Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Controlled deposition of human neural stem cells on fiber substrates using gel encapsulation

Hörberg, Carl Johan LU orcid ; Arvidsson, Martin ; Sandberg, Axel ; O’Carroll, David LU ; Johansson, Fredrik LU and Johansson, Ulrica Englund LU (2026) In PLOS ONE 21(2).
Abstract

Electrospinning is a versatile technique for manufacturing micro-nano diameter fibers and has been used extensively for tissue engineering in vivo and advanced cell culture in vitro. Standard means of seeding cells onto such substrates typically offer no control over cell distribution, yielding dispersed, heterogeneous and low concentrations of cells. In this article, we investigate the viability of using a simple bioprinting-inspired device for seeding gel-encapsulated cells onto fiber substrates. Using human neural stem cells, we were able to consistently seed cells with spatial control. We examined their long-term development, showing viable cells and normal differentiation potential. Furthermore, this device was able to seed on... (More)

Electrospinning is a versatile technique for manufacturing micro-nano diameter fibers and has been used extensively for tissue engineering in vivo and advanced cell culture in vitro. Standard means of seeding cells onto such substrates typically offer no control over cell distribution, yielding dispersed, heterogeneous and low concentrations of cells. In this article, we investigate the viability of using a simple bioprinting-inspired device for seeding gel-encapsulated cells onto fiber substrates. Using human neural stem cells, we were able to consistently seed cells with spatial control. We examined their long-term development, showing viable cells and normal differentiation potential. Furthermore, this device was able to seed on multiple sites within a single substrate, creating isolated populations and demonstrating the potential for this approach as a low-cost alternative to bioprinting systems, which is also applicable to somewhat challenging 3D substrates like electrospun fibers.

(Less)
Please use this url to cite or link to this publication:
author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
PLOS ONE
volume
21
issue
2
article number
e0341624
publisher
Public Library of Science (PLoS)
external identifiers
  • scopus:105031154607
  • pmid:41739838
ISSN
1932-6203
DOI
10.1371/journal.pone.0341624
language
English
LU publication?
yes
additional info
Publisher Copyright: © 2026 Hörberg et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
id
082d7096-e1d7-45d2-83ad-2a9e3206b871
date added to LUP
2026-04-07 15:04:01
date last changed
2026-04-10 13:55:25
@article{082d7096-e1d7-45d2-83ad-2a9e3206b871,
  abstract     = {{<p>Electrospinning is a versatile technique for manufacturing micro-nano diameter fibers and has been used extensively for tissue engineering in vivo and advanced cell culture in vitro. Standard means of seeding cells onto such substrates typically offer no control over cell distribution, yielding dispersed, heterogeneous and low concentrations of cells. In this article, we investigate the viability of using a simple bioprinting-inspired device for seeding gel-encapsulated cells onto fiber substrates. Using human neural stem cells, we were able to consistently seed cells with spatial control. We examined their long-term development, showing viable cells and normal differentiation potential. Furthermore, this device was able to seed on multiple sites within a single substrate, creating isolated populations and demonstrating the potential for this approach as a low-cost alternative to bioprinting systems, which is also applicable to somewhat challenging 3D substrates like electrospun fibers.</p>}},
  author       = {{Hörberg, Carl Johan and Arvidsson, Martin and Sandberg, Axel and O’Carroll, David and Johansson, Fredrik and Johansson, Ulrica Englund}},
  issn         = {{1932-6203}},
  language     = {{eng}},
  number       = {{2}},
  publisher    = {{Public Library of Science (PLoS)}},
  series       = {{PLOS ONE}},
  title        = {{Controlled deposition of human neural stem cells on fiber substrates using gel encapsulation}},
  url          = {{http://dx.doi.org/10.1371/journal.pone.0341624}},
  doi          = {{10.1371/journal.pone.0341624}},
  volume       = {{21}},
  year         = {{2026}},
}