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Bacterial identification by optical mapping of genomic DNA in nanofluidic channels

Nyblom, My ; Müller, Vilhelm ; Johnning, Anna ; Wrande, Marie ; Dvirnas, Albertas LU ; Sriram, K. K. ; Giske, Christian G. ; Ambjörnsson, Tobias LU ; Sandegren, Linus and Kristiansson, Erik , et al. (2019) 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 In 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 p.821-822
Abstract

A variety of pathogenic bacteria can infect humans and the increase in bacteria resistant to common antibiotics is a large threat to human health worldwide. This work presents a method, based on optical DNA mapping (ODM) in nanofluidic channels, that can detect the type of bacterial present in a sample by matching the obtained maps of large DNA molecules to a database of fully assembled bacterial genomes. The extraction and labelling protocol has been designed to work for both Gram-positive and Gram-negative bacteria, not requiring any prior knowledge about the sample content.

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organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
Bacterial Identification, Nanofluidics, Optical DNA Mapping
host publication
23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
series title
23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
pages
2 pages
publisher
Chemical and Biological Microsystems Society
conference name
23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
conference location
Basel, Switzerland
conference dates
2019-10-27 - 2019-10-31
external identifiers
  • scopus:85094950178
ISBN
9781733419000
language
English
LU publication?
yes
id
04c69dca-ef51-4f0e-af63-cd74e8b868bd
date added to LUP
2020-11-24 08:21:21
date last changed
2024-03-20 21:34:51
@inproceedings{04c69dca-ef51-4f0e-af63-cd74e8b868bd,
  abstract     = {{<p>A variety of pathogenic bacteria can infect humans and the increase in bacteria resistant to common antibiotics is a large threat to human health worldwide. This work presents a method, based on optical DNA mapping (ODM) in nanofluidic channels, that can detect the type of bacterial present in a sample by matching the obtained maps of large DNA molecules to a database of fully assembled bacterial genomes. The extraction and labelling protocol has been designed to work for both Gram-positive and Gram-negative bacteria, not requiring any prior knowledge about the sample content.</p>}},
  author       = {{Nyblom, My and Müller, Vilhelm and Johnning, Anna and Wrande, Marie and Dvirnas, Albertas and Sriram, K. K. and Giske, Christian G. and Ambjörnsson, Tobias and Sandegren, Linus and Kristiansson, Erik and Westerlund, Fredrik}},
  booktitle    = {{23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019}},
  isbn         = {{9781733419000}},
  keywords     = {{Bacterial Identification; Nanofluidics; Optical DNA Mapping}},
  language     = {{eng}},
  pages        = {{821--822}},
  publisher    = {{Chemical and Biological Microsystems Society}},
  series       = {{23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019}},
  title        = {{Bacterial identification by optical mapping of genomic DNA in nanofluidic channels}},
  year         = {{2019}},
}