DivIVA is required for polar growth in the MreB-lacking rod-shaped actinomycete Corynebacterium glutamicum.
(2008) In Journal of Bacteriology 190. p.3283-3292- Abstract
- The actinomycete Corynebacterium glutamicum grows as rod-shaped cells by zonal peptidoglycan synthesis at the cell poles. In this bacterium, experimental depletion of the polar DivIVACG protein resulted in the inhibition of polar growth; consequently, these cells exhibited a coccoid morphology. This result demonstrated that DivIVA is required for cell elongation and the acquisition of a rod shape. DivIVA from Streptomyces or Mycobacterium localized to the cell poles of DivIVACG-depleted C. glutamicum and restored polar peptidoglycan synthesis, in contrast to DivIVAs from Bacillus subtilis or Streptococcus pneumoniae which localized at the septum of C. glutamicum. This confirmed that DivIVAs from actinomycetes are involved in polarized cell... (More)
- The actinomycete Corynebacterium glutamicum grows as rod-shaped cells by zonal peptidoglycan synthesis at the cell poles. In this bacterium, experimental depletion of the polar DivIVACG protein resulted in the inhibition of polar growth; consequently, these cells exhibited a coccoid morphology. This result demonstrated that DivIVA is required for cell elongation and the acquisition of a rod shape. DivIVA from Streptomyces or Mycobacterium localized to the cell poles of DivIVACG-depleted C. glutamicum and restored polar peptidoglycan synthesis, in contrast to DivIVAs from Bacillus subtilis or Streptococcus pneumoniae which localized at the septum of C. glutamicum. This confirmed that DivIVAs from actinomycetes are involved in polarized cell growth. DivIVACG localized at the septum after cell-wall synthesis had started and the nucleoids had already segregated, suggesting that in C. glutamicum DivIVA is not involved in cell division or chromosome segregation. (Less)
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https://lup.lub.lu.se/record/1041561
- author
- Letek, Michal ; Ordóñez, Efrén ; Vaquera, José ; Margolin, William ; Flärdh, Klas LU ; Mateos, Luis M and Gil, José A
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Bacteriology
- volume
- 190
- pages
- 3283 - 3292
- publisher
- American Society for Microbiology
- external identifiers
-
- pmid:18296522
- wos:000255202100022
- scopus:42549130004
- pmid:18296522
- ISSN
- 0021-9193
- DOI
- 10.1128/JB.01934-07
- language
- English
- LU publication?
- yes
- id
- c6aac3e7-b522-4d09-b038-c57d7da5388e (old id 1041561)
- date added to LUP
- 2016-04-01 11:48:30
- date last changed
- 2024-05-07 15:57:59
@article{c6aac3e7-b522-4d09-b038-c57d7da5388e,
abstract = {{The actinomycete Corynebacterium glutamicum grows as rod-shaped cells by zonal peptidoglycan synthesis at the cell poles. In this bacterium, experimental depletion of the polar DivIVACG protein resulted in the inhibition of polar growth; consequently, these cells exhibited a coccoid morphology. This result demonstrated that DivIVA is required for cell elongation and the acquisition of a rod shape. DivIVA from Streptomyces or Mycobacterium localized to the cell poles of DivIVACG-depleted C. glutamicum and restored polar peptidoglycan synthesis, in contrast to DivIVAs from Bacillus subtilis or Streptococcus pneumoniae which localized at the septum of C. glutamicum. This confirmed that DivIVAs from actinomycetes are involved in polarized cell growth. DivIVACG localized at the septum after cell-wall synthesis had started and the nucleoids had already segregated, suggesting that in C. glutamicum DivIVA is not involved in cell division or chromosome segregation.}},
author = {{Letek, Michal and Ordóñez, Efrén and Vaquera, José and Margolin, William and Flärdh, Klas and Mateos, Luis M and Gil, José A}},
issn = {{0021-9193}},
language = {{eng}},
pages = {{3283--3292}},
publisher = {{American Society for Microbiology}},
series = {{Journal of Bacteriology}},
title = {{DivIVA is required for polar growth in the MreB-lacking rod-shaped actinomycete Corynebacterium glutamicum.}},
url = {{http://dx.doi.org/10.1128/JB.01934-07}},
doi = {{10.1128/JB.01934-07}},
volume = {{190}},
year = {{2008}},
}