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Isolation and identification from Salvia officinalis of two diterpenes which inhibit t-butylbicyclophosphoro[35S]thionate binding to chloride channel of rat cerebrocortical membranes in vitro

Rutherford, Diane M; Nielsen, Mogens P; Hansen, Susanne K; Witt, Michael-Robin; Bergendorff, Ola LU and Sterner, Olov LU (1992) In Neuroscience Letters 135(2). p.224-226
Abstract
Ethanolic extracts from dried leaves of sage (Salvia officinalis) showed inhibition of [35S]tertiary-butylbicyclophosphorothionate ([35S]TBPS) binding to rat brain membranes in vitro. This ligand is considered to bind to the chloride channel of the GABA/benzodiazepine receptor complex in brain tissue. Substances having inhibitory activity were purified and their chemical structure identified as the diterpenes carnosic acid and carnosol (IC50 values of 33 +/- 3 microM and 57 +/- 4 microM, respectively). The two compounds did not affect binding of the ligands [3H]muscimol and [3H]diazepam to the GABA/benzodiazepine complex in vitro. Saturation experiments of [35S]TBPS binding indicated that carnosic acid decreases the binding affinity.
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author
organization
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type
Contribution to journal
publication status
published
subject
keywords
γ-Aminobutyric acid, Benzodiazepine receptor, [35S]TBPS binding, Salvia officinalis, Diterpenoid, Carnosol, Carnosic acid
in
Neuroscience Letters
volume
135
issue
2
pages
224 - 226
publisher
Elsevier
external identifiers
  • pmid:1378214
  • scopus:0026613007
ISSN
0304-3940
DOI
10.1016/0304-3940(92)90441-9
language
English
LU publication?
yes
id
8c26a01a-4004-4fb3-bbe1-89bf91155ca7 (old id 1106616)
date added to LUP
2008-08-12 08:55:30
date last changed
2017-03-12 03:37:17
@article{8c26a01a-4004-4fb3-bbe1-89bf91155ca7,
  abstract     = {Ethanolic extracts from dried leaves of sage (Salvia officinalis) showed inhibition of [35S]tertiary-butylbicyclophosphorothionate ([35S]TBPS) binding to rat brain membranes in vitro. This ligand is considered to bind to the chloride channel of the GABA/benzodiazepine receptor complex in brain tissue. Substances having inhibitory activity were purified and their chemical structure identified as the diterpenes carnosic acid and carnosol (IC50 values of 33 +/- 3 microM and 57 +/- 4 microM, respectively). The two compounds did not affect binding of the ligands [3H]muscimol and [3H]diazepam to the GABA/benzodiazepine complex in vitro. Saturation experiments of [35S]TBPS binding indicated that carnosic acid decreases the binding affinity.},
  author       = {Rutherford, Diane M and Nielsen, Mogens P and Hansen, Susanne K and Witt, Michael-Robin and Bergendorff, Ola and Sterner, Olov},
  issn         = {0304-3940},
  keyword      = {γ-Aminobutyric acid,Benzodiazepine receptor,[35S]TBPS binding,Salvia officinalis,Diterpenoid,Carnosol,Carnosic acid},
  language     = {eng},
  number       = {2},
  pages        = {224--226},
  publisher    = {Elsevier},
  series       = {Neuroscience Letters},
  title        = {Isolation and identification from Salvia officinalis of two diterpenes which inhibit t-butylbicyclophosphoro[35S]thionate binding to chloride channel of rat cerebrocortical membranes in vitro},
  url          = {http://dx.doi.org/10.1016/0304-3940(92)90441-9},
  volume       = {135},
  year         = {1992},
}