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Detection of Escherichia coli in water by culture-based amperometric and luminometric methods.

Nistor, Catalin LU ; Osvik, A; Davidsson, Richard LU ; Rose, A; Wollenberger, U; Pfeiffer, D; Emnéus, Jenny LU and Fiksdal, L (2002) In Water Science and Technology 45(4-5). p.191-199
Abstract
The application of amperometric biosensor- and chemiluminiscence based methods for rapid detection of viable E. coli in water has been investigated. An amplification of the amperometric signal by a factor of 4 was obtained when the cellobiose dehydrogenase (CDH) biosensor was used instead of a plain graphite electrode for detection of b-galactosidase (b-GAL) activity at 22.5 degrees C. A linear correlation was demonstrated for detection time (DT) vs. initial concentrations (logarithmic units) of E. coli IT1 and E. coli in environmental samples, respectively, by use of the CDH biosensor or a chemiluminometric technique. The study has shown that an E. coli concentration > or = 10(4) cfu/100 mL in environmental samples was determined by... (More)
The application of amperometric biosensor- and chemiluminiscence based methods for rapid detection of viable E. coli in water has been investigated. An amplification of the amperometric signal by a factor of 4 was obtained when the cellobiose dehydrogenase (CDH) biosensor was used instead of a plain graphite electrode for detection of b-galactosidase (b-GAL) activity at 22.5 degrees C. A linear correlation was demonstrated for detection time (DT) vs. initial concentrations (logarithmic units) of E. coli IT1 and E. coli in environmental samples, respectively, by use of the CDH biosensor or a chemiluminometric technique. The study has shown that an E. coli concentration > or = 10(4) cfu/100 mL in environmental samples was determined by the CDH biosensor within one working day. However, further reduction of the DT can be obtained, e.g. by increasing the signal amplification factor using other biosensors. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Water Science and Technology
volume
45
issue
4-5
pages
191 - 199
publisher
IWA Publishing
external identifiers
  • pmid:11936634
  • wos:000174872000025
  • scopus:0036206135
ISSN
0273-1223
language
English
LU publication?
yes
id
dc42a5bc-dffd-4350-a7f5-7ebfbadc1153 (old id 107445)
alternative location
http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11936634&dopt=Abstract
date added to LUP
2007-06-27 13:36:52
date last changed
2017-05-21 04:23:39
@article{dc42a5bc-dffd-4350-a7f5-7ebfbadc1153,
  abstract     = {The application of amperometric biosensor- and chemiluminiscence based methods for rapid detection of viable E. coli in water has been investigated. An amplification of the amperometric signal by a factor of 4 was obtained when the cellobiose dehydrogenase (CDH) biosensor was used instead of a plain graphite electrode for detection of b-galactosidase (b-GAL) activity at 22.5 degrees C. A linear correlation was demonstrated for detection time (DT) vs. initial concentrations (logarithmic units) of E. coli IT1 and E. coli in environmental samples, respectively, by use of the CDH biosensor or a chemiluminometric technique. The study has shown that an E. coli concentration > or = 10(4) cfu/100 mL in environmental samples was determined by the CDH biosensor within one working day. However, further reduction of the DT can be obtained, e.g. by increasing the signal amplification factor using other biosensors.},
  author       = {Nistor, Catalin and Osvik, A and Davidsson, Richard and Rose, A and Wollenberger, U and Pfeiffer, D and Emnéus, Jenny and Fiksdal, L},
  issn         = {0273-1223},
  language     = {eng},
  number       = {4-5},
  pages        = {191--199},
  publisher    = {IWA Publishing},
  series       = {Water Science and Technology},
  title        = {Detection of Escherichia coli in water by culture-based amperometric and luminometric methods.},
  volume       = {45},
  year         = {2002},
}