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Enhanced efficacy of porcine lung surfactant extract by utilization of its aqueous swelling dynamics.

Larsson, Marcus; Haitsma, Jack J; Lachmann, Burkhard; Larsson, Kåre; Nylander, Tommy LU and Wollmer, Per LU (2002) In Clinical Physiology and Functional Imaging2002-01-01+01:00 22(1). p.39-48
Abstract
This study investigates the interactions between a porcine lung surfactant (PLS) extract and distilled water, saline solution or Ringer solution. The phases which coexist in equilibrium with water or electrolyte solutions were analysed by X-ray diffraction and cryo transmission electron microscopy (cryo-TEM). A lamellar phase with a structure unit consisting of double bilayers was observed in water, whereas lamellar phases with the usual bilayer structure unit were formed in saline and in Ringer solutions. At 25 degrees C the presence of a 4.2-A peak in the X-ray diffraction wide-angle region of these three maximally swollen phases showed that most of the hydrocarbon chains were organized in a crystalline packing. At 42 degrees C the... (More)
This study investigates the interactions between a porcine lung surfactant (PLS) extract and distilled water, saline solution or Ringer solution. The phases which coexist in equilibrium with water or electrolyte solutions were analysed by X-ray diffraction and cryo transmission electron microscopy (cryo-TEM). A lamellar phase with a structure unit consisting of double bilayers was observed in water, whereas lamellar phases with the usual bilayer structure unit were formed in saline and in Ringer solutions. At 25 degrees C the presence of a 4.2-A peak in the X-ray diffraction wide-angle region of these three maximally swollen phases showed that most of the hydrocarbon chains were organized in a crystalline packing. At 42 degrees C the chains in all three phases were melted which, in combination with the low-angle diffraction, shows that they were liquid-crystalline. Polyhedral-like vesicles and spherically shaped multilamellar vesicles were observed in cryo-TEM. The bilayer unit structures were consistent with the periodicity seen by X-ray diffraction. The dynamic swelling behaviour was followed in the polarizing microscope. A remarkable growth of birefringent networks was seen at the air interface of samples swollen in Ringer solution and saline solution. No such interfacial growth phenomena were observed during swelling in water without electrolytes. Then, these dynamics were analysed in relation to time-dependent pulmonary administration of the surfactant extract in rats. Variation in the time of administration (20 and 60 min) after mixing the extract with saline or Ringer solution showed clear differences in physiological effects. At pulmonary administration when the swelling behaviour in vitro showed a maximum in dynamics, the arterial oxygenation was superior to that of administration at a time after a steady-state had been reached. This means that the clinical performance of mammalian lung surfactant extracts can be significantly improved by taking the time-dependent aqueous swelling of the extract into account. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Tissue Extracts : pharmacology, Swine, Non-U.S. Gov't, Solutions, Support, Sodium Chloride, Pulmonary Surfactants : chemistry, Pulmonary Surfactants : pharmacology, Pulmonary Surfactants : physiology, Pulmonary Surfactants : ultrastructure, Rats, Sprague-Dawley, Polarization, Microscopy, Electron, Male, Water, Lung : chemistry, Lung : drug effects, Isotonic Solutions, Freezing, Animal, Electrolytes
in
Clinical Physiology and Functional Imaging2002-01-01+01:00
volume
22
issue
1
pages
39 - 48
publisher
Wiley Online Library
external identifiers
  • wos:000174885800010
  • pmid:12003098
  • scopus:0036252298
ISSN
1475-0961
DOI
10.1046/j.1475-097X.2002.00397.x
language
English
LU publication?
yes
id
602c3106-737d-4ff2-ab72-b9d19c622a55 (old id 108124)
date added to LUP
2007-07-25 10:29:18
date last changed
2017-05-21 03:42:31
@article{602c3106-737d-4ff2-ab72-b9d19c622a55,
  abstract     = {This study investigates the interactions between a porcine lung surfactant (PLS) extract and distilled water, saline solution or Ringer solution. The phases which coexist in equilibrium with water or electrolyte solutions were analysed by X-ray diffraction and cryo transmission electron microscopy (cryo-TEM). A lamellar phase with a structure unit consisting of double bilayers was observed in water, whereas lamellar phases with the usual bilayer structure unit were formed in saline and in Ringer solutions. At 25 degrees C the presence of a 4.2-A peak in the X-ray diffraction wide-angle region of these three maximally swollen phases showed that most of the hydrocarbon chains were organized in a crystalline packing. At 42 degrees C the chains in all three phases were melted which, in combination with the low-angle diffraction, shows that they were liquid-crystalline. Polyhedral-like vesicles and spherically shaped multilamellar vesicles were observed in cryo-TEM. The bilayer unit structures were consistent with the periodicity seen by X-ray diffraction. The dynamic swelling behaviour was followed in the polarizing microscope. A remarkable growth of birefringent networks was seen at the air interface of samples swollen in Ringer solution and saline solution. No such interfacial growth phenomena were observed during swelling in water without electrolytes. Then, these dynamics were analysed in relation to time-dependent pulmonary administration of the surfactant extract in rats. Variation in the time of administration (20 and 60 min) after mixing the extract with saline or Ringer solution showed clear differences in physiological effects. At pulmonary administration when the swelling behaviour in vitro showed a maximum in dynamics, the arterial oxygenation was superior to that of administration at a time after a steady-state had been reached. This means that the clinical performance of mammalian lung surfactant extracts can be significantly improved by taking the time-dependent aqueous swelling of the extract into account.},
  author       = {Larsson, Marcus and Haitsma, Jack J and Lachmann, Burkhard and Larsson, Kåre and Nylander, Tommy and Wollmer, Per},
  issn         = {1475-0961},
  keyword      = {Tissue Extracts : pharmacology,Swine,Non-U.S. Gov't,Solutions,Support,Sodium Chloride,Pulmonary Surfactants : chemistry,Pulmonary Surfactants : pharmacology,Pulmonary Surfactants : physiology,Pulmonary Surfactants : ultrastructure,Rats,Sprague-Dawley,Polarization,Microscopy,Electron,Male,Water,Lung : chemistry,Lung : drug effects,Isotonic Solutions,Freezing,Animal,Electrolytes},
  language     = {eng},
  number       = {1},
  pages        = {39--48},
  publisher    = {Wiley Online Library},
  series       = {Clinical Physiology and Functional Imaging2002-01-01+01:00},
  title        = {Enhanced efficacy of porcine lung surfactant extract by utilization of its aqueous swelling dynamics.},
  url          = {http://dx.doi.org/10.1046/j.1475-097X.2002.00397.x},
  volume       = {22},
  year         = {2002},
}