Multinodal Acoustic Trapping Enables High Capacity and High Throughput Enrichment of Extracellular Vesicles and Microparticles in miRNA and MS Proteomics Studies
(2021) In Analytical Chemistry 93(8). p.3929-3937- Abstract
We report a new design of an acoustophoretic trapping device with significantly increased capacity and throughput, compared to current commercial acoustic trapping systems. Acoustic trapping enables nanoparticle and extracellular vesicle (EV) enrichment without ultracentrifugation. Current commercial acoustic trapping technology uses an acoustic single-node resonance and typically operates at flow rates <50 μL/min, which limits the processing of the larger samples. Here, we use a larger capillary that supports an acoustic multinode resonance, which increased the seed particle capacity 40 times and throughput 25-40 times compared to single-node systems. The resulting increase in capacity and throughput was demonstrated by isolation of... (More)
We report a new design of an acoustophoretic trapping device with significantly increased capacity and throughput, compared to current commercial acoustic trapping systems. Acoustic trapping enables nanoparticle and extracellular vesicle (EV) enrichment without ultracentrifugation. Current commercial acoustic trapping technology uses an acoustic single-node resonance and typically operates at flow rates <50 μL/min, which limits the processing of the larger samples. Here, we use a larger capillary that supports an acoustic multinode resonance, which increased the seed particle capacity 40 times and throughput 25-40 times compared to single-node systems. The resulting increase in capacity and throughput was demonstrated by isolation of nanogram amounts of microRNA from acoustically trapped urinary EVs within 10 min. Additionally, the improved trapping performance enabled isolation of extracellular vesicles for downstream mass spectrometry analysis. This was demonstrated by the differential protein abundance profiling of urine samples (1-3 mL), derived from the non-trapped versus trapped urine samples.
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- author
- Broman, Axel LU ; Lenshof, Andreas LU ; Evander, Mikael LU ; Happonen, Lotta LU ; Ku, Anson LU ; Malmström, Johan LU and Laurell, Thomas LU
- organization
-
- NanoLund: Centre for Nanoscience
- MultiPark: Multidisciplinary research focused on Parkinson´s disease
- Department of Biomedical Engineering
- LUCC: Lund University Cancer Centre
- Acoustofluidics group (research group)
- BioMS (research group)
- Molecular Pathogenesis (research group)
- Infection Medicine (BMC)
- Medical Molecular Biology (research group)
- Division of Translational Cancer Research
- Mass Spectrometry
- epIgG (research group)
- SEBRA Sepsis and Bacterial Resistance Alliance (research group)
- Infection Medicine Proteomics (research group)
- publishing date
- 2021-03-02
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Analytical Chemistry
- volume
- 93
- issue
- 8
- pages
- 3929 - 3937
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- scopus:85101886685
- pmid:33592145
- pmid:33592145
- ISSN
- 1520-6882
- DOI
- 10.1021/acs.analchem.0c04772
- language
- English
- LU publication?
- yes
- id
- 07b2e218-0dfc-47a8-9abf-6a6d4651f56b
- date added to LUP
- 2021-03-10 17:08:28
- date last changed
- 2024-09-19 17:52:58
@article{07b2e218-0dfc-47a8-9abf-6a6d4651f56b, abstract = {{<p>We report a new design of an acoustophoretic trapping device with significantly increased capacity and throughput, compared to current commercial acoustic trapping systems. Acoustic trapping enables nanoparticle and extracellular vesicle (EV) enrichment without ultracentrifugation. Current commercial acoustic trapping technology uses an acoustic single-node resonance and typically operates at flow rates <50 μL/min, which limits the processing of the larger samples. Here, we use a larger capillary that supports an acoustic multinode resonance, which increased the seed particle capacity 40 times and throughput 25-40 times compared to single-node systems. The resulting increase in capacity and throughput was demonstrated by isolation of nanogram amounts of microRNA from acoustically trapped urinary EVs within 10 min. Additionally, the improved trapping performance enabled isolation of extracellular vesicles for downstream mass spectrometry analysis. This was demonstrated by the differential protein abundance profiling of urine samples (1-3 mL), derived from the non-trapped versus trapped urine samples. </p>}}, author = {{Broman, Axel and Lenshof, Andreas and Evander, Mikael and Happonen, Lotta and Ku, Anson and Malmström, Johan and Laurell, Thomas}}, issn = {{1520-6882}}, language = {{eng}}, month = {{03}}, number = {{8}}, pages = {{3929--3937}}, publisher = {{The American Chemical Society (ACS)}}, series = {{Analytical Chemistry}}, title = {{Multinodal Acoustic Trapping Enables High Capacity and High Throughput Enrichment of Extracellular Vesicles and Microparticles in miRNA and MS Proteomics Studies}}, url = {{http://dx.doi.org/10.1021/acs.analchem.0c04772}}, doi = {{10.1021/acs.analchem.0c04772}}, volume = {{93}}, year = {{2021}}, }