The Antibacterial Chemokine MIG/CXCL9 Is Constitutively Expressed in Epithelial Cells of the Male Urogenital Tract and Is Present in Seminal Plasma.
Linge, Helena LU ; Collin, Mattias LU
; Giwercman, Aleksander
LU
; Malm, Johan
LU
; Bjartell, Anders
LU
and Egesten, Arne
LU
(2008)
In Journal of Interferon and Cytokine Research
28(3).
p.190-196
- Abstract
- ABSTRACT The integrity of the urogenital tract against potentially invasive pathogens is important for the health of the individual, fertilization, and continuance of species. Antibiotic peptides with broad antimicrobial activity, among them chemokines, are part of the innate immune system. We investigated the presence of the antibacterial interferon (IFN)-dependent CXC chemokines, MIG/CXCL9, IP-10/CXCL10, and I-TAC/CXCL11, in the human male reproductive system. MIG/CXCL9 was detected at 25.0 nM (range 8.1-40.6 nM; n = 14), whereas IP-10/CXCL10 and I-TAC/CXCL11 were detected at lower levels (mean 1.8 nM, range 0.3-5.8 nM and mean 0.6, 0.2-1.6 nM, respectively) in seminal plasma of fertile donors. The levels of MIG/CXCL9 are more than... (More)
- ABSTRACT The integrity of the urogenital tract against potentially invasive pathogens is important for the health of the individual, fertilization, and continuance of species. Antibiotic peptides with broad antimicrobial activity, among them chemokines, are part of the innate immune system. We investigated the presence of the antibacterial interferon (IFN)-dependent CXC chemokines, MIG/CXCL9, IP-10/CXCL10, and I-TAC/CXCL11, in the human male reproductive system. MIG/CXCL9 was detected at 25.0 nM (range 8.1-40.6 nM; n = 14), whereas IP-10/CXCL10 and I-TAC/CXCL11 were detected at lower levels (mean 1.8 nM, range 0.3-5.8 nM and mean 0.6, 0.2-1.6 nM, respectively) in seminal plasma of fertile donors. The levels of MIG/CXCL9 are more than 300-fold higher than those previously reported in blood plasma. In vasectomized donors, significantly lower levels of MIG/CXCL9 (mean 14.7 nM, range 6.6-21.8) were found, suggesting that the testis and epididymis, in addition to the prostate, significantly contribute to the MIG/CXCL9 content of seminal plasma. Strong expression of MIG/CXCL9 was found in the epithelium of testis, epididymis, and prostate, as detected by immunohistochemistry. MIG/CXCL9 at concentrations in the order of those found in seminal plasma possessed antibacterial activity against the urogenital pathogen Neisseria gonorrhoeae. The relatively high levels of MIG/CXCL9 in seminal plasma point to roles for this chemokine in both host defense of the male urogenital tract and during fertilization. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1052482
- author
- Linge, Helena
LU
; Collin, Mattias
LU
; Giwercman, Aleksander
LU
; Malm, Johan
LU
; Bjartell, Anders
LU
and Egesten, Arne
LU
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Interferon and Cytokine Research
- volume
- 28
- issue
- 3
- pages
- 190 - 196
- publisher
- Mary Ann Liebert, Inc.
- external identifiers
-
- pmid:18338951
- wos:000254301900006
- scopus:40749116459
- pmid:18338951
- ISSN
- 1079-9907
- DOI
- 10.1089/jir.2007.0100
- language
- English
- LU publication?
- yes
- id
- 54ffb45c-308a-4a99-82be-c67ded33ff3f (old id 1052482)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/18338951?dopt=Abstract
- date added to LUP
- 2016-04-04 09:18:59
- date last changed
- 2022-01-29 17:16:57
@article{54ffb45c-308a-4a99-82be-c67ded33ff3f,
abstract = {{ABSTRACT The integrity of the urogenital tract against potentially invasive pathogens is important for the health of the individual, fertilization, and continuance of species. Antibiotic peptides with broad antimicrobial activity, among them chemokines, are part of the innate immune system. We investigated the presence of the antibacterial interferon (IFN)-dependent CXC chemokines, MIG/CXCL9, IP-10/CXCL10, and I-TAC/CXCL11, in the human male reproductive system. MIG/CXCL9 was detected at 25.0 nM (range 8.1-40.6 nM; n = 14), whereas IP-10/CXCL10 and I-TAC/CXCL11 were detected at lower levels (mean 1.8 nM, range 0.3-5.8 nM and mean 0.6, 0.2-1.6 nM, respectively) in seminal plasma of fertile donors. The levels of MIG/CXCL9 are more than 300-fold higher than those previously reported in blood plasma. In vasectomized donors, significantly lower levels of MIG/CXCL9 (mean 14.7 nM, range 6.6-21.8) were found, suggesting that the testis and epididymis, in addition to the prostate, significantly contribute to the MIG/CXCL9 content of seminal plasma. Strong expression of MIG/CXCL9 was found in the epithelium of testis, epididymis, and prostate, as detected by immunohistochemistry. MIG/CXCL9 at concentrations in the order of those found in seminal plasma possessed antibacterial activity against the urogenital pathogen Neisseria gonorrhoeae. The relatively high levels of MIG/CXCL9 in seminal plasma point to roles for this chemokine in both host defense of the male urogenital tract and during fertilization.}},
author = {{Linge, Helena and Collin, Mattias and Giwercman, Aleksander and Malm, Johan and Bjartell, Anders and Egesten, Arne}},
issn = {{1079-9907}},
language = {{eng}},
number = {{3}},
pages = {{190--196}},
publisher = {{Mary Ann Liebert, Inc.}},
series = {{Journal of Interferon and Cytokine Research}},
title = {{The Antibacterial Chemokine MIG/CXCL9 Is Constitutively Expressed in Epithelial Cells of the Male Urogenital Tract and Is Present in Seminal Plasma.}},
url = {{http://dx.doi.org/10.1089/jir.2007.0100}},
doi = {{10.1089/jir.2007.0100}},
volume = {{28}},
year = {{2008}},
}