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Separation and surveys of proteins of Helicobacter pylori.

Nilsson, Ingrid LU and Utt, Meeme LU (2002) In Journal of Chromatography. B 771(1-2). p.251-260
Abstract
The analysis of Helicobacter pylori proteins is a demanding task for the elucidation of virulence factors, antigens and vaccines, all important for diagnosis, therapy and protection. In the "pre-genomic era" the purification of proteins was mostly performed by using various techniques such as detergent treatment of the bacterial cells, ultra-centrifugation, various chromatographic methods, antibody detection, N-terminal sequence determination and finally cloning and identification of the corresponding gene. In this review, the most representative methods used for purification, separation and identification of H. pylori proteins will be presented as well as some important developments in the "post-genomic era" that have improved the... (More)
The analysis of Helicobacter pylori proteins is a demanding task for the elucidation of virulence factors, antigens and vaccines, all important for diagnosis, therapy and protection. In the "pre-genomic era" the purification of proteins was mostly performed by using various techniques such as detergent treatment of the bacterial cells, ultra-centrifugation, various chromatographic methods, antibody detection, N-terminal sequence determination and finally cloning and identification of the corresponding gene. In this review, the most representative methods used for purification, separation and identification of H. pylori proteins will be presented as well as some important developments in the "post-genomic era" that have improved the performance of these characterisation techniques. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Helicobacter pylori : chemistry, Bacterial, Genome, Two-Dimensional, Gel, Electrophoresis, Bacterial Proteins : isolation & purification, Bacterial Proteins : genetics, Proteome, Helicobacter pylori : genetics, Helicobacter pylori : pathogenicity
in
Journal of Chromatography. B
volume
771
issue
1-2
pages
251 - 260
publisher
Elsevier
external identifiers
  • wos:000175952300015
  • pmid:12016003
  • scopus:0037023870
ISSN
1873-376X
DOI
10.1016/S1570-0232(02)00113-7
language
English
LU publication?
yes
id
af09504f-98d8-4fc2-b733-5ad2c30ff052 (old id 108286)
alternative location
http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12016003&dopt=Abstract
date added to LUP
2007-07-10 08:53:12
date last changed
2017-01-01 05:11:33
@article{af09504f-98d8-4fc2-b733-5ad2c30ff052,
  abstract     = {The analysis of Helicobacter pylori proteins is a demanding task for the elucidation of virulence factors, antigens and vaccines, all important for diagnosis, therapy and protection. In the "pre-genomic era" the purification of proteins was mostly performed by using various techniques such as detergent treatment of the bacterial cells, ultra-centrifugation, various chromatographic methods, antibody detection, N-terminal sequence determination and finally cloning and identification of the corresponding gene. In this review, the most representative methods used for purification, separation and identification of H. pylori proteins will be presented as well as some important developments in the "post-genomic era" that have improved the performance of these characterisation techniques.},
  author       = {Nilsson, Ingrid and Utt, Meeme},
  issn         = {1873-376X},
  keyword      = {Helicobacter pylori : chemistry,Bacterial,Genome,Two-Dimensional,Gel,Electrophoresis,Bacterial Proteins : isolation & purification,Bacterial Proteins : genetics,Proteome,Helicobacter pylori : genetics,Helicobacter pylori : pathogenicity},
  language     = {eng},
  number       = {1-2},
  pages        = {251--260},
  publisher    = {Elsevier},
  series       = {Journal of Chromatography. B},
  title        = {Separation and surveys of proteins of Helicobacter pylori.},
  url          = {http://dx.doi.org/10.1016/S1570-0232(02)00113-7},
  volume       = {771},
  year         = {2002},
}