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Mechanical function of intermediate filaments in arteries of different size examined using desmin deficient mice.

Karlsson Wede, Oskar LU ; Löfgren, Mia; Li, Zhenlin; Paulin, Denise and Arner, Anders LU (2002) In Journal of Physiology 540(Pt 3). p.941-949
Abstract
Protein composition and mechanical function of intermediate filaments were examined in arteries of different sizes using desmin deficient mice (Des-/-) and their wild-type controls (Des+/+). Using SDS-PAGE gels and Western blots we found a gradient in desmin expression in the arterial tree; the desmin content increased from the elastic artery aorta, via the muscular mesenteric artery to the resistance-sized mesenteric microarteries approximately 150 microm in diameter in Des+/+ mice. Mechanical experiments were performed on the aorta, the mesenteric artery and resistance-sized arteries using wire myographs. For aorta and mesenteric artery, no differences in passive or active circumference- stress relations were found between Des-/- and... (More)
Protein composition and mechanical function of intermediate filaments were examined in arteries of different sizes using desmin deficient mice (Des-/-) and their wild-type controls (Des+/+). Using SDS-PAGE gels and Western blots we found a gradient in desmin expression in the arterial tree; the desmin content increased from the elastic artery aorta, via the muscular mesenteric artery to the resistance-sized mesenteric microarteries approximately 150 microm in diameter in Des+/+ mice. Mechanical experiments were performed on the aorta, the mesenteric artery and resistance-sized arteries using wire myographs. For aorta and mesenteric artery, no differences in passive or active circumference- stress relations were found between Des-/- and Des+/+ mice. In microarteries, both passive and active stress were lower in the Des-/- group. In conclusion, large elastic and muscular arteries contain a relatively low amount of desmin, and the desmin intermediate filaments do not seem to play a major role in the mechanical properties of these larger arterial vessels. In the microarteries, where expression of desmin is high, desmin plays a role in supporting both passive and active tension. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Physiology
volume
540
issue
Pt 3
pages
941 - 949
publisher
The Physiological Society
external identifiers
  • wos:000175626300019
  • pmid:11986381
  • scopus:0036588248
ISSN
1469-7793
DOI
10.1113/jphysiol.2001.014910
language
English
LU publication?
yes
id
41e8c845-e281-4dec-9879-50cdbbb2bda9 (old id 107958)
date added to LUP
2007-07-16 11:34:19
date last changed
2017-10-22 04:38:54
@article{41e8c845-e281-4dec-9879-50cdbbb2bda9,
  abstract     = {Protein composition and mechanical function of intermediate filaments were examined in arteries of different sizes using desmin deficient mice (Des-/-) and their wild-type controls (Des+/+). Using SDS-PAGE gels and Western blots we found a gradient in desmin expression in the arterial tree; the desmin content increased from the elastic artery aorta, via the muscular mesenteric artery to the resistance-sized mesenteric microarteries approximately 150 microm in diameter in Des+/+ mice. Mechanical experiments were performed on the aorta, the mesenteric artery and resistance-sized arteries using wire myographs. For aorta and mesenteric artery, no differences in passive or active circumference- stress relations were found between Des-/- and Des+/+ mice. In microarteries, both passive and active stress were lower in the Des-/- group. In conclusion, large elastic and muscular arteries contain a relatively low amount of desmin, and the desmin intermediate filaments do not seem to play a major role in the mechanical properties of these larger arterial vessels. In the microarteries, where expression of desmin is high, desmin plays a role in supporting both passive and active tension.},
  author       = {Karlsson Wede, Oskar and Löfgren, Mia and Li, Zhenlin and Paulin, Denise and Arner, Anders},
  issn         = {1469-7793},
  language     = {eng},
  number       = {Pt 3},
  pages        = {941--949},
  publisher    = {The Physiological Society},
  series       = {Journal of Physiology},
  title        = {Mechanical function of intermediate filaments in arteries of different size examined using desmin deficient mice.},
  url          = {http://dx.doi.org/10.1113/jphysiol.2001.014910},
  volume       = {540},
  year         = {2002},
}