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Contractile response in isolated human groin lymphatics

Sjöberg, Trygve LU ; Alm, P; Andersson, K E; Norgren, Lars LU and Steen, Stig LU (1987) In Lymphology 20(3). p.152-160
Abstract
Lymphatics from the human superficial groin removed at operation in 21 patients (one with lymphedema) were examined in vitro. Histochemically no nerves were identified with either specific catecholamine fluorescence or immunoreactivity to tyrosine hydroxylase or dopamine beta-hydroxylase. Ring preparations of the lymphatics were mounted in tissue baths and isometric induced contractions were recorded after administration of K+ (124 mM), acetylcholine, selected amines and prostanoids. Noradrenaline (NA), adrenaline, dopamine, and acetylcholine had no or only weak contractile effects. In some segments, serotonin induced contractions. Prostaglandin E2 showed no contractile effect and prostaglandin F2 alpha induced contraction in most of the... (More)
Lymphatics from the human superficial groin removed at operation in 21 patients (one with lymphedema) were examined in vitro. Histochemically no nerves were identified with either specific catecholamine fluorescence or immunoreactivity to tyrosine hydroxylase or dopamine beta-hydroxylase. Ring preparations of the lymphatics were mounted in tissue baths and isometric induced contractions were recorded after administration of K+ (124 mM), acetylcholine, selected amines and prostanoids. Noradrenaline (NA), adrenaline, dopamine, and acetylcholine had no or only weak contractile effects. In some segments, serotonin induced contractions. Prostaglandin E2 showed no contractile effect and prostaglandin F2 alpha induced contraction in most of the tested lymphatics. The prostaglandin-endoperoxide analogue U44069 uniformly elicited marked concentration-dependent contraction. In the lymphatic segment from the patient with lymphedema, a slightly greater contractile response to NA and serotonin was observed. The results overall suggest an absence of sympathetic innervation and contraction-mediating alpha adrenergic receptors in human superficial groin lymphatics, and support that certain prostanoids may be important regulators of human lymphatic contractility. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Lymphology
volume
20
issue
3
pages
152 - 160
publisher
International Society of Lymphology
external identifiers
  • pmid:3682939
  • scopus:0023578720
ISSN
0024-7766
language
English
LU publication?
yes
id
efc69878-785e-4259-8c74-ea644d31121e (old id 1103989)
date added to LUP
2008-08-08 17:23:12
date last changed
2017-01-01 07:08:22
@article{efc69878-785e-4259-8c74-ea644d31121e,
  abstract     = {Lymphatics from the human superficial groin removed at operation in 21 patients (one with lymphedema) were examined in vitro. Histochemically no nerves were identified with either specific catecholamine fluorescence or immunoreactivity to tyrosine hydroxylase or dopamine beta-hydroxylase. Ring preparations of the lymphatics were mounted in tissue baths and isometric induced contractions were recorded after administration of K+ (124 mM), acetylcholine, selected amines and prostanoids. Noradrenaline (NA), adrenaline, dopamine, and acetylcholine had no or only weak contractile effects. In some segments, serotonin induced contractions. Prostaglandin E2 showed no contractile effect and prostaglandin F2 alpha induced contraction in most of the tested lymphatics. The prostaglandin-endoperoxide analogue U44069 uniformly elicited marked concentration-dependent contraction. In the lymphatic segment from the patient with lymphedema, a slightly greater contractile response to NA and serotonin was observed. The results overall suggest an absence of sympathetic innervation and contraction-mediating alpha adrenergic receptors in human superficial groin lymphatics, and support that certain prostanoids may be important regulators of human lymphatic contractility.},
  author       = {Sjöberg, Trygve and Alm, P and Andersson, K E and Norgren, Lars and Steen, Stig},
  issn         = {0024-7766},
  language     = {eng},
  number       = {3},
  pages        = {152--160},
  publisher    = {International Society of Lymphology},
  series       = {Lymphology},
  title        = {Contractile response in isolated human groin lymphatics},
  volume       = {20},
  year         = {1987},
}