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Regulation of Ca2+ channel and phosphatase activities by polyamines in intestinal and vascular smooth muscle - implications for cellular growth and contractility.

Nilsson, Bengt-Olof LU ; Gomez, Maria LU ; Swärd, Karl LU and Hellstrand, Per LU (2002) In Acta Physiologica Scandinavica1888-12-31+01:002006-01-01+01:00 176(1). p.33-41
Abstract
Polyamines added extracellularly to intestinal and vascular smooth muscle cells cause relaxation through inhibition of Ca2+ channel activity. Intracellularly applied polyamines also affect Ca2+ channel properties. Polyamines do not readily pass over the plasma membrane because of their positive charges but in permeabilized smooth muscle preparations they have free access to the cytoplasm. In this system they increase sensitivity of the contractile machinery to Ca2+ through inhibition of myosin phosphatase activity. The magnitude of Ca2+ channel and phosphatase inhibition depends on the number of positive charges on the polyamine molecule. Polyamines have an obligatory, but yet undefined, role in regulation of cell growth and proliferation.... (More)
Polyamines added extracellularly to intestinal and vascular smooth muscle cells cause relaxation through inhibition of Ca2+ channel activity. Intracellularly applied polyamines also affect Ca2+ channel properties. Polyamines do not readily pass over the plasma membrane because of their positive charges but in permeabilized smooth muscle preparations they have free access to the cytoplasm. In this system they increase sensitivity of the contractile machinery to Ca2+ through inhibition of myosin phosphatase activity. The magnitude of Ca2+ channel and phosphatase inhibition depends on the number of positive charges on the polyamine molecule. Polyamines have an obligatory, but yet undefined, role in regulation of cell growth and proliferation. Several groups of protein kinases, such as tyrosine and mitogen activated protein (MAP)-kinases transmit the growth signal from the plasma membrane to the cell nucleus where mitosis and protein synthesis are initiated. The data reviewed here show that polyamines may affect such signal transmission via inhibition of phosphatase activity. (Less)
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organization
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Contribution to journal
publication status
published
subject
in
Acta Physiologica Scandinavica1888-12-31+01:002006-01-01+01:00
volume
176
issue
1
pages
33 - 41
publisher
Wiley-Blackwell
external identifiers
  • wos:000177595600005
  • pmid:12193217
  • scopus:0035982015
ISSN
0001-6772
DOI
10.1046/j.1365-201X.2002.01013.x
language
English
LU publication?
yes
id
f912113b-86b3-4a3b-b04a-3587d58a96d4 (old id 110066)
date added to LUP
2007-07-18 12:38:17
date last changed
2017-11-27 13:21:31
@article{f912113b-86b3-4a3b-b04a-3587d58a96d4,
  abstract     = {Polyamines added extracellularly to intestinal and vascular smooth muscle cells cause relaxation through inhibition of Ca2+ channel activity. Intracellularly applied polyamines also affect Ca2+ channel properties. Polyamines do not readily pass over the plasma membrane because of their positive charges but in permeabilized smooth muscle preparations they have free access to the cytoplasm. In this system they increase sensitivity of the contractile machinery to Ca2+ through inhibition of myosin phosphatase activity. The magnitude of Ca2+ channel and phosphatase inhibition depends on the number of positive charges on the polyamine molecule. Polyamines have an obligatory, but yet undefined, role in regulation of cell growth and proliferation. Several groups of protein kinases, such as tyrosine and mitogen activated protein (MAP)-kinases transmit the growth signal from the plasma membrane to the cell nucleus where mitosis and protein synthesis are initiated. The data reviewed here show that polyamines may affect such signal transmission via inhibition of phosphatase activity.},
  author       = {Nilsson, Bengt-Olof and Gomez, Maria and Swärd, Karl and Hellstrand, Per},
  issn         = {0001-6772},
  language     = {eng},
  number       = {1},
  pages        = {33--41},
  publisher    = {Wiley-Blackwell},
  series       = {Acta Physiologica Scandinavica1888-12-31+01:002006-01-01+01:00},
  title        = {Regulation of Ca2+ channel and phosphatase activities by polyamines in intestinal and vascular smooth muscle - implications for cellular growth and contractility.},
  url          = {http://dx.doi.org/10.1046/j.1365-201X.2002.01013.x},
  volume       = {176},
  year         = {2002},
}