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Oxidative Depolymerization of Lignosulfonates for Muconic Acid Production Using Recombinant Pseudomonas putida CJ475

Bekirovska, Selda LU orcid ; Lund, Fredrik LU ; Ajakaiye Jensen, Lucy I. LU ; Hulteberg, Christian P. LU orcid ; Gorwa-Grauslund, Marie F. LU and Abdelaziz, Omar Y. (2025) In Waste and Biomass Valorization
Abstract
Lignosulfonate, a macromolecular byproduct derived from the paper and
pulp industry, is a promising feedstock for bioconversion processes due
to its rich carbon content and widespread availability. We here present
an approach for the production of muconic acid through the optimization
of the oxidative depolymerization of lignosulfonate and its further
conversion using a recombinant Pseudomonas putida strain. This
process yielded a stream containing a diverse mixture of
low-molecular-weight aromatic compounds, the highest yield being that of
vanillin, of 5.77 wt%. When bioconversion was performed with the P. putida
strain CJ475, previously engineered for the conversion of vanillin... (More)
Lignosulfonate, a macromolecular byproduct derived from the paper and
pulp industry, is a promising feedstock for bioconversion processes due
to its rich carbon content and widespread availability. We here present
an approach for the production of muconic acid through the optimization
of the oxidative depolymerization of lignosulfonate and its further
conversion using a recombinant Pseudomonas putida strain. This
process yielded a stream containing a diverse mixture of
low-molecular-weight aromatic compounds, the highest yield being that of
vanillin, of 5.77 wt%. When bioconversion was performed with the P. putida
strain CJ475, previously engineered for the conversion of vanillin to
muconic acid, no conversion was initially observed due to the inhibitory
effect of the depolymerized lignosulfonate fraction on bacterial
growth. However, a simple additional extraction step with ethyl acetate
led to the successful conversion of the substrate with a yield of
1.3 ± 0.06 mol muconic acid/mol vanillin in the stream. This study
demonstrates the feasibility of utilizing oxidatively depolymerized
lignosulfonates as a sustainable carbon source for biobased muconic acid
production. It also marks a significant step forward in the utilization
of lignocellulosic biomass for sustainable high-value biochemical
production, as well as providing valuable insights into the development
of bioprocesses utilizing complex, lignin-rich feedstocks. (Less)
Please use this url to cite or link to this publication:
author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
epub
subject
keywords
Biobased chemicals, Biorefinery systems, Lignin depolymerization, Lignin utilization, Microbial upgrading, Sustainable resources
in
Waste and Biomass Valorization
publisher
Springer Science and Business Media B.V.
external identifiers
  • scopus:85217206940
ISSN
1877-2641
DOI
10.1007/s12649-025-02890-4
language
English
LU publication?
yes
additional info
Publisher Copyright: © The Author(s) 2025.
id
080d20ee-3130-414c-b4af-d52bb2ac353e
date added to LUP
2025-02-26 14:39:40
date last changed
2025-04-04 14:06:41
@article{080d20ee-3130-414c-b4af-d52bb2ac353e,
  abstract     = {{Lignosulfonate, a macromolecular byproduct derived from the paper and <br>
pulp industry, is a promising feedstock for bioconversion processes due <br>
to its rich carbon content and widespread availability. We here present <br>
an approach for the production of muconic acid through the optimization <br>
of the oxidative depolymerization of lignosulfonate and its further <br>
conversion using a recombinant <i>Pseudomonas putida</i> strain. This <br>
process yielded a stream containing a diverse mixture of <br>
low-molecular-weight aromatic compounds, the highest yield being that of<br>
 vanillin, of 5.77 wt%. When bioconversion was performed with the <i>P. putida</i><br>
 strain CJ475, previously engineered for the conversion of vanillin to <br>
muconic acid, no conversion was initially observed due to the inhibitory<br>
 effect of the depolymerized lignosulfonate fraction on bacterial <br>
growth. However, a simple additional extraction step with ethyl acetate <br>
led to the successful conversion of the substrate with a yield of <br>
1.3 ± 0.06 mol muconic acid/mol vanillin in the stream. This study <br>
demonstrates the feasibility of utilizing oxidatively depolymerized <br>
lignosulfonates as a sustainable carbon source for biobased muconic acid<br>
 production. It also marks a significant step forward in the utilization<br>
 of lignocellulosic biomass for sustainable high-value biochemical <br>
production, as well as providing valuable insights into the development <br>
of bioprocesses utilizing complex, lignin-rich feedstocks.}},
  author       = {{Bekirovska, Selda and Lund, Fredrik and Ajakaiye Jensen, Lucy I. and Hulteberg, Christian P. and Gorwa-Grauslund, Marie F. and Abdelaziz, Omar Y.}},
  issn         = {{1877-2641}},
  keywords     = {{Biobased chemicals; Biorefinery systems; Lignin depolymerization; Lignin utilization; Microbial upgrading; Sustainable resources}},
  language     = {{eng}},
  publisher    = {{Springer Science and Business Media B.V.}},
  series       = {{Waste and Biomass Valorization}},
  title        = {{Oxidative Depolymerization of Lignosulfonates for Muconic Acid Production Using Recombinant <i>Pseudomonas putida</i> CJ475}},
  url          = {{http://dx.doi.org/10.1007/s12649-025-02890-4}},
  doi          = {{10.1007/s12649-025-02890-4}},
  year         = {{2025}},
}