Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Aspects of infant food factors on intestinal inflammation and effects of probiotics in celiac disease autoimmunity

Oscarsson, Elin LU (2021) In Doctoral dissertation series 2021:1
Abstract
Aims: The overall aim of this thesis was to study dietary components with focus on exposure and ability to affect gut
permeability, peripheral immune response and gut microbiota in children at genetic risk of developing CD. The
specific aims were to study presence of food components that might induce intestinal inflammation, evaluate
possible effects on intestinal permeability by food emulsifiers, investigate if two different strains of Lactobacillaceae
affect ongoing CD autoimmunity and gut microbiota composition.
Methods: Porridges and milk cereal drinks were analysed for presence of endotoxins. The quantity of bacteria was
determined using culturing methods and identified with Sanger sequencing. Enterotoxin... (More)
Aims: The overall aim of this thesis was to study dietary components with focus on exposure and ability to affect gut
permeability, peripheral immune response and gut microbiota in children at genetic risk of developing CD. The
specific aims were to study presence of food components that might induce intestinal inflammation, evaluate
possible effects on intestinal permeability by food emulsifiers, investigate if two different strains of Lactobacillaceae
affect ongoing CD autoimmunity and gut microbiota composition.
Methods: Porridges and milk cereal drinks were analysed for presence of endotoxins. The quantity of bacteria was
determined using culturing methods and identified with Sanger sequencing. Enterotoxin producing genes were
analysed through multiplex PCR. Modification of intestinal permeability ex vivo in rat by emulsifiers were determined
with Ussing diffusion chamber. Changes in immune response after intake of probiotic bacteria or placebo in 78
children with CDA were measured using flow cytometry while changes in tissue transglutaminase autoantibody
levels were measured in radioligand binding assays. Effects of probiotic intake on the gut microbiota were
determined by 16SrRNA sequencing.
Results: Endotoxin concentrations ranged from 1400 to 24200 EU/g powder and bacteria were present in all
products. Polysorbate 80 (P80) was able to increase the permeability in the rat intestine ex vivo to a greater extent
compared with carboxymethyl cellulose and beta-lactoglobulin. The probiotic bacteria seemed to inhibit the
peripheral immune response in children with CDA compared with placebo and affected the gut microbiota towards
a composition more similar to healthy controls.
Conclusion: Endotoxins are present in powdered infant cereal-based foods and the amount of living bacteria might
be substantial. P80 appeared to increase the intestinal permeability ex vivo in rat more compared with the other
emulsifiers. Probiotic supplementation decreased the level of NKT-cells in children with CDA and influenced the
abundance of specific bacterial amplicon sequence variants in the gut microbiota. (Less)
Please use this url to cite or link to this publication:
author
supervisor
opponent
  • Professor Öhman, Lena, Sahlgrenska akademin, Göteborg
organization
publishing date
type
Thesis
publication status
published
subject
keywords
Endotoxin, food emulsifiers, intestinal permeability, probiotics, celiac disease, celiac disease autoimmunity
in
Doctoral dissertation series 2021:1
pages
73 pages
publisher
Lund University, Faculty of Medicine
defense location
Lilla aulan, Jan Waldenströms gata 5, Skånes Universitetssjukhus i Malmö
defense date
2021-01-29 09:15:00
ISSN
1652-8220
ISBN
978-91-8021-013-3
language
English
LU publication?
yes
id
0f5c3be6-9b97-44f0-bc1a-29713a6e2cec
date added to LUP
2021-01-08 16:27:06
date last changed
2021-01-12 15:50:27
@phdthesis{0f5c3be6-9b97-44f0-bc1a-29713a6e2cec,
  abstract     = {{Aims: The overall aim of this thesis was to study dietary components with focus on exposure and ability to affect gut<br/>permeability, peripheral immune response and gut microbiota in children at genetic risk of developing CD. The<br/>specific aims were to study presence of food components that might induce intestinal inflammation, evaluate<br/>possible effects on intestinal permeability by food emulsifiers, investigate if two different strains of Lactobacillaceae<br/>affect ongoing CD autoimmunity and gut microbiota composition.<br/>Methods: Porridges and milk cereal drinks were analysed for presence of endotoxins. The quantity of bacteria was<br/>determined using culturing methods and identified with Sanger sequencing. Enterotoxin producing genes were<br/>analysed through multiplex PCR. Modification of intestinal permeability ex vivo in rat by emulsifiers were determined<br/>with Ussing diffusion chamber. Changes in immune response after intake of probiotic bacteria or placebo in 78<br/>children with CDA were measured using flow cytometry while changes in tissue transglutaminase autoantibody<br/>levels were measured in radioligand binding assays. Effects of probiotic intake on the gut microbiota were<br/>determined by 16SrRNA sequencing.<br/>Results: Endotoxin concentrations ranged from 1400 to 24200 EU/g powder and bacteria were present in all<br/>products. Polysorbate 80 (P80) was able to increase the permeability in the rat intestine ex vivo to a greater extent<br/>compared with carboxymethyl cellulose and beta-lactoglobulin. The probiotic bacteria seemed to inhibit the<br/>peripheral immune response in children with CDA compared with placebo and affected the gut microbiota towards<br/>a composition more similar to healthy controls.<br/>Conclusion: Endotoxins are present in powdered infant cereal-based foods and the amount of living bacteria might<br/>be substantial. P80 appeared to increase the intestinal permeability ex vivo in rat more compared with the other<br/>emulsifiers. Probiotic supplementation decreased the level of NKT-cells in children with CDA and influenced the<br/>abundance of specific bacterial amplicon sequence variants in the gut microbiota.}},
  author       = {{Oscarsson, Elin}},
  isbn         = {{978-91-8021-013-3}},
  issn         = {{1652-8220}},
  keywords     = {{Endotoxin, food emulsifiers, intestinal permeability, probiotics, celiac disease, celiac disease autoimmunity}},
  language     = {{eng}},
  publisher    = {{Lund University, Faculty of Medicine}},
  school       = {{Lund University}},
  series       = {{Doctoral dissertation series 2021:1}},
  title        = {{Aspects of infant food factors on intestinal inflammation and effects of probiotics in celiac disease autoimmunity}},
  year         = {{2021}},
}