Pax6 promotes neurogenesis in human neural stem cells.
Kallur, Therese LU ; Gisler, Ramiro LU ; Lindvall, Olle LU and Kokaia, Zaal LU
(2008)
In Molecular and Cellular Neuroscience
38(4).
p.616-628
- Abstract
- During brain embryogenesis, transcription factors drive stem cells towards neuronal fate. Here we show that the transcription factor Pax6 increased in vitro generation of neurons from striatal but not cortical neural stem cells (NSCs), derived from 6 to 9 weeks old human fetuses, without affecting survival and proliferation. Overexpression of mouse Pax6 produced increased numbers of GABA+ and DARPP-32+ (characteristic of striatum) but not glutamate+ neurons (characteristic of cortex). Pax6-overexpressing cells survived and migrated to the same extent as control cells at 1 month after intrastriatal transplantation into newborn rats and generated more neuroblasts. Overexpression of mouse Pax6 in human NSCs also leads to altered levels of... (More)
- During brain embryogenesis, transcription factors drive stem cells towards neuronal fate. Here we show that the transcription factor Pax6 increased in vitro generation of neurons from striatal but not cortical neural stem cells (NSCs), derived from 6 to 9 weeks old human fetuses, without affecting survival and proliferation. Overexpression of mouse Pax6 produced increased numbers of GABA+ and DARPP-32+ (characteristic of striatum) but not glutamate+ neurons (characteristic of cortex). Pax6-overexpressing cells survived and migrated to the same extent as control cells at 1 month after intrastriatal transplantation into newborn rats and generated more neuroblasts. Overexpression of mouse Pax6 in human NSCs also leads to altered levels of lineage-appropriate genes as revealed by Q-PCR. Our data suggest that Pax6 function is conserved between species since its overexpression activates similar genes in mouse and human NSCs. Also, that Pax6 overexpression in striatal NSCs increases the number of neurons but their region-specificity is maintained. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1181648
- author
- Kallur, Therese
LU
; Gisler, Ramiro
LU
; Lindvall, Olle
LU
and Kokaia, Zaal
LU
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Molecular and Cellular Neuroscience
- volume
- 38
- issue
- 4
- pages
- 616 - 628
- publisher
- Elsevier
- external identifiers
-
- wos:000258347300017
- pmid:18595732
- scopus:47749138860
- pmid:18595732
- ISSN
- 1044-7431
- DOI
- 10.1016/j.mcn.2008.05.010
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Restorative Neurology (0131000160), Faculty of Medicine (000022000), Neurology, Lund (013027000)
- id
- 0a46789a-af5a-46d7-aacb-e1fca95cafa8 (old id 1181648)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/18595732?dopt=Abstract
- date added to LUP
- 2016-04-04 08:30:35
- date last changed
- 2022-01-29 03:32:11
@article{0a46789a-af5a-46d7-aacb-e1fca95cafa8,
abstract = {{During brain embryogenesis, transcription factors drive stem cells towards neuronal fate. Here we show that the transcription factor Pax6 increased in vitro generation of neurons from striatal but not cortical neural stem cells (NSCs), derived from 6 to 9 weeks old human fetuses, without affecting survival and proliferation. Overexpression of mouse Pax6 produced increased numbers of GABA+ and DARPP-32+ (characteristic of striatum) but not glutamate+ neurons (characteristic of cortex). Pax6-overexpressing cells survived and migrated to the same extent as control cells at 1 month after intrastriatal transplantation into newborn rats and generated more neuroblasts. Overexpression of mouse Pax6 in human NSCs also leads to altered levels of lineage-appropriate genes as revealed by Q-PCR. Our data suggest that Pax6 function is conserved between species since its overexpression activates similar genes in mouse and human NSCs. Also, that Pax6 overexpression in striatal NSCs increases the number of neurons but their region-specificity is maintained.}},
author = {{Kallur, Therese and Gisler, Ramiro and Lindvall, Olle and Kokaia, Zaal}},
issn = {{1044-7431}},
language = {{eng}},
number = {{4}},
pages = {{616--628}},
publisher = {{Elsevier}},
series = {{Molecular and Cellular Neuroscience}},
title = {{Pax6 promotes neurogenesis in human neural stem cells.}},
url = {{http://dx.doi.org/10.1016/j.mcn.2008.05.010}},
doi = {{10.1016/j.mcn.2008.05.010}},
volume = {{38}},
year = {{2008}},
}