. To investigate the airway molecular response to SHS exposure that could be used in health risk assessment, comparative shotgun proteomics was performed on nasal epithelium from a group of healthy restaurant workers, non-smokers (never and former) exposed and not exposed to SHS in the workplace. HIF1α-glycolytic targets (GAPDH, TPI) and proteins related to xenobiotic metabolism, cell proliferation and differentiation leading to cancer (ADH1C, TUBB4B, EEF2) showed significant modulation in non-smokers exposed. In never smokers exposed, enrichment of glutathione metabolism pathway and EEF2-regulating protein synthesis in genotoxic response were increased, while in former smokers exposed, proteins (LYZ, ATP1A1, SERPINB3) associated with tissue damage/regeneration, apoptosis inhibition and inflammation that may lead to asthma, COPD or cancer, were upregulated. The identified proteins are potential response and susceptibility/risk biomarkers for SHS exposure.
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- author
- Neves, Sofia ; Pacheco, Solange LU ; Vaz, Fátima ; James, Peter LU ; Simões, Tânia and Penque, Deborah
- organization
- publishing date
- 2024-06
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Cigarette smoke, Mass Spectrometry, Nasal epithelium, Protein network, Proteomics, Second-Hand Smoke
- in
- Environmental Toxicology and Pharmacology
- volume
- 108
- article number
- 104459
- publisher
- Elsevier
- external identifiers
-
- scopus:85192172558
- pmid:38685369
- ISSN
- 1382-6689
- DOI
- 10.1016/j.etap.2024.104459
- language
- English
- LU publication?
- yes
- id
- f9af0c3c-9ffe-44ac-a5a7-1aabfa26516f
- date added to LUP
- 2024-05-16 15:47:13
- date last changed
- 2024-07-11 21:01:03
@article{f9af0c3c-9ffe-44ac-a5a7-1aabfa26516f, abstract = {{<p>Non-smokers exposed to second-hand smoke (SHS) present risk of developing tobacco smoke-associated pathologies. To investigate the airway molecular response to SHS exposure that could be used in health risk assessment, comparative shotgun proteomics was performed on nasal epithelium from a group of healthy restaurant workers, non-smokers (never and former) exposed and not exposed to SHS in the workplace. HIF1α-glycolytic targets (GAPDH, TPI) and proteins related to xenobiotic metabolism, cell proliferation and differentiation leading to cancer (ADH1C, TUBB4B, EEF2) showed significant modulation in non-smokers exposed. In never smokers exposed, enrichment of glutathione metabolism pathway and EEF2-regulating protein synthesis in genotoxic response were increased, while in former smokers exposed, proteins (LYZ, ATP1A1, SERPINB3) associated with tissue damage/regeneration, apoptosis inhibition and inflammation that may lead to asthma, COPD or cancer, were upregulated. The identified proteins are potential response and susceptibility/risk biomarkers for SHS exposure.</p>}}, author = {{Neves, Sofia and Pacheco, Solange and Vaz, Fátima and James, Peter and Simões, Tânia and Penque, Deborah}}, issn = {{1382-6689}}, keywords = {{Cigarette smoke; Mass Spectrometry; Nasal epithelium; Protein network; Proteomics; Second-Hand Smoke}}, language = {{eng}}, publisher = {{Elsevier}}, series = {{Environmental Toxicology and Pharmacology}}, title = {{Occupational second-hand smoke exposure : A comparative shotgun proteomics study on nasal epithelia from healthy restaurant workers}}, url = {{http://dx.doi.org/10.1016/j.etap.2024.104459}}, doi = {{10.1016/j.etap.2024.104459}}, volume = {{108}}, year = {{2024}}, }