Bacterial identification by optical mapping of genomic DNA in nanofluidic channels
(2019) 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 In 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 p.821-822- Abstract
A variety of pathogenic bacteria can infect humans and the increase in bacteria resistant to common antibiotics is a large threat to human health worldwide. This work presents a method, based on optical DNA mapping (ODM) in nanofluidic channels, that can detect the type of bacterial present in a sample by matching the obtained maps of large DNA molecules to a database of fully assembled bacterial genomes. The extraction and labelling protocol has been designed to work for both Gram-positive and Gram-negative bacteria, not requiring any prior knowledge about the sample content.
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https://lup.lub.lu.se/record/04c69dca-ef51-4f0e-af63-cd74e8b868bd
- author
- organization
- publishing date
- 2019
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- keywords
- Bacterial Identification, Nanofluidics, Optical DNA Mapping
- host publication
- 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
- series title
- 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
- pages
- 2 pages
- publisher
- Chemical and Biological Microsystems Society
- conference name
- 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
- conference location
- Basel, Switzerland
- conference dates
- 2019-10-27 - 2019-10-31
- external identifiers
-
- scopus:85094950178
- ISBN
- 9781733419000
- language
- English
- LU publication?
- yes
- id
- 04c69dca-ef51-4f0e-af63-cd74e8b868bd
- date added to LUP
- 2020-11-24 08:21:21
- date last changed
- 2024-03-20 21:34:51
@inproceedings{04c69dca-ef51-4f0e-af63-cd74e8b868bd, abstract = {{<p>A variety of pathogenic bacteria can infect humans and the increase in bacteria resistant to common antibiotics is a large threat to human health worldwide. This work presents a method, based on optical DNA mapping (ODM) in nanofluidic channels, that can detect the type of bacterial present in a sample by matching the obtained maps of large DNA molecules to a database of fully assembled bacterial genomes. The extraction and labelling protocol has been designed to work for both Gram-positive and Gram-negative bacteria, not requiring any prior knowledge about the sample content.</p>}}, author = {{Nyblom, My and Müller, Vilhelm and Johnning, Anna and Wrande, Marie and Dvirnas, Albertas and Sriram, K. K. and Giske, Christian G. and Ambjörnsson, Tobias and Sandegren, Linus and Kristiansson, Erik and Westerlund, Fredrik}}, booktitle = {{23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019}}, isbn = {{9781733419000}}, keywords = {{Bacterial Identification; Nanofluidics; Optical DNA Mapping}}, language = {{eng}}, pages = {{821--822}}, publisher = {{Chemical and Biological Microsystems Society}}, series = {{23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019}}, title = {{Bacterial identification by optical mapping of genomic DNA in nanofluidic channels}}, year = {{2019}}, }