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At low water activity alpha-chymotrypsin is more active in an ionic liquid than in non-ionic organic solvents

Eckstein, M ; Sesing, M ; Kragl, U and Adlercreutz, Patrick LU orcid (2002) In Biotechnology Letters 24(11). p.867-872
Abstract
The kinetics of the alpha-chymotrypsin catalysed transesterification of N-acetyl-l-phenylalanine ethyl ester with 1-butanol and the competing hydrolysis were evaluated at fixed water activity in two ionic liquids and two non-ionic organic solvents. In most respects the four solvents behaved similarly. However, at a water activity of 0.33, higher catalytic activity was observed in the ionic liquid, 1-butyl-3-methylimidazolium bis[(trifluoromethyl)sulfonyl]amide, than in the other solvents, and at a(w)=0.11 catalysis was only observed in this solvent.
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author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
water activity, alpha-chymotrypsin, ionic liquids
in
Biotechnology Letters
volume
24
issue
11
pages
867 - 872
publisher
Springer Science and Business Media B.V.
external identifiers
  • wos:000175752100003
  • scopus:0036273739
ISSN
1573-6776
DOI
10.1023/A:1015564608261
language
English
LU publication?
yes
id
014ecc11-1a14-4228-94f5-3ed36bb1d0c2 (old id 337279)
date added to LUP
2016-04-01 16:43:19
date last changed
2025-10-14 12:12:12
@article{014ecc11-1a14-4228-94f5-3ed36bb1d0c2,
  abstract     = {{The kinetics of the alpha-chymotrypsin catalysed transesterification of N-acetyl-l-phenylalanine ethyl ester with 1-butanol and the competing hydrolysis were evaluated at fixed water activity in two ionic liquids and two non-ionic organic solvents. In most respects the four solvents behaved similarly. However, at a water activity of 0.33, higher catalytic activity was observed in the ionic liquid, 1-butyl-3-methylimidazolium bis[(trifluoromethyl)sulfonyl]amide, than in the other solvents, and at a(w)=0.11 catalysis was only observed in this solvent.}},
  author       = {{Eckstein, M and Sesing, M and Kragl, U and Adlercreutz, Patrick}},
  issn         = {{1573-6776}},
  keywords     = {{water activity; alpha-chymotrypsin; ionic liquids}},
  language     = {{eng}},
  number       = {{11}},
  pages        = {{867--872}},
  publisher    = {{Springer Science and Business Media B.V.}},
  series       = {{Biotechnology Letters}},
  title        = {{At low water activity alpha-chymotrypsin is more active in an ionic liquid than in non-ionic organic solvents}},
  url          = {{http://dx.doi.org/10.1023/A:1015564608261}},
  doi          = {{10.1023/A:1015564608261}},
  volume       = {{24}},
  year         = {{2002}},
}