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Role of mast cells in the development of pancreatitis-induced multiple organ dysfunction.

Dib, Marwan LU ; Zhao, Xia LU ; Wang, X D and Andersson, Roland LU (2002) In British Journal of Surgery 89(2). p.172-178
Abstract
BACKGROUND: Activated mast cells can produce and release a number of inflammatory mediators involved in the pathophysiology of acute conditions. The aim of the present study was to evaluate the role of activated tissue mast cells in the pathogenesis of multiple organ dysfunction syndrome following acute pancreatitis (AP). METHODS: AP was induced by the intraductal infusion of 5 per cent sodium taurodeoxycholate in the rat. Some 30 min before induction of AP, a mast cell stabilizer (sodium cromoglycate (SCG)) or antihistamines (pyrilamine, cyproheptadine, meclizine and amitriptyline) were administered intra peritoneally. Plasma exudation of radiolabelled albumin, histamine, myeloperoxidase (MPO), monocyte chemoattractant protein (MCP) 1 and... (More)
BACKGROUND: Activated mast cells can produce and release a number of inflammatory mediators involved in the pathophysiology of acute conditions. The aim of the present study was to evaluate the role of activated tissue mast cells in the pathogenesis of multiple organ dysfunction syndrome following acute pancreatitis (AP). METHODS: AP was induced by the intraductal infusion of 5 per cent sodium taurodeoxycholate in the rat. Some 30 min before induction of AP, a mast cell stabilizer (sodium cromoglycate (SCG)) or antihistamines (pyrilamine, cyproheptadine, meclizine and amitriptyline) were administered intra peritoneally. Plasma exudation of radiolabelled albumin, histamine, myeloperoxidase (MPO), monocyte chemoattractant protein (MCP) 1 and adhesion molecules (platelet endothelial cell adhesion molecule (PECAM) 1 and L-selectin) were measured. RESULTS: The mast cell stabilizer significantly reduced plasma exudation in the pancreas, colon and lungs (P < 0.05), decreased the release of histamine at 1 h (P < 0.05), and reduced MPO activity and MCP-1 levels in the colon and lungs (P < 0.05) but not in the pancreas. Expression of PECAM-1 and L-selectin on total circulating leucocytes in rats with AP and SCG pretreatment did not differ from that in sham controls, while levels in animals that had AP and saline pretreatment were half of those seen following sham operation. CONCLUSION: Activation of mast cells after induction of AP is involved in the development of endothelial barrier dysfunction in both the pancreas and extrapancreatic organs/tissues, particularly in the lungs and colon. This may, at least partly, contribute to the sequential development of multiple organ dysfunction and organ/tissue-specific endothelial barrier dysfunction. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Histamine/analysis, L-Selectin/metabolism, Leukocytes/physiology, Monocyte Chemoattractant Protein-1/analysis, Tumor Necrosis Factor/analysis, Multiple Organ Failure, Pancreatitis, Flow Cytometry, Endothelium, CD31, Antigens, Acute Disease
in
British Journal of Surgery
volume
89
issue
2
pages
172 - 178
publisher
John Wiley & Sons
external identifiers
  • wos:000173865000008
  • pmid:11856129
  • scopus:0036159586
ISSN
1365-2168
DOI
10.1046/j.1365-2168.2002.01991.x
language
English
LU publication?
yes
id
8568031f-86a2-4524-8f69-839f0923d0c1 (old id 105877)
date added to LUP
2007-07-24 14:07:09
date last changed
2017-12-10 03:46:55
@article{8568031f-86a2-4524-8f69-839f0923d0c1,
  abstract     = {BACKGROUND: Activated mast cells can produce and release a number of inflammatory mediators involved in the pathophysiology of acute conditions. The aim of the present study was to evaluate the role of activated tissue mast cells in the pathogenesis of multiple organ dysfunction syndrome following acute pancreatitis (AP). METHODS: AP was induced by the intraductal infusion of 5 per cent sodium taurodeoxycholate in the rat. Some 30 min before induction of AP, a mast cell stabilizer (sodium cromoglycate (SCG)) or antihistamines (pyrilamine, cyproheptadine, meclizine and amitriptyline) were administered intra peritoneally. Plasma exudation of radiolabelled albumin, histamine, myeloperoxidase (MPO), monocyte chemoattractant protein (MCP) 1 and adhesion molecules (platelet endothelial cell adhesion molecule (PECAM) 1 and L-selectin) were measured. RESULTS: The mast cell stabilizer significantly reduced plasma exudation in the pancreas, colon and lungs (P &lt; 0.05), decreased the release of histamine at 1 h (P &lt; 0.05), and reduced MPO activity and MCP-1 levels in the colon and lungs (P &lt; 0.05) but not in the pancreas. Expression of PECAM-1 and L-selectin on total circulating leucocytes in rats with AP and SCG pretreatment did not differ from that in sham controls, while levels in animals that had AP and saline pretreatment were half of those seen following sham operation. CONCLUSION: Activation of mast cells after induction of AP is involved in the development of endothelial barrier dysfunction in both the pancreas and extrapancreatic organs/tissues, particularly in the lungs and colon. This may, at least partly, contribute to the sequential development of multiple organ dysfunction and organ/tissue-specific endothelial barrier dysfunction.},
  author       = {Dib, Marwan and Zhao, Xia and Wang, X D and Andersson, Roland},
  issn         = {1365-2168},
  keyword      = {Histamine/analysis,L-Selectin/metabolism,Leukocytes/physiology,Monocyte Chemoattractant Protein-1/analysis,Tumor Necrosis Factor/analysis,Multiple Organ Failure,Pancreatitis,Flow Cytometry,Endothelium,CD31,Antigens,Acute Disease},
  language     = {eng},
  number       = {2},
  pages        = {172--178},
  publisher    = {John Wiley & Sons},
  series       = {British Journal of Surgery},
  title        = {Role of mast cells in the development of pancreatitis-induced multiple organ dysfunction.},
  url          = {http://dx.doi.org/10.1046/j.1365-2168.2002.01991.x},
  volume       = {89},
  year         = {2002},
}