Advanced

Uricemia in juvenile pigs model : Effect of nephrectomy and potassium oxonate

Mosiichuk, N. LU ; Grujic, D. ; Woliński, J. LU ; Podpryatov, S. E. ; Podpriatov, S. S. ; Szczurek, P. LU ; Yatsenko, T. ; Shmihel, H. ; Drahanchuk, O. LU and Pierzynowski, S. G. LU , et al. (2019) In Journal of Animal and Feed Sciences 28(3). p.254-262
Abstract

Uric acid is the end product of dietary and endogenous purine metabolism in humans and higher primates. In all lower mammalian species it is converted to allantoin by liver uricase. The aim of this study was to investigate the uric acid turnover in pig model after nephrectomy surgery, fructose-enriched diet and potassium oxonate application. The first experiment was performed using 4 intact control pigs and 8 nephrectomized (5/6 nephrectomy) pigs. Both groups were fed high-fat diet enriched with 20% of fructose for 3 weeks. During the second experiment, as another approach to induce hyperuricemia, potassium oxonate solution (POx) was administered intravenously to 4 healthy pigs, once or twice per day. In the third preliminary experiment... (More)

Uric acid is the end product of dietary and endogenous purine metabolism in humans and higher primates. In all lower mammalian species it is converted to allantoin by liver uricase. The aim of this study was to investigate the uric acid turnover in pig model after nephrectomy surgery, fructose-enriched diet and potassium oxonate application. The first experiment was performed using 4 intact control pigs and 8 nephrectomized (5/6 nephrectomy) pigs. Both groups were fed high-fat diet enriched with 20% of fructose for 3 weeks. During the second experiment, as another approach to induce hyperuricemia, potassium oxonate solution (POx) was administered intravenously to 4 healthy pigs, once or twice per day. In the third preliminary experiment one healthy and two nephrectomized (9/10 nephrectomy) pigs were infused with POx to induce hyperuricemia. Results showed that 5/6 nephrecotomy did not affect plasma uric acid concentration for 25 days following surgery. The consumption of the high-fat diet enriched with 20% of fructose did not result in the rise of plasma uric acid, either in healthy or nephrectomized pigs. Administration of POx solution to healthy and 9/10 nephrectomized pigs resulted in significantly increased plasma uric acid concentrations for 18 h and 24 h, respectively, following a single POx infusion. The present study expands today available data on uric acid metabolism in pigs as a model for exploring uricemia in human with kidney dysfunction.

(Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; ; ; ; and , et al. (More)
; ; ; ; ; ; ; ; ; and (Less)
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Creatinine, Fructose, Hyperuricemia, Pigs, Potassium oxonate, Uric acid
in
Journal of Animal and Feed Sciences
volume
28
issue
3
pages
9 pages
publisher
KIELANOWSKI INST ANIMAL PHYSIOLOGY NUTRITION
external identifiers
  • scopus:85079270850
ISSN
1230-1388
DOI
10.22358/jafs/110436/2019
language
English
LU publication?
yes
id
0104c38b-1b33-4d44-b3ec-c1adc18a403a
date added to LUP
2020-02-26 13:47:51
date last changed
2020-02-27 01:57:28
@article{0104c38b-1b33-4d44-b3ec-c1adc18a403a,
  abstract     = {<p>Uric acid is the end product of dietary and endogenous purine metabolism in humans and higher primates. In all lower mammalian species it is converted to allantoin by liver uricase. The aim of this study was to investigate the uric acid turnover in pig model after nephrectomy surgery, fructose-enriched diet and potassium oxonate application. The first experiment was performed using 4 intact control pigs and 8 nephrectomized (5/6 nephrectomy) pigs. Both groups were fed high-fat diet enriched with 20% of fructose for 3 weeks. During the second experiment, as another approach to induce hyperuricemia, potassium oxonate solution (POx) was administered intravenously to 4 healthy pigs, once or twice per day. In the third preliminary experiment one healthy and two nephrectomized (9/10 nephrectomy) pigs were infused with POx to induce hyperuricemia. Results showed that 5/6 nephrecotomy did not affect plasma uric acid concentration for 25 days following surgery. The consumption of the high-fat diet enriched with 20% of fructose did not result in the rise of plasma uric acid, either in healthy or nephrectomized pigs. Administration of POx solution to healthy and 9/10 nephrectomized pigs resulted in significantly increased plasma uric acid concentrations for 18 h and 24 h, respectively, following a single POx infusion. The present study expands today available data on uric acid metabolism in pigs as a model for exploring uricemia in human with kidney dysfunction.</p>},
  author       = {Mosiichuk, N. and Grujic, D. and Woliński, J. and Podpryatov, S. E. and Podpriatov, S. S. and Szczurek, P. and Yatsenko, T. and Shmihel, H. and Drahanchuk, O. and Pierzynowski, S. G. and Goncharova Pierzynowska, K.},
  issn         = {1230-1388},
  language     = {eng},
  month        = {09},
  number       = {3},
  pages        = {254--262},
  publisher    = {KIELANOWSKI INST ANIMAL PHYSIOLOGY NUTRITION},
  series       = {Journal of Animal and Feed Sciences},
  title        = {Uricemia in juvenile pigs model : Effect of nephrectomy and potassium oxonate},
  url          = {http://dx.doi.org/10.22358/jafs/110436/2019},
  doi          = {10.22358/jafs/110436/2019},
  volume       = {28},
  year         = {2019},
}