Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Biomonitoring of Dietary Heterocyclic Amines and Metabolites in Urine by Liquid Phase Microextraction: 2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP), a Possible Biomarker of Exposure to Dietary PhIP

Busquets, Rosa ; Frandsen, Henrik ; Jönsson, Jan Åke LU ; Puignou, Lluis ; Teresa Galceran, Maria and Skog, Kerstin LU (2013) In Chemical Research in Toxicology 26(2). p.233-240
Abstract
Heterocyclic amines (HCAs) are mutagenic/carcinogenic compounds formed at ng/g levels during frying meat or fish. The effect of the normal intake of dietary HCAs in humans and their involvement in the etiology of cancer are currently unknown. In this work, a new extraction method, liquid phase microextraction (LPME) with hollow fibers, and LC-MS/MS have been used for the first time to determine HCAs and metabolites in nonspiked human urine following a single meal of chicken cooked at 180 degrees C for 6 min. The total intake of HCAs was estimated to be 6 mu g, of which 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) accounted for about 1 mu g. The concentrations of PhIP in nonhydrolyzed urine samples ranged from 11.7 to 59.4 pg/g.... (More)
Heterocyclic amines (HCAs) are mutagenic/carcinogenic compounds formed at ng/g levels during frying meat or fish. The effect of the normal intake of dietary HCAs in humans and their involvement in the etiology of cancer are currently unknown. In this work, a new extraction method, liquid phase microextraction (LPME) with hollow fibers, and LC-MS/MS have been used for the first time to determine HCAs and metabolites in nonspiked human urine following a single meal of chicken cooked at 180 degrees C for 6 min. The total intake of HCAs was estimated to be 6 mu g, of which 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) accounted for about 1 mu g. The concentrations of PhIP in nonhydrolyzed urine samples ranged from 11.7 to 59.4 pg/g. The total amount of PhIP in urine ranged between 9.3 and 21.1 ng, which corresponds to 0.91-2.1% of the ingested PhIP. In addition, the urine levels of 4'-OH-PhIP (2-amino-1-methyl-6-(4'-hydroxy)phenylimidazo[4,5-b]pyridine) and 5-OH-PhIP (2-amino-1-methyl-6-(5-hydroxy)phenylimidazo[4,5-b]pyridine) also showed a narrow variation between the samples. The analysis of urine samples after acid hydrolysis did not give additional information but showed a notable increase in norharman in some cases. The obtained results suggest PhIP in urine as a possible biomarker of exposure to HCAs and the LPME and LC-MS/MS method as an appropriate strategy to biomonitor HCAs in urine. (Less)
Please use this url to cite or link to this publication:
author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Chemical Research in Toxicology
volume
26
issue
2
pages
233 - 240
publisher
The American Chemical Society (ACS)
external identifiers
  • wos:000315255400005
  • scopus:84874082126
  • pmid:23276304
ISSN
1520-5010
DOI
10.1021/tx3003966
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Applied Nutrition and Food Chemistry (011001300), Centre for Analysis and Synthesis (011001266)
id
0425892e-1f9a-4086-a117-35b209b6cde2 (old id 3674566)
date added to LUP
2016-04-01 10:45:37
date last changed
2023-08-31 10:54:22
@article{0425892e-1f9a-4086-a117-35b209b6cde2,
  abstract     = {{Heterocyclic amines (HCAs) are mutagenic/carcinogenic compounds formed at ng/g levels during frying meat or fish. The effect of the normal intake of dietary HCAs in humans and their involvement in the etiology of cancer are currently unknown. In this work, a new extraction method, liquid phase microextraction (LPME) with hollow fibers, and LC-MS/MS have been used for the first time to determine HCAs and metabolites in nonspiked human urine following a single meal of chicken cooked at 180 degrees C for 6 min. The total intake of HCAs was estimated to be 6 mu g, of which 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) accounted for about 1 mu g. The concentrations of PhIP in nonhydrolyzed urine samples ranged from 11.7 to 59.4 pg/g. The total amount of PhIP in urine ranged between 9.3 and 21.1 ng, which corresponds to 0.91-2.1% of the ingested PhIP. In addition, the urine levels of 4'-OH-PhIP (2-amino-1-methyl-6-(4'-hydroxy)phenylimidazo[4,5-b]pyridine) and 5-OH-PhIP (2-amino-1-methyl-6-(5-hydroxy)phenylimidazo[4,5-b]pyridine) also showed a narrow variation between the samples. The analysis of urine samples after acid hydrolysis did not give additional information but showed a notable increase in norharman in some cases. The obtained results suggest PhIP in urine as a possible biomarker of exposure to HCAs and the LPME and LC-MS/MS method as an appropriate strategy to biomonitor HCAs in urine.}},
  author       = {{Busquets, Rosa and Frandsen, Henrik and Jönsson, Jan Åke and Puignou, Lluis and Teresa Galceran, Maria and Skog, Kerstin}},
  issn         = {{1520-5010}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{233--240}},
  publisher    = {{The American Chemical Society (ACS)}},
  series       = {{Chemical Research in Toxicology}},
  title        = {{Biomonitoring of Dietary Heterocyclic Amines and Metabolites in Urine by Liquid Phase Microextraction: 2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP), a Possible Biomarker of Exposure to Dietary PhIP}},
  url          = {{http://dx.doi.org/10.1021/tx3003966}},
  doi          = {{10.1021/tx3003966}},
  volume       = {{26}},
  year         = {{2013}},
}