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DivIVA is required for polar growth in the MreB-lacking rod-shaped actinomycete Corynebacterium glutamicum.

Letek, Michal; Ordóñez, Efrén; Vaquera, José; Margolin, William; Flärdh, Klas LU ; Mateos, Luis M and Gil, José A (2008) In Journal of Bacteriology 190. p.3283-3292
Abstract
The actinomycete Corynebacterium glutamicum grows as rod-shaped cells by zonal peptidoglycan synthesis at the cell poles. In this bacterium, experimental depletion of the polar DivIVACG protein resulted in the inhibition of polar growth; consequently, these cells exhibited a coccoid morphology. This result demonstrated that DivIVA is required for cell elongation and the acquisition of a rod shape. DivIVA from Streptomyces or Mycobacterium localized to the cell poles of DivIVACG-depleted C. glutamicum and restored polar peptidoglycan synthesis, in contrast to DivIVAs from Bacillus subtilis or Streptococcus pneumoniae which localized at the septum of C. glutamicum. This confirmed that DivIVAs from actinomycetes are involved in polarized cell... (More)
The actinomycete Corynebacterium glutamicum grows as rod-shaped cells by zonal peptidoglycan synthesis at the cell poles. In this bacterium, experimental depletion of the polar DivIVACG protein resulted in the inhibition of polar growth; consequently, these cells exhibited a coccoid morphology. This result demonstrated that DivIVA is required for cell elongation and the acquisition of a rod shape. DivIVA from Streptomyces or Mycobacterium localized to the cell poles of DivIVACG-depleted C. glutamicum and restored polar peptidoglycan synthesis, in contrast to DivIVAs from Bacillus subtilis or Streptococcus pneumoniae which localized at the septum of C. glutamicum. This confirmed that DivIVAs from actinomycetes are involved in polarized cell growth. DivIVACG localized at the septum after cell-wall synthesis had started and the nucleoids had already segregated, suggesting that in C. glutamicum DivIVA is not involved in cell division or chromosome segregation. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Bacteriology
volume
190
pages
3283 - 3292
publisher
American Society for Microbiology
external identifiers
  • pmid:18296522
  • wos:000255202100022
  • scopus:42549130004
ISSN
0021-9193
DOI
10.1128/JB.01934-07
language
English
LU publication?
yes
id
c6aac3e7-b522-4d09-b038-c57d7da5388e (old id 1041561)
date added to LUP
2008-03-11 13:58:37
date last changed
2017-09-10 03:35:18
@article{c6aac3e7-b522-4d09-b038-c57d7da5388e,
  abstract     = {The actinomycete Corynebacterium glutamicum grows as rod-shaped cells by zonal peptidoglycan synthesis at the cell poles. In this bacterium, experimental depletion of the polar DivIVACG protein resulted in the inhibition of polar growth; consequently, these cells exhibited a coccoid morphology. This result demonstrated that DivIVA is required for cell elongation and the acquisition of a rod shape. DivIVA from Streptomyces or Mycobacterium localized to the cell poles of DivIVACG-depleted C. glutamicum and restored polar peptidoglycan synthesis, in contrast to DivIVAs from Bacillus subtilis or Streptococcus pneumoniae which localized at the septum of C. glutamicum. This confirmed that DivIVAs from actinomycetes are involved in polarized cell growth. DivIVACG localized at the septum after cell-wall synthesis had started and the nucleoids had already segregated, suggesting that in C. glutamicum DivIVA is not involved in cell division or chromosome segregation.},
  author       = {Letek, Michal and Ordóñez, Efrén and Vaquera, José and Margolin, William and Flärdh, Klas and Mateos, Luis M and Gil, José A},
  issn         = {0021-9193},
  language     = {eng},
  pages        = {3283--3292},
  publisher    = {American Society for Microbiology},
  series       = {Journal of Bacteriology},
  title        = {DivIVA is required for polar growth in the MreB-lacking rod-shaped actinomycete Corynebacterium glutamicum.},
  url          = {http://dx.doi.org/10.1128/JB.01934-07},
  volume       = {190},
  year         = {2008},
}