Advanced

Early B-cell factor (o/e-1) is a promoter of adipogenesis and involved in control of genes important for terminal adipocyte differentiation.

Åkerblad, Peter; Lind, Ulrika; Liberg, David LU ; Bamberg, Krister and Sigvardsson, Mikael LU (2002) In Molecular and Cellular Biology 22(22). p.8015-8025
Abstract
Olf-1/early B-cell factor (O/E-1) is a transcription factor important for B-lymphocyte and neuronal gene regulation. Here we report that all three known O/E genes (O/E-1, -2, and -3) are expressed in mouse adipose tissue and are upregulated during adipocyte differentiation. Forced expression of O/E-1 in either the preadipocyte cell line 3T3-L1 or mouse embryonic fibroblasts augmented adipogenesis, and constitutive expression of O/E-1 in uncommitted NIH 3T3 fibroblasts led to initiation of adipocyte differentiation. Furthermore, a dominant negative form of O/E-1 partially suppressed 3T3-L1 adipogenesis, indicating that expression from endogenous O/E target genes is required for 3T3-L1 terminal differentiation. Thus, our data point to the... (More)
Olf-1/early B-cell factor (O/E-1) is a transcription factor important for B-lymphocyte and neuronal gene regulation. Here we report that all three known O/E genes (O/E-1, -2, and -3) are expressed in mouse adipose tissue and are upregulated during adipocyte differentiation. Forced expression of O/E-1 in either the preadipocyte cell line 3T3-L1 or mouse embryonic fibroblasts augmented adipogenesis, and constitutive expression of O/E-1 in uncommitted NIH 3T3 fibroblasts led to initiation of adipocyte differentiation. Furthermore, a dominant negative form of O/E-1 partially suppressed 3T3-L1 adipogenesis, indicating that expression from endogenous O/E target genes is required for 3T3-L1 terminal differentiation. Thus, our data point to the importance of O/E target genes for adipocyte differentiation and suggest a novel role for O/E-1 as an initiator and stimulator of adipogenesis. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Molecular and Cellular Biology
volume
22
issue
22
pages
8015 - 8025
publisher
American Society for Microbiology
external identifiers
  • wos:000178953700026
  • pmid:12391167
  • scopus:0036839672
ISSN
0270-7306
DOI
10.1128/MCB.22.22.8015-8025.2002
language
English
LU publication?
yes
id
d668653e-cd4c-4abc-8fc0-459195c327a9 (old id 110544)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12391167&dopt=Abstract
http://mcb.asm.org/cgi/content/full/22/22/8015?view=full&pmid=12391167
date added to LUP
2007-07-23 15:59:35
date last changed
2017-10-01 03:36:14
@article{d668653e-cd4c-4abc-8fc0-459195c327a9,
  abstract     = {Olf-1/early B-cell factor (O/E-1) is a transcription factor important for B-lymphocyte and neuronal gene regulation. Here we report that all three known O/E genes (O/E-1, -2, and -3) are expressed in mouse adipose tissue and are upregulated during adipocyte differentiation. Forced expression of O/E-1 in either the preadipocyte cell line 3T3-L1 or mouse embryonic fibroblasts augmented adipogenesis, and constitutive expression of O/E-1 in uncommitted NIH 3T3 fibroblasts led to initiation of adipocyte differentiation. Furthermore, a dominant negative form of O/E-1 partially suppressed 3T3-L1 adipogenesis, indicating that expression from endogenous O/E target genes is required for 3T3-L1 terminal differentiation. Thus, our data point to the importance of O/E target genes for adipocyte differentiation and suggest a novel role for O/E-1 as an initiator and stimulator of adipogenesis.},
  author       = {Åkerblad, Peter and Lind, Ulrika and Liberg, David and Bamberg, Krister and Sigvardsson, Mikael},
  issn         = {0270-7306},
  language     = {eng},
  number       = {22},
  pages        = {8015--8025},
  publisher    = {American Society for Microbiology},
  series       = {Molecular and Cellular Biology},
  title        = {Early B-cell factor (o/e-1) is a promoter of adipogenesis and involved in control of genes important for terminal adipocyte differentiation.},
  url          = {http://dx.doi.org/10.1128/MCB.22.22.8015-8025.2002},
  volume       = {22},
  year         = {2002},
}