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Quantitative proteomic analysis of human peripheral nerves from subjects with type 2 diabetes

Ising, Erik LU ; Åhrman, Emma LU ; Thomsen, Niels O B LU ; Eriksson, Karl-Fredrik LU ; Malmström, Johan LU orcid and Dahlin, Lars B LU orcid (2021) In Diabetic Medicine: A journal of the British Diabetic Association 38(11).
Abstract

AIMS: Diabetic peripheral neuropathy (DPN) is a common and severe complication to type 2 diabetes (T2D). The pathogenesis of DPN is not fully known, but several pathways and gene polymorphisms contributing to DPN are described. DPN can be studied using nerve biopsies, but studies on the proteome of the nerve itself, and its surrounding tissue as a whole, are lacking. Studies on the posterior interosseous nerve (PIN) have proposed PIN a useful indicator of DPN.

METHODS: A quantitative mass spectrometry-based proteomics analysis was made of peripheral nerves from age- and gender-matched living human male tissue donors; nine T2D subjects, with decreased sural nerve action potentials indicating DPN, and six controls without T2D, with... (More)

AIMS: Diabetic peripheral neuropathy (DPN) is a common and severe complication to type 2 diabetes (T2D). The pathogenesis of DPN is not fully known, but several pathways and gene polymorphisms contributing to DPN are described. DPN can be studied using nerve biopsies, but studies on the proteome of the nerve itself, and its surrounding tissue as a whole, are lacking. Studies on the posterior interosseous nerve (PIN) have proposed PIN a useful indicator of DPN.

METHODS: A quantitative mass spectrometry-based proteomics analysis was made of peripheral nerves from age- and gender-matched living human male tissue donors; nine T2D subjects, with decreased sural nerve action potentials indicating DPN, and six controls without T2D, with normal electrophysiology results.

RESULTS: A total of 2617 proteins were identified. Linear regression was used to discover which proteins were differentially expressed between T2D and controls. Only soft signals were found. Therefore, clustering of the 500 most variable proteins were made in order to find clusters of similar proteins in T2D subjects and healthy controls.

CONCLUSIONS: This feasibility study shows, for the first time, that the use of quantitative mass spectrometry enables quantification of proteins from nerve biopsies from subjects with and without T2D, which may aid in finding biomarkers of importance to DPN development.

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author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
diabetes mellitus, diabetic neuropathies, peripheral neuropathies, proteome, type 2
in
Diabetic Medicine: A journal of the British Diabetic Association
volume
38
issue
11
publisher
Wiley-Blackwell
external identifiers
  • pmid:34309080
  • scopus:85111781949
ISSN
1464-5491
DOI
10.1111/dme.14658
language
English
LU publication?
yes
id
0e179a61-e14b-41f3-8960-74e1e36aab46
date added to LUP
2021-08-04 15:45:12
date last changed
2024-02-20 09:02:50
@article{0e179a61-e14b-41f3-8960-74e1e36aab46,
  abstract     = {{<p>AIMS: Diabetic peripheral neuropathy (DPN) is a common and severe complication to type 2 diabetes (T2D). The pathogenesis of DPN is not fully known, but several pathways and gene polymorphisms contributing to DPN are described. DPN can be studied using nerve biopsies, but studies on the proteome of the nerve itself, and its surrounding tissue as a whole, are lacking. Studies on the posterior interosseous nerve (PIN) have proposed PIN a useful indicator of DPN.</p><p>METHODS: A quantitative mass spectrometry-based proteomics analysis was made of peripheral nerves from age- and gender-matched living human male tissue donors; nine T2D subjects, with decreased sural nerve action potentials indicating DPN, and six controls without T2D, with normal electrophysiology results.</p><p>RESULTS: A total of 2617 proteins were identified. Linear regression was used to discover which proteins were differentially expressed between T2D and controls. Only soft signals were found. Therefore, clustering of the 500 most variable proteins were made in order to find clusters of similar proteins in T2D subjects and healthy controls.</p><p>CONCLUSIONS: This feasibility study shows, for the first time, that the use of quantitative mass spectrometry enables quantification of proteins from nerve biopsies from subjects with and without T2D, which may aid in finding biomarkers of importance to DPN development.</p>}},
  author       = {{Ising, Erik and Åhrman, Emma and Thomsen, Niels O B and Eriksson, Karl-Fredrik and Malmström, Johan and Dahlin, Lars B}},
  issn         = {{1464-5491}},
  keywords     = {{diabetes mellitus; diabetic neuropathies; peripheral neuropathies; proteome; type 2}},
  language     = {{eng}},
  month        = {{07}},
  number       = {{11}},
  publisher    = {{Wiley-Blackwell}},
  series       = {{Diabetic Medicine: A journal of the British Diabetic Association}},
  title        = {{Quantitative proteomic analysis of human peripheral nerves from subjects with type 2 diabetes}},
  url          = {{http://dx.doi.org/10.1111/dme.14658}},
  doi          = {{10.1111/dme.14658}},
  volume       = {{38}},
  year         = {{2021}},
}