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Proteoglycan production in disomic and trisomy 7-carrying human synovial cells.

Eklund, Erik LU ; Broberg, Karin LU ; Westergren-Thorsson, Gunilla LU ; Bjärdahlen, Anette LU ; Hedlund, Maria LU and Malmström, Anders LU (2002) In Matrix Biology 21(4). p.325-335
Abstract
To gain further insight into the synthesis and structure of the synovial matrix of joints, we have established cell cultures from synovial specimens and elaborated their production of hyaluronan and proteoglycans. The cultures secreted mainly the small proteoglycan decorin, but also considerable amounts of the related biglycan and the large proteoglycan versican. Only minor amounts of heparan sulfate proteoglycans were found. All cultures also had a high production of hyaluronan, which highlights the important role for normal joint function of these cells. In joint diseases, a common feature is the presence of an extra chromosome 7 (trisomy 7) in the synovial cells. To study the possible consequences of trisomy 7 on the synovial cell... (More)
To gain further insight into the synthesis and structure of the synovial matrix of joints, we have established cell cultures from synovial specimens and elaborated their production of hyaluronan and proteoglycans. The cultures secreted mainly the small proteoglycan decorin, but also considerable amounts of the related biglycan and the large proteoglycan versican. Only minor amounts of heparan sulfate proteoglycans were found. All cultures also had a high production of hyaluronan, which highlights the important role for normal joint function of these cells. In joint diseases, a common feature is the presence of an extra chromosome 7 (trisomy 7) in the synovial cells. To study the possible consequences of trisomy 7 on the synovial cell function, we extended our study to cultures that had been sub-cloned to contain high amounts of trisomy 7-carrying cells. These cell cultures had approximately four times more versican than their disomic counterparts in the cell culture medium, indicating that versican may be a mediator in the processes of joint destructive disorders. To find an explanation for this increase in versican, we investigated the expression/secretion of PDGF-AA and IL-6, cytokines with their genes located to chromosome 7. Indeed, both these cytokines were increased in the cultures with high frequencies of trisomy 7. We then added the two cytokines to cell cultures of disomic synovial cells, but only cells treated with IL-6 displayed an increased amount of versican. Thus, we suggest that the increased amount of versican in cultures of trisomy 7-carrying cells relates to an autocrine loop involving an increased IL-6 production. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Trisomy 7, Versican, IL-6, Decorin, Synovial cells
in
Matrix Biology
volume
21
issue
4
pages
325 - 335
publisher
Elsevier
external identifiers
  • wos:000177237000003
  • pmid:12128070
  • scopus:0036069645
ISSN
1569-1802
DOI
10.1016/S0945-053X(02)00012-4
language
English
LU publication?
yes
id
61833325-e006-4dd4-a554-23349b617d24 (old id 109464)
alternative location
http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12128070&dopt=Abstract
date added to LUP
2007-07-11 16:07:01
date last changed
2017-06-29 14:07:49
@article{61833325-e006-4dd4-a554-23349b617d24,
  abstract     = {To gain further insight into the synthesis and structure of the synovial matrix of joints, we have established cell cultures from synovial specimens and elaborated their production of hyaluronan and proteoglycans. The cultures secreted mainly the small proteoglycan decorin, but also considerable amounts of the related biglycan and the large proteoglycan versican. Only minor amounts of heparan sulfate proteoglycans were found. All cultures also had a high production of hyaluronan, which highlights the important role for normal joint function of these cells. In joint diseases, a common feature is the presence of an extra chromosome 7 (trisomy 7) in the synovial cells. To study the possible consequences of trisomy 7 on the synovial cell function, we extended our study to cultures that had been sub-cloned to contain high amounts of trisomy 7-carrying cells. These cell cultures had approximately four times more versican than their disomic counterparts in the cell culture medium, indicating that versican may be a mediator in the processes of joint destructive disorders. To find an explanation for this increase in versican, we investigated the expression/secretion of PDGF-AA and IL-6, cytokines with their genes located to chromosome 7. Indeed, both these cytokines were increased in the cultures with high frequencies of trisomy 7. We then added the two cytokines to cell cultures of disomic synovial cells, but only cells treated with IL-6 displayed an increased amount of versican. Thus, we suggest that the increased amount of versican in cultures of trisomy 7-carrying cells relates to an autocrine loop involving an increased IL-6 production.},
  author       = {Eklund, Erik and Broberg, Karin and Westergren-Thorsson, Gunilla and Bjärdahlen, Anette and Hedlund, Maria and Malmström, Anders},
  issn         = {1569-1802},
  keyword      = {Trisomy 7,Versican,IL-6,Decorin,Synovial cells},
  language     = {eng},
  number       = {4},
  pages        = {325--335},
  publisher    = {Elsevier},
  series       = {Matrix Biology},
  title        = {Proteoglycan production in disomic and trisomy 7-carrying human synovial cells.},
  url          = {http://dx.doi.org/10.1016/S0945-053X(02)00012-4},
  volume       = {21},
  year         = {2002},
}