Biomonitoring of Dietary Heterocyclic Amines and Metabolites in Urine by Liquid Phase Microextraction: 2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP), a Possible Biomarker of Exposure to Dietary PhIP
(2013) In Chemical Research in Toxicology 26(2). p.233-240- Abstract
- Heterocyclic amines (HCAs) are mutagenic/carcinogenic compounds formed at ng/g levels during frying meat or fish. The effect of the normal intake of dietary HCAs in humans and their involvement in the etiology of cancer are currently unknown. In this work, a new extraction method, liquid phase microextraction (LPME) with hollow fibers, and LC-MS/MS have been used for the first time to determine HCAs and metabolites in nonspiked human urine following a single meal of chicken cooked at 180 degrees C for 6 min. The total intake of HCAs was estimated to be 6 mu g, of which 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) accounted for about 1 mu g. The concentrations of PhIP in nonhydrolyzed urine samples ranged from 11.7 to 59.4 pg/g.... (More)
- Heterocyclic amines (HCAs) are mutagenic/carcinogenic compounds formed at ng/g levels during frying meat or fish. The effect of the normal intake of dietary HCAs in humans and their involvement in the etiology of cancer are currently unknown. In this work, a new extraction method, liquid phase microextraction (LPME) with hollow fibers, and LC-MS/MS have been used for the first time to determine HCAs and metabolites in nonspiked human urine following a single meal of chicken cooked at 180 degrees C for 6 min. The total intake of HCAs was estimated to be 6 mu g, of which 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) accounted for about 1 mu g. The concentrations of PhIP in nonhydrolyzed urine samples ranged from 11.7 to 59.4 pg/g. The total amount of PhIP in urine ranged between 9.3 and 21.1 ng, which corresponds to 0.91-2.1% of the ingested PhIP. In addition, the urine levels of 4'-OH-PhIP (2-amino-1-methyl-6-(4'-hydroxy)phenylimidazo[4,5-b]pyridine) and 5-OH-PhIP (2-amino-1-methyl-6-(5-hydroxy)phenylimidazo[4,5-b]pyridine) also showed a narrow variation between the samples. The analysis of urine samples after acid hydrolysis did not give additional information but showed a notable increase in norharman in some cases. The obtained results suggest PhIP in urine as a possible biomarker of exposure to HCAs and the LPME and LC-MS/MS method as an appropriate strategy to biomonitor HCAs in urine. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3674566
- author
- Busquets, Rosa ; Frandsen, Henrik ; Jönsson, Jan Åke LU ; Puignou, Lluis ; Teresa Galceran, Maria and Skog, Kerstin LU
- organization
- publishing date
- 2013
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Chemical Research in Toxicology
- volume
- 26
- issue
- 2
- pages
- 233 - 240
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- wos:000315255400005
- scopus:84874082126
- pmid:23276304
- ISSN
- 1520-5010
- DOI
- 10.1021/tx3003966
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Applied Nutrition and Food Chemistry (011001300), Centre for Analysis and Synthesis (011001266)
- id
- 0425892e-1f9a-4086-a117-35b209b6cde2 (old id 3674566)
- date added to LUP
- 2016-04-01 10:45:37
- date last changed
- 2023-08-31 10:54:22
@article{0425892e-1f9a-4086-a117-35b209b6cde2, abstract = {{Heterocyclic amines (HCAs) are mutagenic/carcinogenic compounds formed at ng/g levels during frying meat or fish. The effect of the normal intake of dietary HCAs in humans and their involvement in the etiology of cancer are currently unknown. In this work, a new extraction method, liquid phase microextraction (LPME) with hollow fibers, and LC-MS/MS have been used for the first time to determine HCAs and metabolites in nonspiked human urine following a single meal of chicken cooked at 180 degrees C for 6 min. The total intake of HCAs was estimated to be 6 mu g, of which 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) accounted for about 1 mu g. The concentrations of PhIP in nonhydrolyzed urine samples ranged from 11.7 to 59.4 pg/g. The total amount of PhIP in urine ranged between 9.3 and 21.1 ng, which corresponds to 0.91-2.1% of the ingested PhIP. In addition, the urine levels of 4'-OH-PhIP (2-amino-1-methyl-6-(4'-hydroxy)phenylimidazo[4,5-b]pyridine) and 5-OH-PhIP (2-amino-1-methyl-6-(5-hydroxy)phenylimidazo[4,5-b]pyridine) also showed a narrow variation between the samples. The analysis of urine samples after acid hydrolysis did not give additional information but showed a notable increase in norharman in some cases. The obtained results suggest PhIP in urine as a possible biomarker of exposure to HCAs and the LPME and LC-MS/MS method as an appropriate strategy to biomonitor HCAs in urine.}}, author = {{Busquets, Rosa and Frandsen, Henrik and Jönsson, Jan Åke and Puignou, Lluis and Teresa Galceran, Maria and Skog, Kerstin}}, issn = {{1520-5010}}, language = {{eng}}, number = {{2}}, pages = {{233--240}}, publisher = {{The American Chemical Society (ACS)}}, series = {{Chemical Research in Toxicology}}, title = {{Biomonitoring of Dietary Heterocyclic Amines and Metabolites in Urine by Liquid Phase Microextraction: 2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP), a Possible Biomarker of Exposure to Dietary PhIP}}, url = {{http://dx.doi.org/10.1021/tx3003966}}, doi = {{10.1021/tx3003966}}, volume = {{26}}, year = {{2013}}, }