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Gender difference in the glucagon response to glucopenic stress in mice.

Karlsson, Sven; Scheurink, Anton J W and Ahrén, Bo LU (2002) In American Journal of Physiology: Regulatory, Integrative and Comparative Physiology 282(1). p.281-288
Abstract
A gender difference in the glucagon response to insulin-induced hypoglycemia was previously demonstrated in humans. Whether this reflects a gender difference in autonomic activation or in pancreatic alpha-cell regulation is not known. We investigated the glucagon, epinephrine, and norepinephrine responses to neuroglycopenic stress induced by 2-deoxy-D-glucose (2-DG) or insulin in female and male mice. 2-DG increased plasma glucagon levels by 559 +/- 68% in females versus 281 +/- 46% in males (P < 0.01). Plasma levels of epinephrine or norepinephrine after 2-DG administration did not differ between genders. During insulin-induced hypoglycemia, the glucagon response was similarly higher in females (P < 0.001), whereas the plasma... (More)
A gender difference in the glucagon response to insulin-induced hypoglycemia was previously demonstrated in humans. Whether this reflects a gender difference in autonomic activation or in pancreatic alpha-cell regulation is not known. We investigated the glucagon, epinephrine, and norepinephrine responses to neuroglycopenic stress induced by 2-deoxy-D-glucose (2-DG) or insulin in female and male mice. 2-DG increased plasma glucagon levels by 559 +/- 68% in females versus 281 +/- 46% in males (P < 0.01). Plasma levels of epinephrine or norepinephrine after 2-DG administration did not differ between genders. During insulin-induced hypoglycemia, the glucagon response was similarly higher in females (P < 0.001), whereas the plasma catecholamine response was higher in males (P < 0.05). In vivo, the glucagon response to carbachol or clonidine was higher in females (P < 0.05). In isolated islets, the glucagon response to carbachol (100 microM; P = 0.003) but not to clonidine (1 microM) was larger in females. We conclude that in addition to a larger alpha-cell mass (previously described in female mice), an increased sensitivity of the glucagon-producing alpha-cell to cholinergic activation contributes to the larger glucagon response to glucopenic stress in female mice. (Less)
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Sympatholytics : pharmacology, Stress : metabolism, Sex Characteristics, Norepinephrine : blood, Mice Inbred Strains, Mice, Male, Islets of Langerhans : drug effects : physiology : secretion, Insulin : pharmacology, Hypoglycemic Agents : pharmacology, Female, Glucagon : blood : secretion, Hypoglycemia : chemically induced : metabolism, Epinephrine : blood, Clonidine : pharmacology, Deoxyglucose : pharmacology, Support Non-U.S. Gov't, Cholinergic Agonists : pharmacology, Carbachol : pharmacology, Blood Glucose : metabolism, Autonomic Nervous System : metabolism, Antimetabolites : pharmacology, Animal, Adrenal Glands : physiology
in
American Journal of Physiology: Regulatory, Integrative and Comparative Physiology
volume
282
issue
1
pages
281 - 288
publisher
American Physiological Society
external identifiers
  • wos:000172722000034
  • pmid:11742849
  • scopus:0036083645
ISSN
0363-6119
language
English
LU publication?
yes
id
437ec404-89c8-4b0e-b1ca-cd4b1d535554 (old id 107024)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11742849&dopt=Abstract
http://ajpregu.physiology.org/cgi/content/full/282/1/R281
date added to LUP
2007-06-29 11:37:46
date last changed
2017-01-01 07:24:00
@article{437ec404-89c8-4b0e-b1ca-cd4b1d535554,
  abstract     = {A gender difference in the glucagon response to insulin-induced hypoglycemia was previously demonstrated in humans. Whether this reflects a gender difference in autonomic activation or in pancreatic alpha-cell regulation is not known. We investigated the glucagon, epinephrine, and norepinephrine responses to neuroglycopenic stress induced by 2-deoxy-D-glucose (2-DG) or insulin in female and male mice. 2-DG increased plasma glucagon levels by 559 +/- 68% in females versus 281 +/- 46% in males (P &lt; 0.01). Plasma levels of epinephrine or norepinephrine after 2-DG administration did not differ between genders. During insulin-induced hypoglycemia, the glucagon response was similarly higher in females (P &lt; 0.001), whereas the plasma catecholamine response was higher in males (P &lt; 0.05). In vivo, the glucagon response to carbachol or clonidine was higher in females (P &lt; 0.05). In isolated islets, the glucagon response to carbachol (100 microM; P = 0.003) but not to clonidine (1 microM) was larger in females. We conclude that in addition to a larger alpha-cell mass (previously described in female mice), an increased sensitivity of the glucagon-producing alpha-cell to cholinergic activation contributes to the larger glucagon response to glucopenic stress in female mice.},
  author       = {Karlsson, Sven and Scheurink, Anton J W and Ahrén, Bo},
  issn         = {0363-6119},
  keyword      = {Sympatholytics : pharmacology,Stress : metabolism,Sex Characteristics,Norepinephrine : blood,Mice Inbred Strains,Mice,Male,Islets of Langerhans : drug effects : physiology : secretion,Insulin : pharmacology,Hypoglycemic Agents : pharmacology,Female,Glucagon : blood : secretion,Hypoglycemia : chemically induced : metabolism,Epinephrine : blood,Clonidine : pharmacology,Deoxyglucose : pharmacology,Support Non-U.S. Gov't,Cholinergic Agonists : pharmacology,Carbachol : pharmacology,Blood Glucose : metabolism,Autonomic Nervous System : metabolism,Antimetabolites : pharmacology,Animal,Adrenal Glands : physiology},
  language     = {eng},
  number       = {1},
  pages        = {281--288},
  publisher    = {American Physiological Society},
  series       = {American Journal of Physiology: Regulatory, Integrative and Comparative Physiology},
  title        = {Gender difference in the glucagon response to glucopenic stress in mice.},
  volume       = {282},
  year         = {2002},
}