Isolation and identification from Salvia officinalis of two diterpenes which inhibit t-butylbicyclophosphoro[35S]thionate binding to chloride channel of rat cerebrocortical membranes in vitro
(1992) In Neuroscience Letters 135(2). p.224-226- Abstract
- Ethanolic extracts from dried leaves of sage (Salvia officinalis) showed inhibition of [35S]tertiary-butylbicyclophosphorothionate ([35S]TBPS) binding to rat brain membranes in vitro. This ligand is considered to bind to the chloride channel of the GABA/benzodiazepine receptor complex in brain tissue. Substances having inhibitory activity were purified and their chemical structure identified as the diterpenes carnosic acid and carnosol (IC50 values of 33 +/- 3 microM and 57 +/- 4 microM, respectively). The two compounds did not affect binding of the ligands [3H]muscimol and [3H]diazepam to the GABA/benzodiazepine complex in vitro. Saturation experiments of [35S]TBPS binding indicated that carnosic acid decreases the binding affinity.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1106616
- author
- Rutherford, Diane M ; Nielsen, Mogens P ; Hansen, Susanne K ; Witt, Michael-Robin ; Bergendorff, Ola LU and Sterner, Olov LU
- organization
- publishing date
- 1992
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- γ-Aminobutyric acid, Benzodiazepine receptor, [35S]TBPS binding, Salvia officinalis, Diterpenoid, Carnosol, Carnosic acid
- in
- Neuroscience Letters
- volume
- 135
- issue
- 2
- pages
- 224 - 226
- publisher
- Elsevier
- external identifiers
-
- pmid:1378214
- scopus:0026613007
- ISSN
- 0304-3940
- DOI
- 10.1016/0304-3940(92)90441-9
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Department of Dermatology and Venereology (Lund) (013006000), Organic chemistry (S/LTH) (011001240)
- id
- 8c26a01a-4004-4fb3-bbe1-89bf91155ca7 (old id 1106616)
- date added to LUP
- 2016-04-01 12:26:46
- date last changed
- 2021-05-23 04:36:06
@article{8c26a01a-4004-4fb3-bbe1-89bf91155ca7, abstract = {{Ethanolic extracts from dried leaves of sage (Salvia officinalis) showed inhibition of [35S]tertiary-butylbicyclophosphorothionate ([35S]TBPS) binding to rat brain membranes in vitro. This ligand is considered to bind to the chloride channel of the GABA/benzodiazepine receptor complex in brain tissue. Substances having inhibitory activity were purified and their chemical structure identified as the diterpenes carnosic acid and carnosol (IC50 values of 33 +/- 3 microM and 57 +/- 4 microM, respectively). The two compounds did not affect binding of the ligands [3H]muscimol and [3H]diazepam to the GABA/benzodiazepine complex in vitro. Saturation experiments of [35S]TBPS binding indicated that carnosic acid decreases the binding affinity.}}, author = {{Rutherford, Diane M and Nielsen, Mogens P and Hansen, Susanne K and Witt, Michael-Robin and Bergendorff, Ola and Sterner, Olov}}, issn = {{0304-3940}}, keywords = {{γ-Aminobutyric acid; Benzodiazepine receptor; [35S]TBPS binding; Salvia officinalis; Diterpenoid; Carnosol; Carnosic acid}}, language = {{eng}}, number = {{2}}, pages = {{224--226}}, publisher = {{Elsevier}}, series = {{Neuroscience Letters}}, title = {{Isolation and identification from Salvia officinalis of two diterpenes which inhibit t-butylbicyclophosphoro[35S]thionate binding to chloride channel of rat cerebrocortical membranes in vitro}}, url = {{http://dx.doi.org/10.1016/0304-3940(92)90441-9}}, doi = {{10.1016/0304-3940(92)90441-9}}, volume = {{135}}, year = {{1992}}, }