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Multinodal Acoustic Trapping Enables High Capacity and High Throughput Enrichment of Extracellular Vesicles and Microparticles in miRNA and MS Proteomics Studies

Broman, Axel LU ; Lenshof, Andreas LU ; Evander, Mikael LU ; Happonen, Lotta LU ; Ku, Anson LU ; Malmström, Johan LU and Laurell, Thomas LU (2021) In Analytical Chemistry 93(8). p.3929-3937
Abstract

We report a new design of an acoustophoretic trapping device with significantly increased capacity and throughput, compared to current commercial acoustic trapping systems. Acoustic trapping enables nanoparticle and extracellular vesicle (EV) enrichment without ultracentrifugation. Current commercial acoustic trapping technology uses an acoustic single-node resonance and typically operates at flow rates <50 μL/min, which limits the processing of the larger samples. Here, we use a larger capillary that supports an acoustic multinode resonance, which increased the seed particle capacity 40 times and throughput 25-40 times compared to single-node systems. The resulting increase in capacity and throughput was demonstrated by isolation of... (More)

We report a new design of an acoustophoretic trapping device with significantly increased capacity and throughput, compared to current commercial acoustic trapping systems. Acoustic trapping enables nanoparticle and extracellular vesicle (EV) enrichment without ultracentrifugation. Current commercial acoustic trapping technology uses an acoustic single-node resonance and typically operates at flow rates <50 μL/min, which limits the processing of the larger samples. Here, we use a larger capillary that supports an acoustic multinode resonance, which increased the seed particle capacity 40 times and throughput 25-40 times compared to single-node systems. The resulting increase in capacity and throughput was demonstrated by isolation of nanogram amounts of microRNA from acoustically trapped urinary EVs within 10 min. Additionally, the improved trapping performance enabled isolation of extracellular vesicles for downstream mass spectrometry analysis. This was demonstrated by the differential protein abundance profiling of urine samples (1-3 mL), derived from the non-trapped versus trapped urine samples.

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author
; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Analytical Chemistry
volume
93
issue
8
pages
3929 - 3937
publisher
The American Chemical Society (ACS)
external identifiers
  • pmid:33592145
  • scopus:85101886685
  • pmid:33592145
ISSN
1520-6882
DOI
10.1021/acs.analchem.0c04772
language
English
LU publication?
yes
id
07b2e218-0dfc-47a8-9abf-6a6d4651f56b
date added to LUP
2021-03-10 17:08:28
date last changed
2021-06-10 03:00:09
@article{07b2e218-0dfc-47a8-9abf-6a6d4651f56b,
  abstract     = {<p>We report a new design of an acoustophoretic trapping device with significantly increased capacity and throughput, compared to current commercial acoustic trapping systems. Acoustic trapping enables nanoparticle and extracellular vesicle (EV) enrichment without ultracentrifugation. Current commercial acoustic trapping technology uses an acoustic single-node resonance and typically operates at flow rates &lt;50 μL/min, which limits the processing of the larger samples. Here, we use a larger capillary that supports an acoustic multinode resonance, which increased the seed particle capacity 40 times and throughput 25-40 times compared to single-node systems. The resulting increase in capacity and throughput was demonstrated by isolation of nanogram amounts of microRNA from acoustically trapped urinary EVs within 10 min. Additionally, the improved trapping performance enabled isolation of extracellular vesicles for downstream mass spectrometry analysis. This was demonstrated by the differential protein abundance profiling of urine samples (1-3 mL), derived from the non-trapped versus trapped urine samples. </p>},
  author       = {Broman, Axel and Lenshof, Andreas and Evander, Mikael and Happonen, Lotta and Ku, Anson and Malmström, Johan and Laurell, Thomas},
  issn         = {1520-6882},
  language     = {eng},
  month        = {03},
  number       = {8},
  pages        = {3929--3937},
  publisher    = {The American Chemical Society (ACS)},
  series       = {Analytical Chemistry},
  title        = {Multinodal Acoustic Trapping Enables High Capacity and High Throughput Enrichment of Extracellular Vesicles and Microparticles in miRNA and MS Proteomics Studies},
  url          = {http://dx.doi.org/10.1021/acs.analchem.0c04772},
  doi          = {10.1021/acs.analchem.0c04772},
  volume       = {93},
  year         = {2021},
}