Detection of Escherichia coli in water by culture-based amperometric and luminometric methods.
(2002) In Water Science and Technology 45(4-5). p.191-199- Abstract
- The application of amperometric biosensor- and chemiluminiscence based methods for rapid detection of viable E. coli in water has been investigated. An amplification of the amperometric signal by a factor of 4 was obtained when the cellobiose dehydrogenase (CDH) biosensor was used instead of a plain graphite electrode for detection of b-galactosidase (b-GAL) activity at 22.5 degrees C. A linear correlation was demonstrated for detection time (DT) vs. initial concentrations (logarithmic units) of E. coli IT1 and E. coli in environmental samples, respectively, by use of the CDH biosensor or a chemiluminometric technique. The study has shown that an E. coli concentration > or = 10(4) cfu/100 mL in environmental samples was determined by... (More)
- The application of amperometric biosensor- and chemiluminiscence based methods for rapid detection of viable E. coli in water has been investigated. An amplification of the amperometric signal by a factor of 4 was obtained when the cellobiose dehydrogenase (CDH) biosensor was used instead of a plain graphite electrode for detection of b-galactosidase (b-GAL) activity at 22.5 degrees C. A linear correlation was demonstrated for detection time (DT) vs. initial concentrations (logarithmic units) of E. coli IT1 and E. coli in environmental samples, respectively, by use of the CDH biosensor or a chemiluminometric technique. The study has shown that an E. coli concentration > or = 10(4) cfu/100 mL in environmental samples was determined by the CDH biosensor within one working day. However, further reduction of the DT can be obtained, e.g. by increasing the signal amplification factor using other biosensors. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/107445
- author
- Nistor, Catalin LU ; Osvik, A ; Davidsson, Richard LU ; Rose, A ; Wollenberger, U ; Pfeiffer, D ; Emnéus, Jenny LU and Fiksdal, L
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Water Science and Technology
- volume
- 45
- issue
- 4-5
- pages
- 191 - 199
- publisher
- IWA Publishing
- external identifiers
-
- pmid:11936634
- wos:000174872000025
- scopus:0036206135
- ISSN
- 0273-1223
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
- id
- dc42a5bc-dffd-4350-a7f5-7ebfbadc1153 (old id 107445)
- alternative location
- http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11936634&dopt=Abstract
- date added to LUP
- 2016-04-01 16:29:30
- date last changed
- 2022-02-12 22:29:44
@article{dc42a5bc-dffd-4350-a7f5-7ebfbadc1153,
abstract = {{The application of amperometric biosensor- and chemiluminiscence based methods for rapid detection of viable E. coli in water has been investigated. An amplification of the amperometric signal by a factor of 4 was obtained when the cellobiose dehydrogenase (CDH) biosensor was used instead of a plain graphite electrode for detection of b-galactosidase (b-GAL) activity at 22.5 degrees C. A linear correlation was demonstrated for detection time (DT) vs. initial concentrations (logarithmic units) of E. coli IT1 and E. coli in environmental samples, respectively, by use of the CDH biosensor or a chemiluminometric technique. The study has shown that an E. coli concentration > or = 10(4) cfu/100 mL in environmental samples was determined by the CDH biosensor within one working day. However, further reduction of the DT can be obtained, e.g. by increasing the signal amplification factor using other biosensors.}},
author = {{Nistor, Catalin and Osvik, A and Davidsson, Richard and Rose, A and Wollenberger, U and Pfeiffer, D and Emnéus, Jenny and Fiksdal, L}},
issn = {{0273-1223}},
language = {{eng}},
number = {{4-5}},
pages = {{191--199}},
publisher = {{IWA Publishing}},
series = {{Water Science and Technology}},
title = {{Detection of Escherichia coli in water by culture-based amperometric and luminometric methods.}},
url = {{http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11936634&dopt=Abstract}},
volume = {{45}},
year = {{2002}},
}