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A fibroblast heparan sulphate proteoglycan with a 70 kDa core protein is linked to membrane phosphatidylinositol

Schmidtchen, Artur LU ; Sundler, Roger LU and Fransson, Lars-Åke LU (1990) In Glycoconjugate Journal 7(6). p.563-572
Abstract
Here we present evidence that a fibroblast heparan sulphate proteoglycan of approx. 300 kDa and with a core protein of apparent molecular mass 70 kDa is covalently linked to the plasma membrane via a linkage structure involving phosphatidylinositol. Phosphatidylinositol-specific phospholipase C releases such a heparan sulphate proteoglycan only from cells labelled with [35S]sulphate in the absence of serum. Cell cultures labelled with [3H]myo-inositol in the absence or presence of serum produce a radiolabelled heparan sulphate proteoglycan which was purified by gel-permeation chromatography and ion-exchange chromatography on MonoQ. Digestion with heparan sulphate lyase and analysis by gel-permeation chromatography and sodium... (More)
Here we present evidence that a fibroblast heparan sulphate proteoglycan of approx. 300 kDa and with a core protein of apparent molecular mass 70 kDa is covalently linked to the plasma membrane via a linkage structure involving phosphatidylinositol. Phosphatidylinositol-specific phospholipase C releases such a heparan sulphate proteoglycan only from cells labelled with [35S]sulphate in the absence of serum. Cell cultures labelled with [3H]myo-inositol in the absence or presence of serum produce a radiolabelled heparan sulphate proteoglycan which was purified by gel-permeation chromatography and ion-exchange chromatography on MonoQ. Digestion with heparan sulphate lyase and analysis by gel-permeation chromatography and sodium dodecylsulphate-polyacrylamide gel-electrophoresis revealed that the 3H-label is associated with a core protein of apparent mass 70 kDa. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
heparan sulphate, phosphatidylinositol, fibroblasts, proteoglycan
in
Glycoconjugate Journal
volume
7
issue
6
pages
563 - 572
publisher
Springer
external identifiers
  • pmid:2136353
  • scopus:0025696364
ISSN
1573-4986
DOI
10.1007/BF01189077
language
English
LU publication?
yes
id
0b089702-86ad-49d5-9788-673c26d50649 (old id 1105413)
date added to LUP
2008-08-05 14:43:57
date last changed
2017-01-01 07:24:51
@article{0b089702-86ad-49d5-9788-673c26d50649,
  abstract     = {Here we present evidence that a fibroblast heparan sulphate proteoglycan of approx. 300 kDa and with a core protein of apparent molecular mass 70 kDa is covalently linked to the plasma membrane via a linkage structure involving phosphatidylinositol. Phosphatidylinositol-specific phospholipase C releases such a heparan sulphate proteoglycan only from cells labelled with [35S]sulphate in the absence of serum. Cell cultures labelled with [3H]myo-inositol in the absence or presence of serum produce a radiolabelled heparan sulphate proteoglycan which was purified by gel-permeation chromatography and ion-exchange chromatography on MonoQ. Digestion with heparan sulphate lyase and analysis by gel-permeation chromatography and sodium dodecylsulphate-polyacrylamide gel-electrophoresis revealed that the 3H-label is associated with a core protein of apparent mass 70 kDa.},
  author       = {Schmidtchen, Artur and Sundler, Roger and Fransson, Lars-Åke},
  issn         = {1573-4986},
  keyword      = {heparan sulphate,phosphatidylinositol,fibroblasts,proteoglycan},
  language     = {eng},
  number       = {6},
  pages        = {563--572},
  publisher    = {Springer},
  series       = {Glycoconjugate Journal},
  title        = {A fibroblast heparan sulphate proteoglycan with a 70 kDa core protein is linked to membrane phosphatidylinositol},
  url          = {http://dx.doi.org/10.1007/BF01189077},
  volume       = {7},
  year         = {1990},
}